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CRIMIC CR00848

Gene lig

CG8715

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:8073105..8073116	44A4	FBti0199645	79317

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAATGCCCGGAGACAATGCACTGAA	CTAACCATTTGGATGTTGGTGGGCAC

Images

Construct Design Sequences

sgRNA sequence	TTAGGTGCGGAGCGCGATTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GACCAAGAGCGATCCCTACG	TGCGGTTTGGGATTTTTGGG	GCACCTAACAGCGGCAATG	CCGACTCCACTACTTACGCC

 

Amplicon 5'

Amplicon 3'

GACCAAGAGCGATCCCTACGGACAGACGGGCGGCAGTGGTAACGCCTACCAAAACGCGTACCAGAGCAGTGGAGCGAGTAAGACAGCCAGTGGCTTTCCGACGACCGCGCCCGGTGGCTACAGCAGCTCCACATACGCGAATGTGCAAAGCTCAGTAGCCAACAGTTACCAGCAGCAGGGATACGGCTCATACCAGCCCAGTTCCTACCAGCAGCAGGCTGGCAGCGGGGCTCAAAGCGGTACAGGTGCGGTAAGCGGAGGCGGAGGAACGGCGACGCAAAACATTCCGGTTGGAGGCAGCAGCAGCCAAAATAGCACGAGGTATGACATGATCCAGTATTGATTGTTTAATATTTAATTCCAAAGGGTACGTACCCTTGCAGAGATTCGTAGTTACATATTTCTTAAATTTAAAAATTATTTCTAAGCAACAAGTCGATTTGGCAAGGAACAACTAACTATACTCAGCAAGGGTACAAACCTTATCGAATATATCTTTTACTTCAAAGTTTAAATATTTGTGTGTATGCAAGTTTTGCTTAACAATTAGCCCCACCCGAAAGAGAGCTAATCTTCATTTTGTTTACTGTTTTTCTTCTCTCCTAACACATTTAATGCTCGCTTATGTCCCAAAAATCCCAAACCGCA

GCACCTAACAGCGGCAATGCGAGCTCTGCCTACCTCACATCCGGATATTCGACACCACAAAGTGCTTACCAGTCAAGCCAGAGTGTTTATGGCAACACTGGACTGTCCAACAGCAGCGGGTAAGTACCAAATGTGACGATCCCAATCCCAATACGATCAGTTCCCTGGGGATGACACGTACCTTATTTTGCTTGTTTGGACTGGTTCTGGTTCCATTATGCTTAATTGCGGCTTCTCGCCCGTTCGTTCAGGTTCTCTGGCAGTGCGAGCAACGCGTCCTCGCAGTACGCCAATTTCAGTGCCAGCGCCAAGCTAAAGGATGCGACCACGGCCAGTAGCGCCGCTCATTACGACAGGTAAGAGCGAAACCCCCGCTCTGCATGCCCCGCTTTTTCAAATATTCCGACTAACCCAACCCCCTGGTCATATCCCACTTAGAGGCGGATGCCTGTAGCACTTAATGTTGATCTTATTGACCTTTCAGTGTCTCAACCAGCAGCGGAGTGAGCAGCAACAGCGGAAGCACAGGCAATGGCGGTGTGGTGAGCGGTCAAACAGGTGCTAACCAGGCGGCTGTATCGAACAGTAAGTAATCGATGATTTAATATCAAACAGTTGAGGCCCTAAAGAGTATGCATTGCAGTCATTAAGCTTTCTGGTATAGAAATCCCATATCAAAAATATTTAAAAACAAACTCACAATGTCAAATCTACTTCAATCTAGCCACGGCTTTGACAATACAACTCTCCAAAATAAATATAATTTTTGATTCAAACTTTTTGTTTAGCTGAAATGCTCTATTTTTGGCCGAATTCTTAGTATTCGCATTTAAACTAAAACCTTTCCGTCCTCTCCAGATAACAGCGTGAGCGGCAGCAGCTCGGTCAGCAATGTGACGGCAGGCGTTGCGAGTGGCAACGTAGCTGGCGTGGGCGGAGGCGTAAGCCAGAGCGGCGTAAGTAGTGGAGTCGG

 

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