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CRIMIC CR00846

Gene cni

CG5855

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:16310229..16310242	35F1	FBti0199643	79315

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGGGTCTGGAATAAGGATGCCCTTA	CAATCCGTCTCCAAAAATCGCTGGCT

Images

Construct Design Sequences

sgRNA sequence	ATAATGTGATTTGAGATCCGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCGCTGTCCCTCATGCTAAA	TGAGATCCGAGGAGATCAAGA	TCACACGGTCAAATGAGTCAC	AGATTAGTGCATCCCTGGCG

 

Amplicon 5'

Amplicon 3'

CCGCTGTCCCTCATGCTAAACAGAAAAATGAAAATTGGTTACATTGATTTGACTTTTATTTAATGCTTAAAATCCAACTACACCAAAGATGCGATATGCAAAAAATAATCAAAATAAAAACATGTTACAATCTCTTACTGCAGAAAAGTATTACAAATTGTATGAAAAAAACAAAGCTTCAAGATGTCTTTATGGATTTACACTAGAATTTACAAGTGAAGTATTGTTCGCGGTTGATGGGGACTCTTCCCCAAAAAAGGAAGTCCTCTACCCGAGTTTAGACAACTAATACACGACGCTGATGCGTTGGCGCCAACACTGAGATCCCCAGGCAAGAGCTTGCCTATGTCGAGATGAGCGAATAAACCATGCTAAAATTAGCAATTAGAAACGTTAGACAAATGAGGACCCTGGCCACAATGAATCTCAATTAGACTTACCCGTATATGTAGTAGAAGAAGCTAATCAGATAGACGGCCAGTTTAATCCAACCCTCACGCATGTTCCGGTACAAAGTATCGGTTTTCAGGACCGTGGTGGGATCATAAAGTCCGGGTCCCGACATCACAGGGCGGTTTTTGTAACTGAAAGCGATAGGGGTATTACAAAGCATGAGTGCTTAAGGGTGCTTATGTTGGGCTTGGGTGAATCTTGATCTCCTCGGATCTCA

TCACACGGTCAAATGAGTCACTTACCGCCAAATATGATAGGCTATGAGGGGTATGTTGATGCATAGCGAAAACCATTCGCCGCAGAACAAGAAGAGCAAGTTCAGAAAGATGTGCAGAAGGTATTCGGGCAAAACCAGCTGAAATGCAAACACATACATTACAGGGCGCACTGCGGGTGTAAGCAGTAAGTTTACTGACCGGATTGAGGCTATTGCACTGATCAATTGGGTTCTTATAATCCGTTTTAAGTTCGTCGAATGCAATGACATGAAATATGGCGAAAAATATCAAGAATGCATCCCCGATCAGGGCCACTATGTACGTGAAGGCGGTGAAGTTGAAGGCCATCTTGTAAAATCGCTGGAGCACGTCTTCCAGGTGCTAATCAAAGTGACTTGCTAATTATTATCGTAAAATTCGGCTTTGCAATTGTAGCGCTGGGACTCACTTGTTTTTTTTTTACAAGGGCGGCGATGATGACAGCTGTGCGCCAGGGATGCACTAATCT

 

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