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CRIMIC CR00849

Gene Dyrk2

CG4551

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:14192338..14192370	35A2	FBti0199646	79318

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACACATTTCGCACACACACACCGCAT	TCGGCGAATCGGGACGAAATACATGT

Images

Construct Design Sequences

sgRNA sequence	TTGACTGTACCACTGTAGCACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTTTTTCGTTGCCGTCGG	TCGCGGTGGAAGATAGAAGC	GCTGACTGCTCGGGAAAT	CCAAAGTGCCAAAGACAGCC

 

Amplicon 5'

Amplicon 3'

AACTTTTTCGTTGCCGTCGGGTAACTTTTAGCATAAATTTGAAATTGGTTTCCCTGCTGGCTGAAATCGAAAAGAGTGTGCCATTGATTGACAAGTGAGTGTGGTGTGGAAAAGCTGTCCTGTCTTCATTTGCATAGCGAATTTTCACGTGTGAAGAATGTTGAAGGCGGAATTAATTTTGCAGCAATCGATTCCACACGACACCATTCGCATAGCACTAATGAGATTCTGGCCATAAAGTTTGACTACAGCTGTGCTTATATATTGCCTTATTTTCTCGTTAATTCCCAAAATGACTATAAATATGCAGAATTAAGAGGCTTAACCAAAAGGATGCCTATCCATTAATGATGCTGTGTCCCTGGCTGGGACATTTGTGACCTTGCCTTTCATTCGCCCAAGGACCGGCTAAATTTTCTCACAAAAATATCCGTTTTTTTTTTTGCCCATCGCATTTTGCGGCATTTTAATTAGTCGACGGCTTACTTCGCAGTTGAACCGTAAATAATCTCAGAAATGCAGAGAAAAATTCCTGCCACAGCCCGGGGAAGTGGTGTGGAACTAGGGGCGAATTTACCTTTTAGTAGATGATAAATGACTTGGCCTTGGCCATGTCAACTGGCTTCTATCTTCCACCGCGA

GCTGACTGCTCGGGAAATGTCAATTAGCAGTAATTACTGTGCCATGGTACGGCATAATAATAACCTACATCAACATCCGTGGAACAGCTGACACCGACACTCCTTTGCCCCGCGTCCAAATGAAAATATCGCCCCCATCGAGTGTTTATTCTCCTGGTAACTCAATCCGCCGACTCGTCCTTGCCGCCCGTTCCTAATTGCGATTTACACGCGCTGAGTGCAAAAATTAATTAAAAAGAATAAACTTGCAGGACCTGCTCATTTGCATGGTCACGCCCACAGCTCACGCACGCCGCATGTTGCATTTTAATTAAAAATCATTTCGTCCTGTCGGCCCATCCGTTTGGCCCACATGACCAAAAGGCAGCGGAATGCCGACGACATCCTGTCACCTTTCTATGTCACTCTCACGTTGTCAGCGGCCATGTCCTGGAACAGTCAATTACATGGCTGATGACAGGCGGTCACATGCTGCATCTGTGCGACATTGATTGGCTTTGAAATGGATAAGTCGACGAGGTTTGCAAAAAGCAATTGCTTTCCGATCGATTGGAAAATACAAAATCACAAAATATGTGGTCAATATGTTGACTAATTACCTCAACTTAATCGACTTTAAGTGAACTCCCTCCGGACTTTTTAATTGCATTTTCGTTTCGTTTTTGGTTTAATTCGAATATGAAATTAGTAATCTCATTTATTTTATCCCCCTGTCGATCAATATTTATTGCCAATTGCTTTATTTTATCAATAATGAGCAAATCCCCTTAAACTGTATGCACTCGTTAATTTGAGCAATAAATTCTGGGGTGATTATCGCCAACTTGATCTCCTTATGAGCCATTAAAATTGAAATCAAATCATGACACATTGCATAGCGAAAACCCGCCGAGTTGAGAGAGAGGAACGCAGTGACATTGGAGGCGGAGCAGTCAGGCGCGGCAGGACACTTTCCTCCCGGGCAACATTAGCTGAAATTTATGTGCTTGACACACACCCACAGACACAAACTCACACACACACACACACAGAGCGAATTTCGCTATATCCACGAGGCCACGAGGCTCGAGCATTTTCTTTTGGCCAAAAAGAAGCAGCGTCATTCTCGGTGGGACAATATGGTGCATAGTTATGGGGGCTCGGAATGTGTTGGCTGTCTTTGGCACTTTGG

 

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