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CRIMIC CR00853

Gene CG31908

CG31908

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:7026028..7026049	27D4	FBti0199648	79320

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGTTCGATTACACAGTCTATCGGAC	GAATCTCGAGCTCAATGATTTGCGGG

Images

Construct Design Sequences

sgRNA sequence	TTTACCATCGCAACACTAATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGAATCTCAGAATGCCGCGT	GACCTACCAGGATCTTGCCG	GTTGCGATGGTAAATATTGGTCA	AAGACTAGCCCACGGAAAGC

 

Amplicon 5'

Amplicon 3'

TGAATCTCAGAATGCCGCGTGGGTTGCGCTTTTCGTTTGATTTTAATTTTTATATATATATATATGTAGAAACATGCATTTGGAATCGAAGTACCAAGTTGAAAATTAGAAGTTATTGAAATAGACAGGACTTGCTCCATTGCCGCAGTCTGTTACCGGCGCATCTGAAAGCCAATGGAGCCATTCGGACAGGGACCCTTGGGCAGGCGCAGGACTAGGTCGTTGAACGGATGCGGATGCTGGCCGAGCTGCTGCGCCTGATGATTCTGATGCTGCTGCTGATGCTGGTGCTGCTGCTGATGCTGCTGCTGGTGCTGCTGTGGCGACTGCAGCGACTGTTGGCGCAGGAGGGCGGCGGACACTGATCCGCCCACAACAGGTGACCAGCTCTGCTGCGGATTGGGCGACGACTGGGGCGGCGCCTGAGCTTGCATCCCAACACCGCCCAGTCCCGATCCTCCGCCTCCGTACGGCGGCAACCGTTGATTGAAATGCTGCGACGGCTGCTGCTGCTGTTGCTGCTGATGCTGATGCTGATGTTGCGGGTTATGCGGATAGTAGTAGTTAAGGCCAGGCTGATTGTGCAGTCCCGGGAAGTAGCTGTTGTAGTTATTATACATCGGCGTCACTTGGGACATTTTCGGTGCCGAACTCGAGCGGTGCATTCCGCCTCCTGGATGGCTACTATTGTTGTTGATGACGTTGTTGTTGCTGATCGCAGAGGGGGGAGTGACAGGCGGCGCCGCGGACGAAACAGCCACACTGCTGCTATTGACAGCTGGCGTGATGGGAGTGGCCCTAAAAAAACAACGAATATGTGCATATGTCTGATTAAATGTGGGTTTCTGTTTAGATATCTCTATAAATTAGACCAAATCATAAAGAAATATTCTAGTATCAAGAAAATATTTATAACAAAGTAAATTATATATATAAACATTGGTCAGTCTTCAACTTCTGAGTGAAATTTTAAGATTAACTTGGATATCGGCAAGATCCTGGTAGGTC

GTTGCGATGGTAAATATTGGTCAAACATAAAAGTTATGCTTTCTTAGTACCAAAATAAATCAAATAAAGTAAATTTGGAAACTAAATCAACTTGTTATTGCAACATACGGTTTGTCATCCTCCGGGTTTTTGGCAGCGCCCAGGATGCGCAGCCTGGCCTCGGCGTACTCCTGTTCTCGCTGCTTGAGCGTCTTGATGGGCTGCTTCGGTCGCATTCCGTTAATGTCCCGCCGCTCCTCCGCCTGGGCGGGCCGCCGCAGTATCTTGACGGTCTGGTTGCTGGTGGGCGTGCAATAGGTGGCCGCATCGTAGTCATTGGTCGTCTTGTGCAGCACCATCATGACGGGCTGCTGCTGTTGCTCCTGCTGGGTAGATGAGTCAACCTCTGCTGGCGGTACTTGGGGCTTCCGGGCAATGGTGATCTGCCGTGGCGGGGGATTCCCGCCCCCGTTTCCACCACCGCCGCCACCATCGCCGCTGCTCAGGATTTGTGAGGACTCCTGTACGCTCTGCGGCCGTTGGAGCAATTTCATTTTGTTGACAGGTGGGTCGCTCGTCTGCAGCTTTGTTTGCAGAGTCATAGAGAGCTGGGCAAGAAAAAATAAAAAAAAAGGGGGGGGGGTGTTCCATTGGTTATCAGGTATGATGCTCAATCACATCGCGTGGCGCACTCACCCCATCCTCGTCGATTTCCTCCCAGTTGTCTAACACATCCTCGCCATGGGACATCGTTCGACAAAGCACGTTGCTCGACTGCTGCTAGAATATATACTTCGGGTTTCGGTTGCGTTTTCGCTCACTTTCTTCTCTTGCGCCTGCTGCTGCAGCTCGCACTCTTACTGATACATGATTCCTTCGCACTTTTATCAGACATAAGTTATGCGTATGTTGGCAAGCACACTTTTTTCACGAAATTCGCACCCAAGAACCCTACTGAAAAAACAAAAAACAATACGCAACAATTTGGCCTGCTGCTAATGTCGTTCTCGTTGTTGTTGTTGTTGCTTTCCGTGGGCTAGTCTT

 

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