• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00856

Gene Prp19

CG5519

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:18447837..18447839	55C9	FBti0199650	79322

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTTCGCCATTGCTTGGACGACTTCT	GAAACCCAAAGCTTTGACGCAGGTTC

Images

Construct Design Sequences

sgRNA sequence	CTCCGGTTCCACATGGGTCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGGCGATAAGGATGCCAGG	GTTCCACATGGGTCTTGGGT	GGAGCCTCCAAAGCCCATAA	TCGGTTTGCTCCTCCTCAAC

 

Amplicon 5'

Amplicon 3'

ATGGCGATAAGGATGCCAGGATAACGGCGGCCATAGCCAGCAACATATGGGCGGCCTACGAGAAGCATGGACGCAACGCCTTTCGCGAAGGTCGCCTCACTTTCGTGCTCATCGATTGTGAAAACGGTCATGTGGCCATCACACAGGTGGGTGATCCTCTTTTTTTTAGCAGGGATTCGTTCTATAAAATCTTAACTATATTCCTTTTTACAGGTTGCCAGTGTCCTTTTGTGCCTGTACGCCAAGCAAACGGTGGGATTGGGTCTTCTGAAGCAAAAGGCCATGTCACTGGCCTCCTATTTGGAGCGACCGCTTAAGCAGATCTCGGCCTCATAAGGATAAAACAATGATGTACCAAGTTCCGAAATGAATAAGTTAATAAGTGTATGCATCAAAAGTGAATCAAATGAATATGAATAGTCTGCGGAAAAGTTTAAACGATAATATATTAGTACTTGAAATTATGAATCTTTAAGGATTTTGTTAGTATTTAAGGAAAAACTTGTCTGCTGGTTTCCAAAAGTAACCCAAAGATATTTCGTATTCTGAATTATTTGGATAGAGGGTCAGGTTTATTATTCTGCAAGATTTGTTCAAGAAAACACACAATCATTAGGGAATATTGTATAAGAAAATTTTGGACTTAATTACTAAGTGTGGACTTATTCGATGGCGTAGAGCTTCAGGGTGCGGTCCATGCTGGTGGATGCCAGATACTGGGCGTGCTTGCCGAATCTCACGCCCGTTGCCAGGGCCGTGTGGTCGTTGAATACCTTCAGTTCCTGCCACTGCTTGCACAGGTAAACCCTGTAAATAAAACGAAGCAACCGTCAGAATTTGTATTTGTACCGTGGCAACCAAATAAACAAGTTAACTAAGGGAATCTTCTTTGGCGAATTTAACTTAACAATGCGAAAGTTTAAAAGAAAAGTTTACCGCGAATATGAAAGTAAGTATTATATTCTAATATGCGAAGACTTAAAAATGCATGCTGCTCTTCAAGATTTCGTGCGACCCAAGACCCATGTGGAAC

GGAGCCTCCAAAGCCCATAAACTTATCGATTGAGGACTTCAATGCGGTGCGAAATAATTAATTTTTATATTTCCAAAGTTAAGTTTTATTAATATTCCTCAGCGCGGAGAGATCGCCAATCCCCGTGCTCCTTGTGTGGTCACCACCATCGATCCCGATTATATTAAGGACGCCAGTGCGCAACGTTTGGCCAGAAAATTTCGCATGAAAACGTAAGGAATCAAATGAATTATGTGCTGGTGCAATTTCACAGCTTTTTCAGGGACATCCTACTCTTGAGGGAGATTACCCGCAATCGCTTTATGTACTATGCTACGCAAGAACTTTTTTTGGACCGAAAACGCGAGAAGGAATACCAAGACAATGTATACGAAAAGGCCGAGGAGTTTCACAAGTTTGCAGAGGAGTCGCTCAAGAAAATGGAAGCGGAGGAGTTTAAGAAAATGTTAGAGGCCCAAGAAAAGCTGAAGACACTGAAGGAAAGCAAACTGAGCGAAGAGCTGAACGAGGTGAAGCTGGAGCATCAGCGAGTGCTGATGGAAGTGCAGGAGGTCTACGGTCGCTTTCAGTATTTACTCAAACTGATCAGCTACTTTGATGACACAGTTGAGGAGGAGCAAACCGA

 

---

Search Again