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CRIMIC CR00847

Gene dare

CG12390

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:11292480..11292507	47F1	FBti0199644	79316

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTTACGCTTACAGAAGCACCCGAA	AGAACTGGTGTCCAGAGCATTCGTGT

Images

Construct Design Sequences

sgRNA sequence	CGTGCACACGCAATCGTGTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTGGGCAGCTTGAGCATCT	ACGGCATTCCATCGCACTTA	GCATTTGATTTCGGAGTGGTT	CAGAGATCGGCGACTGATCC

 

Amplicon 5'

Amplicon 3'

ATTGGGCAGCTTGAGCATCTCCCTCAGCTCCTTAATGGTGAAGGCAGCTTGCAGAGGACCTCGTCGGCCAACCAAATGGACTCGTTCCACTTGGCTGCAAGAAAGCGCTTCCAAGGCGTACTCAGTTGTGTCCGTAGTCTGTAACAATGCACATGAGAATCGTGGAATTGTTTGGGTATTAAAAAGAAACTCACCTTCAAAGCATCCAAGGGACTGAGCAGCATCCTAGCCACATCCACAGCCACGTTTCCCTGACCCACAATCGTGACATCGCGACCGCTCAGATCCGGAGCCAAGTTTTCCGCACCCGGCAATCCATTATACCAGGCCACAAATTTCCGGGCTGATATCACATTATCCAACTGCTCGTTCTCCAGCTCTAGTTGCCGATCCTGGTCCGCTCCGTAGGTGAGGAGCACGGCATGGTATCGATCTCTTAGCTCACGCAGGCTGACATCCGTACCCAGCGATATGTTGCCAAAGTAACGCAGACGCGGATGCTCGGCGGTCTTGGTGAAGGTGTTGATTACGTTCTTGACCTCCGGATGATCGGGCGCAACGCCAAAGCTGCAATGTGCGATTTGCATAATGGGACAAGTCGTTAGTCGATAAGTGCGATGGAATGCCGT

GCATTTGATTTCGGAGTGGTTGTCACAGTTTGTATTCCAAGATGCAGGTGCTAGGCGGCAATCCACTCAGCTTGTGTCTCTTGGTGATCATCACTCCGTCACTGATTGCAGCCAATCCCCTGTGGAGTCTTGACCAGGTGGGAGGTATGTTTACATTACCCCTGGATTTCTGGCAAGGATTACACACATGTCGATCTCTTCAGCATACTTCACTGGCATCTTCCGCTCCGTAGTGGGTCCAATATTTGGCTTTGGCCTGGCAGCCAACAGCACCGCCTTATAAAGATTGTTCTGCTTACCGCACCAGTCCGAAGGGAACCGGCAGCTTCTCGACCACATCCACCACGCAATTGTCCAGCTGCTTGAGGATCAATTGGGCGGCATAGAAGCCGGCGGGTCCGGCGCCTACGATGCATATCCGCTTGGTGGGCGTGGTGCTCTGGATCACCTGCAATCGGGCGGAACTCGTGTGGAGGCCACGTCTGAAGATGTTAAGGCAATTGATGCCCATCGTGGATCAGTCGCCGATCTCTG

 

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