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CRIMIC CR00851

Gene SamDC

CG5029

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:10339651..10339652	31D9	FBti0200705	81148

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TACCTGTGAAAGTGCGGATCTATCCG	TCCTCATACCACAGTAGCGCCATTAG

Images

Construct Design Sequences

sgRNA sequence	AAAGGACATGTCGGCTGTTTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCACGCAATGCAAACTTTTCA	CTGTTTGGGTTCCGCACTTG	CCGACATGTCCTTTGTGCAC	GGTTCATGGAGCCCAGACAA

 

Amplicon 5'

Amplicon 3'

TCACGCAATGCAAACTTTTCAAGGAAATATTTAAATTATATACAATTATAAGAAACTAAAAAACTTAATGCTGTTTTCGAATCTACATAAATTGTCTAAATAAAATAAGGACTTCATAATAGGCTAAGATACAAAAAATATTACTCATTTATTTTTTCAAATAAAATACTCTATACAATTTTTAACAATCACAATAAAACTGGTTAATGTAAAATGAAAATCCAAATTCTACTGTTTTTTTTTAACTATTTGCCTGGGGTATGGGATAAGCAACCGAAAAATCTGTTAAACTAATGATGAGAATTTGAAAAAATATATATTAAAGATATTTAAGTATCTTAATTCGAATAGAAAGGGACTTACATTTTTACCGCCGACTGTTCAATGAGAAATGCTGTAAGTGGAAACATGCTGTTAGACTCCCGAATTATCGATGTAGTACAACAAGCACTCGCATCGATAGTCCCATCGTTTACTTTACATCACCAGGCGTTGTTTTGGTATCAGATCAGATCCATTTGTTATTGCCAGCCAGCAGCATGTTGGAGAACGGTTCGCACTTCTTCGAGGGCGTTGAGAAACTCCTTGAGATTTGGTTTGAGGAGAGCTCCAACGGTGACGATGATCTGCGTAACATCAGCAGGTGAGTCCCTCAACAAGTGCGGAACCCAAACAG

CCGACATGTCCTTTGTGCACAAGTGCATGCACAGTTGCGGGCAAAAGTATACGTACATATGCGCCGTTCATTTTCGTCCTGAAAAGAAATGAAATGTATTTCTACACTGATAATTATCTTTTAAACATAACATAGTCCCATTGGAATAGGTTACACATTGGTTTTTAGGCTTATCAGATGCGGCACACGTGTACCTACCCAAATTAAATTGAAGTTTTTATTTATGATTCTCAAGATATACAAATTTGATTTGAAATTTCAAGAAATTAGTTATTAAAGGGAATTTCTTTTTAAACTGGCAGCCTGTAGAACTTTGTAAATTCACTTAGAATTAAGCTTTCAAAAATCGGTCTTAACCCTTGGCCTTGTTTGATAACGAGTTTTAATCAATCCCATTAATCCAATCCTCATTATCTCAAAATAAATTTTGGTCGAGGCATTGTGTACATGTTTATTCAAATAGCCTTGAACTTTTAATGAATGTAAATGATATTTTTCAGATCTGATTGGGAAAACGTGCTTAGCAATGTCAACTGTCAAATAATAAGCACATCCAAAAACGACATTATTGATGCTTTTGTGTTAAGGTATGCGAATATATAAAACTAAACTTAATAAAATATTATTAATTATTTTAAATTTGTATTCGGTGCGAGCAGTGAGAGCAGTATGTTTGTTTCAAAGCGACGATGGATCCTTAAAACTTGTGGAACGACTACTCCATTGAAATGCCTGGGTCAGTTGCTTAAGCTGGCTGAGGCCAATGGATACAATGTGGTCGCAGACTTGTTCTACTCGCGAAAAAATTTCACTAGACCGGAGGCGCAGGTGGGCAAAAAAATTAATTGCATTTCAAATGATTTTATATCATATATATTCAGATAACTCCCCATCAAGGCTTTACGGAGGAAGTTACGTATCTGGACTCAATTTTTCCCAACGGAAGATCCTATTGTCTGGGCTCCATGAACC

 

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