CR00850

CRIMIC CR00850

Gene spen

CG18497

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:183438..183458	21B4	FBti0200704	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	CTGGCAAGCATCATGAACATTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGAAATCGGGGTGGTCAGTG	TGCCAGAATAGTAACCCACCT	ATGCGATGAACATACATTCGTAG	AAGATCCGGCTCCTACCACT

Amplicon 5'	Amplicon 3'
TGAAATCGGGGTGGTCAGTGAGCGAAATGCGCCGAGCGAACGAACTGTTTATTAAATGAAGAGCAAATCGCAAGGACAAATAACGTAAGGCAAGCCTTTAACCGTGTAAGAGTGACGGAATAATAAAATATTTAACACTAAAAACCGGTTGGCTATTAAATATAGCAGCAAAGAAAAACGTGGCTCTCCCGGCTTTTTTGCTTCTGTGTTTGTGCTTCTTCGTAGCAGCTTTCGTGTGTGCAAACGCATCAGTGTGTGAGATATTTGTTTGTATGTGTAGCCGCGTAAACAGACGCAGACAAATAAAGGAAGAAGTTGGCGGATTGAGCCGCACGTCAGTTTATTTGGCGCTCGTTTATTTATTTGGCCAAGTGAAATTTTTCGTAGTGCCGATTTATCAATAGTACGAAGAAATCGGGAACGGATAAGCGAACAAAAAACGATAGATTTGGTACATTACTGTTTCCCGATGTTAGCACTAAATGTTGGGGCTCCCGTTTCTGGGGATGATAAATTAAACCGGGATTACCTATGTAGCAAAGCTTACAACGACGATTTTCCCAGCGAGAACCGGTAAATAATATGCGAAGGTGGGTTACTATTCTGGCA	ATGCGATGAACATACATTCGTAGTTAAAAAAAAAAAAACAAAACAATGTGGTGGCAACCCTAGTTCAATATATACATATGTATGTATGTACTATATGTATATGCGTATATGTTTTTACCTAGATAATAAAAAAAAAAGTGTTACAAACATATAAAGAAAAAGCAGTAAAAGCTAAATTAATTATGCGTATTATTCTATGTCATAAAATGAAAACGTATTTTTGCCCCTTATTTGTGCGCTATAACAAATACATACATTTTAATGTGTTTCTGAAATTTGATGAATTGTTCTACACTATTTTAGGTCTACAGAGGAACCACCGACAAACAGCTTCGAACGGAATTATTACGACCGCACCACCAGCCGTTTAGTCACGCAGTATCAAGCGAACAACTCAACTTCCTTGGCCAATTCAAACTCCAGTCCCTCGTCCGTGAGCGCCTCCGCGTCAGTCTTCGCTACGGCGGCAGGAGGGTCTTCCGAGAGATCACGCAACCGAGATCGCCCGTACAGGAATGGTTCTGCCAGCGTTCAAGGCGGCGGCATCAACAGCAGCAACACGACGACGACGACAGCAGCCTGCACAGCTGGAGGATCTGGATCGGGAGCAATTGGAACTGGGACTGGGGGTTTGGTAGGCTCAGGTCCTGGGGGAGTACCGCAGGCGCTAGGCGACCGCAGCAGCACCCAGAATATCCACCAGAACCATCAGAGCGCGCGGGTGGCTCCACCACAGTCATGGTACGAAGCCGCCACTGCTGCAACGACGGCCCAATTGAAAAGTTCTGGTGGCAGTGGCAATGCCGGCGCCTCAGCAGCCGTGGGCTTCACAATGAGCAGCTCACCGATCAACCATCATCCGCACCAGCATCCGCATCTTCAGAATCCTCAACACCCGCACTACACCAGCAGTCCAGTGGTAGGAGCCGGATCTT

Amplicon 5'

Amplicon 3'

TGAAATCGGGGTGGTCAGTGAGCGAAATGCGCCGAGCGAACGAACTGTTTATTAAATGAAGAGCAAATCGCAAGGACAAATAACGTAAGGCAAGCCTTTAACCGTGTAAGAGTGACGGAATAATAAAATATTTAACACTAAAAACCGGTTGGCTATTAAATATAGCAGCAAAGAAAAACGTGGCTCTCCCGGCTTTTTTGCTTCTGTGTTTGTGCTTCTTCGTAGCAGCTTTCGTGTGTGCAAACGCATCAGTGTGTGAGATATTTGTTTGTATGTGTAGCCGCGTAAACAGACGCAGACAAATAAAGGAAGAAGTTGGCGGATTGAGCCGCACGTCAGTTTATTTGGCGCTCGTTTATTTATTTGGCCAAGTGAAATTTTTCGTAGTGCCGATTTATCAATAGTACGAAGAAATCGGGAACGGATAAGCGAACAAAAAACGATAGATTTGGTACATTACTGTTTCCCGATGTTAGCACTAAATGTTGGGGCTCCCGTTTCTGGGGATGATAAATTAAACCGGGATTACCTATGTAGCAAAGCTTACAACGACGATTTTCCCAGCGAGAACCGGTAAATAATATGCGAAGGTGGGTTACTATTCTGGCA

ATGCGATGAACATACATTCGTAGTTAAAAAAAAAAAAACAAAACAATGTGGTGGCAACCCTAGTTCAATATATACATATGTATGTATGTACTATATGTATATGCGTATATGTTTTTACCTAGATAATAAAAAAAAAAGTGTTACAAACATATAAAGAAAAAGCAGTAAAAGCTAAATTAATTATGCGTATTATTCTATGTCATAAAATGAAAACGTATTTTTGCCCCTTATTTGTGCGCTATAACAAATACATACATTTTAATGTGTTTCTGAAATTTGATGAATTGTTCTACACTATTTTAGGTCTACAGAGGAACCACCGACAAACAGCTTCGAACGGAATTATTACGACCGCACCACCAGCCGTTTAGTCACGCAGTATCAAGCGAACAACTCAACTTCCTTGGCCAATTCAAACTCCAGTCCCTCGTCCGTGAGCGCCTCCGCGTCAGTCTTCGCTACGGCGGCAGGAGGGTCTTCCGAGAGATCACGCAACCGAGATCGCCCGTACAGGAATGGTTCTGCCAGCGTTCAAGGCGGCGGCATCAACAGCAGCAACACGACGACGACGACAGCAGCCTGCACAGCTGGAGGATCTGGATCGGGAGCAATTGGAACTGGGACTGGGGGTTTGGTAGGCTCAGGTCCTGGGGGAGTACCGCAGGCGCTAGGCGACCGCAGCAGCACCCAGAATATCCACCAGAACCATCAGAGCGCGCGGGTGGCTCCACCACAGTCATGGTACGAAGCCGCCACTGCTGCAACGACGGCCCAATTGAAAAGTTCTGGTGGCAGTGGCAATGCCGGCGCCTCAGCAGCCGTGGGCTTCACAATGAGCAGCTCACCGATCAACCATCATCCGCACCAGCATCCGCATCTTCAGAATCCTCAACACCCGCACTACACCAGCAGTCCAGTGGTAGGAGCCGGATCTT

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