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CRIMIC CR00852

Gene CG5554

CG5554

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:23845001..23847024	60A3	FBti0199647	79319

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAGACACTCTTCAGACCAGTCAGA	CTGATGATCGCTAAGGCTGAAGATCC

Images

Construct Design Sequences

sgRNA sequence	ACGCCCCTTTATGTTACGTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCACTGGCTGCAGGTAGTTA	GGGGTTTCATGTGGGTAGGG	AACATAAAGGGGCGTGGTGC	CAAAGTCCACAAGGCACACG

 

Amplicon 5'

Amplicon 3'

GCACTGGCTGCAGGTAGTTATGGGCCATTTCTTGTGGCGAGGGAAACTTCCAAAATGTCGAATTTTGTTATGTTTTCGTAAACTTTTATTTTCTTTGTTGTTGTCTGGAGTTATCGATTGCAATAGCGATGGTACAAATGTAGTATTTGTATGCAGTTCAATCGACTTATAAATAGCTTGACATTGCAGAGGCTTCAATAAAATAAGTTATTATAATTAAAATGATTTTGAAGATACGATAAAAATTGAATTTGTATGTAAGAAAACTTGAAGCTTGATCTAATAAATAAAAAATCGTGTAACGAGCGAATACCGACCGAATCCCCCTACCGATGATCTAGCGGGGAATAAAGCGGCTTGCCATCTCCGATGGATATCGAACCAGCACTTCTGTCAATTTTCCAGCAGGCGTCGCAGTTTCTGTAACTTTTTGGAACTGACCAAGACGGAAGCTTTAGTTTAATTTAAGAAACTGTCGCAGCCGAACGTCACAGGTTTCAGGACACGAATCGGCGGAGCAAAGCACCAAAACATCACCACATGCATAGCACATCGTACTTTGTGGCCGCGACATGCGCGGCGCTGCTTCTGCTGGCGGCGACGGCGCAGTCGACGGCAGCAGCGCAGTCAGGACTGCAGCCAGGTGGCAAGTTAATCGAGTTGGACGAGGACAACTGGCACCTGATGCTCCAGGGCGAATGGATGATCGAGTTGTGAGTGCGAAAGGGTGGCTCTGAGTAGTCCCTACCCACATGAAACCCC

AACATAAAGGGGCGTGGTGCGCCGGCTTTCTCCAGCCGGCAAGCTGGTTCTTTCGTTCTCCGAAATACCAGACATTCCTTTGCGGCGACGATGACTCATCGGCAGCTGTGTTAAACATATGTTTCTCCCTCCATTCACAGCTTTGCTCCATGGTGCCCGGCCTGCAAGAACCTGGCTCCCACCTGGGAGCGGTTTGCCCGCGTGGCCAAGGATGTCCAGGTGCAGGTGGCCAAAATCGACGTGACAACCTCGCCCTCGCTGAGTGGACGCTTCTTTGTCACCGCCCTCCCCACCATCTACCAGTGAGCACCACATAGACCTTTTCAACCCATGAAATACACATCGTCGTAATCCACGTCAATCATTTCCAGCGTAAAAGATGGCGAGTTCCGGCAGTATCGTGGGGCCCGTGATGGCGACGCCCTCCTCTACTTCGTGAAGAAGCAGCAGTGGCAGAGCATCGAACCGCTGTCCGCCTGGAAGAAGCCCGACACCACCCACATGTCCGTGCTGTCCTACTTCTTCAAGCTTTCGCACACTCTGAAGGTACGTTCACCTAGCCCATTATCGCATCGGTTCACTGCATGAGACATAAGCGGAAGCTTATCAGCGGGTTGATCCCGTTGACCCACAAAAGATTGCCGCCCATCACTCATTACTCTGGTCTTTGCTCTCTGCTGTTGTCTTGTATGTTGGGTTCATGTACTAATAGCATACGCAAAAACTCTTTAAGGACTTCAACGGGCGCCTGCAGGAGGAATACGGACTGCCCACGTGGGGCTCCTACGCCCTCTTCGCCATCGCCACCATCTTCGTGGGCGCTGCCCTGGGGCTGCTGCTCGTGTGCCTTGTGGACTTTG

 

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