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CRIMIC CR00855

Gene RpL30

CG10652

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:19008576..19008576	37B9	FBti0200706	81149

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTTCGCCGTGCGGAACTATTTATTC	GGGAAAGTCGTCCCTTATCAATGCAC

Images

Construct Design Sequences

sgRNA sequence	TGTTCCAAGCGCCTTTATATCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAAGCTTTGGAAGGCCCTCG	CTCATCTGTTCCAAGCGCCT	AAGGCGCTTGGAACAGATGA	ACGGCGTTACCAATCGTCAT

 

Amplicon 5'

Amplicon 3'

TAAGCTTTGGAAGGCCCTCGAGGACGTCCACCTCAAGGAGGAAATTTCGGAGTTGCCTTCGGGTCTGCAGAGCATCATATCTGAGGGCGGGACCAACTTTAGCGTAGGCCAACGCCAGTTAGTCTGCTTGGCAAGGGCCATTCTGCGCGAGAATAGAATCCTAGTTATGGACGAGGCAACGGCAAATGTGGATCCTCAGACGGACGCCCTGATTCAGGCCACCATTCGAAACAAATTCAAGGACTGCACAGTACTCACGATAGCTCATCGTCTAAACACCATTATGGACTCCGACAAAGTTTTGGTGATGGATGCTGGTCACGTCGTCGAGTTCGGTTCTCCATATGAGCTGTTGACCGCGTCAAAGGCTAAAGTGTTCCATGGAATGGTTATGCAGACGGGAAAGGCCAGCTTTGATCATCTACTGAAAGTTGCAGAGAATGTAAGTACTAAAGTCGAAAATTTCATTAATAAACTAACTTTTCCTTCTATTTTAGACCAAACAAAACCATATTTGAAGCTAACATTTTTATCATATTAATGTGTTTGTCTACGTAATGTATATTGGGCCCAATTGTAATGGAAAGGGAATTTCGTGGTCATAGATTCAGTTTTATAGAATACAAAACGTTTGTACTTAAGGGAGTAAAGCAAAATGACAATGTTTTCTTTTAAATAAAATGCTACTTAAACAACAAGAATCACGTGCCAAATCTTTTGATGATAATCCCAACATTGCTTTCACTGAATTTACCAAAACAATAGTGTTTTTATTTTAAACTTTATCTCTGCTCATACAAAACTTTATTGTGTCGTCTTGTTCGATTAAAAATAAATGTTTAGGCCGTCTCCAGCGAGCGGATGATGTCCGAATCTCCAGGATCGGTGATGGACAGGGTGCACACGCGGAAGTATTTACCACAGGCGGTGCCCAGCTCGATGTTGGTGCCGCTGTAGTGCTGGACTTCAGTCTTGGCCAGCATAGCGTAGTACTCGATCTCGGACTTCCTGCAATGTGAAGAGCACATGTTAGTACCGATATAAAGGCGCTTGGAACAGATGAG

AAGGCGCTTGGAACAGATGAGCCATTGGTTCGAGCGTCAGAAAGAGTCTCTAGTATAAAAAGGATTCGTCAAGGCATTCGGAATCAATAGGATCATCATTATGGGCAGTAGACTACAGCTAGGTGTTTATATGCCGGCTTCTTACCCCAAGTGATGTGCATGCCCGTTGTTGGAGGAACCATTTGGAAGCGCCGTAGACTAAAGCGAACCAGTCCCGAAGCTTGAGATTGATTGATTGCACTGCTCCACTACTCGGAATCATACTTCTCAGTCAGCTTTAAGGGCAGACTCCATATGGTTCCATCAAAAACGTGGTCACTTGGGCACAGTGGAAACCTACTGCTTAGCAGCAGACGCATGGGCATCTCACCTCAGGGCGGGCGTGTTGCTGGCGATGAGCACCAGTTTGGCCTTGCCCTGGCGCAGGGTCTTCAAGGTCTGCTTGTAGCCCAGGCAGTATTTGCCGGACTTCATCACCAGCGCCAGACGGGCGTTGGTGCTCTCCAGAGCCTTCTTTTGTTTCTTAACGGCCACCATGTTGATAGTTTGGGTTAAAGCACTGCAGGCGATAGAAATTAGTTAGTCGAGTGATTCTGTGGAGCCGGATGAAAAATTAAAACACCCACCTTCGTCGGCTGACAATGGCAAAAGAAAGAGGAAGAACAGGCGGAAATGGGTACCACGTTTGTGTTGGTGTGACCGACGGGGGGATGCGAGAAAATACCACCAATTAATACAAATAGGGTTAAAATACCAATTAGCGTGTACACAATCCACAATTAGCGTGGACACGCACCGAAACTATCTTTTAGCCAGTACAACCTCCAAAGTTATGACGATTGGTAACGCCGT

 

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