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CRIMIC CR00860

Gene antr

CG30488

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:12847387..12847410	49D4	FBti0199652	79324

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAGTTTGCCTTGAGGCGTTGTGACT	CAATGATCATTGACCAGGGCACATCG

Images

Construct Design Sequences

sgRNA sequence	TCCTATTCTTGAGATATAGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGATCGCAGTAGTCGAAGG	ACCAGCTTTTGAGAAGGCC	GCGTTTTACGTCACTTACCCT	CAAGTGCTCATCCGGGAAGT

 

Amplicon 5'

Amplicon 3'

GGGATCGCAGTAGTCGAAGGCCTGGAGCTGCTGGCCCAGTAGCATCAGCAGCAGCAACAGCGGCGGCCACTTGGTGTCCAACATCTCGACGAGGCAGCCAGTTTAGCCAGCTCCGGCCACCCGAGGCTGCCTCTTATAGAGTTCGAAGGGGCCAAATCGATTATCGGTCAAGCGGAGCTATCAATTGCGACCATTTACTGACCACCAATCAGTAAAGCAATTGGTTAGGCCCACTATTAGGCACACTTTTGTACACAGAAAAAAATGGATAAGTTCTGATCAAATTTTTCTAGCATTTCGCATGACTTAAATCCGTTATAATAATTTGCTGTCATCGATTTTAAAGGTTTTTATTTCATGAATAGTATTTATGTGAATATTAAGTGCATAAGCACTTATTTTATAAGTGTACAATTATATAACACCCTAAATGTCCACTTGTATTTGGTCGCTGGACGGCATATTCGATTCCACCACCATGCTGTTGGCATCGACGTACTCATCCTCGCTGCAGAGGAACTGGTACATCTGGCTCGGTCCAGTGGCGCACTTCCCGGCAGGAATCTGGCCCTCTTCGTACGGAACCAGGTTGTTCACACTGGCCCGCGAAAAGTGGCAGAGCAGGATAATATTTGACTCCTTCTCGTCCCTACCTTTCACACGAAGGCCGCATCCCATACGAGAACCATGGTCCTGAATCAGGGGCATATAGTGGCCCACACAGGTTCTACAGTAAAAATAAATATGGTCTTAGTAGTTAAATATTGCAATTTCAAAAATGTAATATTAAACCCACTAACATGTGTTCGAAACGAAGTATTTAACCTAATACAATAATTTGTAAAAGATTTTAAAAGGCCTTCTCAAAAGCTGGT

GCGTTTTACGTCACTTACCCTTCTCTTACTGGCACTAATTTCTGACTGTTCATCATGCATCCCATGACATCCAGAAACAACTCCGGCAGCAATTTATCTAAAATCGCACTTTTAAGGCTCTTGGTCCGTCCCATCTTGCTACGGATTTCGGTGGTTGCCACATAGTGATACTTGTCGGTATTGGCGCAAAACTTTCCCGTCTCATCGCATTGGCGAACCTGTCTATCCGCCAGCCTCTGCAGTTCAAAATCCCAGCCCATCGTCGGCATGCGAGCGGCTACCGAATAGTTGCCCACTCCACTGGCCACATAGTTGCGCAACATGTTGATCCTGCTGAGGATGCCAGTCTTCAAGGCTCCATTAACGTTCAGGAACAGATTGTTTTTCCCGCATTGCTCACCTACTGCCTGCAGATGAGGTGGTTAAATCACTACAATCTCGAGTAATGATAGCTACTACCTTAGGTTGAAAGCAGCCCACATGAATTTCCGAGTTCATGCAGAGATTGGGCTTACAATGGGGATCATCTTCTGGTATCAAGCTCGCAGTTAGCGGTAACAAAAAGAGGTACAGATACCACATCTTCATCTCGTGGCAAAAGTAATTTAACTAACGTGTAGAGCTATCTTATGACCTAGATTTATTAAATGCAGCATGAACGAATCAATTAAGCTCGCCCAATTGCAGGGCATTGGGATTAACATGCTCCTCGGGTGAGCAGAGCCCCTCATATATGGAGTGGGTTCCCGCATAGCAGCGACTTCCCGGATGAGCACTTG

 

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