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CRIMIC CR00854

Gene CG31751

CG31751

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:18680181..18680202	36F11	FBti0199649	79321

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGGAGCAGTGGAACAATGTGGAGCT	ATGGCCTGGCTGAATAGAGCAGATGT

Images

Construct Design Sequences

sgRNA sequence	AACTACAACGTTGTCTTATTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAGATGGCGATCTCCTCAA	TCACAAATCAGTGACGGTGA	GGTTCTTCCCTGGAATGTCTT	ATGTGCATCCTATCCGGCTG

 

Amplicon 5'

Amplicon 3'

GCAGATGGCGATCTCCTCAAGCCAGGATCGGACGTGCGCCCAAAAGTGGAGCCCGAGGACGTGGAGAGCCTGCTGCGTCGCCTCTACGGGATCACGATAAGCGAGGTTAAGGAAATCGTCGCCTACGACGATCGCAACTTCTTTGTCAAGGAGGATAGGTAAGCAAAGCGATGACAGTATCTCGCTGACACCCTTTCCCAATCCCGCACCACATCCGGAGGTTTATCTCGTGTATGGGAGTTCAGCGTAAGCATCTCATACAGCTGCAGTCAGATTATTAGCACAGGCAATTTCAAACATCATAATATATCATTTAGATCAGGATTAGTCTTCTATATAGTAAACACAAGAATTCGTTGCTTGAAATAATATCTCCTTTAAAGCATCAACACAACATATTTTCTGTGCTTTGACATGACTGCAATTTTAGCTGCTGTTCGTAGTACGACTGTGCAACACGAAAACAAATAATAATGAGCCGGAGAAATGCGGCGGTATGTAGCATCTATTTATACCCAGTTTAATTAAAGTTGTTAGTCGCGGCAGTTGAGTGTGACGCAGTCGACAGTCACCTTGCCAGCATCGCAATTGCTATCTTGATGGATTCATGGCATTCAGCACTGATGTAGTCAGTTCAGCTCTTCACTTTAATAAATTAGATAAATCTAGAGAACAAAAAGCTTACAATCTAGAAGAGCCATCTATTTGAGGACAGGTTTCGTCTGGAAGCCAAACATGCTGAGCTTGGTTTGATTCTGGCTCACGGCACCCAGTTTTCCATTCGTCCTAGATTTAGGAGTGCCCTTCTTGGCAGTGGAGGGTTTACTCAGCCCCACTCTTCGTTTGCTGGTTGGCAAAAAGTTCTGGCGTTGCTGGCTGGAGGATGAGGTAGATGATGCTCTCTGATCCGAGCTACAGCTGTCGTCCGATAGCAGAATGATGGCATCATTATTGTTCCTGTCGGATTCCTGAGTGGGTTCCGGAGAGTTTTCCAGAAGTAAATCCAGTGACTTAATGCGAATAGTTGTTGAATTGGTATTGCCTTCAGTTGGTATTTGGGGTTGAGAGTTGTTGAGATCTTCTTGGGATTCTAAAGGCGAAGGAACACCTTTATCTTGTGATTCCAAAAGAGAAGAGGCTGTGGTATCTGTATCTGTTTCACCGTCACTGATTTGTGA

GGTTCTTCCCTGGAATGTCTTTACATACTAGTCTTAGTTTCTTATGGCCTGAGTGAGAGCTCAGCTTCTGCTCATCCATATCGTCATTTTCCGTATCTCTTAAGTGCTCCCTTTGAAGCCTTGTCTGTTCATCCTGCGTGGCGAAGAAGCGACTTCGGATCTGAACTTGTTCCTTGGCGAATATGCTTCTTTTAAGCGAATCAACCTTTTTTACACCAGCTTCTCCATCCAACGGACTTGCCTTTTTCGGACTAAGCAAACGCAGCAAAGAGGCATTCTCACAAACTACTGGGGATCGCTGATTAGCTTCTTCTCCAGTCCTTTCCTTGGCAAATGGATTGTGCCGACTTTTGTGCACCGGCGACGGAGGAGGTGTACGTTCTTGATCTACAGAATCTTCCCTGCTCGGACTTCTCCTCCTTTTGGCTTTGAAACTGTACATGTTAAACAACTCGGCCTCCGTTTGTTTAGCCTGTTCCAGTCGCTGATTAGCTTCGATTTCCTCGTTTAGAGTTTTGCCCACAAAATCCACTTTTTTGAAGAACAAGGCACACGGATTTTCTTTCTTCGGCGTGTGAGTGTTTTCGCTTTGAAAATTCGTTTGCCAAATACTTTTGTGCTTGGATCGTTTCACGTTCTTCGGCGTTGGCCATGCCTTTTCCGGTGTCCAAGAGTCCAGTCGCTTCATAGAGAAGGGGTTCAAGTTGCCTAGGGCCAAGTGCAAAGCCTGCTCGCTATCCTCCAGCAAGGTGCCAGCATTGCTGCAGTAGCGCTCATCAGTTTCATAATCTTCGAGGGCACACAACCGCTGCATGCGACGCTCTAGCGGGTTGTAGATAAACATGTGCCTGAAGGTGGCCTCCGCTTTCATGAAGTTTTCAATATAGTCGTCATCTACCTGGATTAAAGGATTATTAGTCTTACAATTCAGACCTAACCGCAAGTTAACTTACCTCAAGATTGCGCATATTGAGGTAACTTGGAATCTTTTTTAATGCTATTCGCATGTCTTCCTGTTCCGTTTTTAGTATAAATTTGCACGCCTTGGCCAGTCCGATGCCAGGCAGTGAGTCCAGGTAATCACAGCCGGATAGGATGCACAT

 

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