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CRIMIC CR00862

Gene CG30349

CG30349

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:8910125..8910125	44F6	FBti0199653	79325

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACCAAACTGAGTGGCGGCAAGTTGT	GATCTTCGTTCAGGGCATCATTTGGG

Images

Construct Design Sequences

sgRNA sequence	ATTATTGAGTCAGACCCGCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGTTGACCTTGGATCGGCT	TATTGAGTCAGACCCGCGTG	CAATAATGGCCACCGCTTCG	CAGTAGCCGCCGCTTATGTA

 

Amplicon 5'

Amplicon 3'

CAGTTGACCTTGGATCGGCTACCGCTCGATTATGTCAATCCCCTGGTCAACGAACTTTCCATCCTGCTGCAAGGAAAGCACGCGAGGTGAGTGTTTTCCGTAGAGCCCTATTGAGTCTTTTTCTAAATACGTTCTTTTACAGTGTCCGCTCCGCCCTGCGTTGGCTGCACGCACTGACCTCCACCCACACTAGTGTCTTGATGTCCGAGGACAAGGAGGCTTTGCGCGAAAAACTAGCCGTTTGCCTCGGCGTGGCGGAGCAGCGCATGCACTGTCTATCGGAAGCTTTGCAGTAAGTAAAATTGGGGAATTGAAAGACACAAAACAAAGGTTTTAATAGGGAAATCAAGTTCAAGATCCCGATTTCTTTGAATTTTTCGGCTTGATATTATTCTTGGGCTTTTCAATGGTGCCGTGGCGACACTCCGAGATCGCAGCGAAGAAGGTCAGCTTGGTGTGCAGAATGGTGTCGATGTAGAATCGCTGAAAAGTGAACGAGTTGAGGCATCGTTCGCCCAGGCCGTAGTAGAGCGTGTTGTAACCCTCGAAGCGGTAGGTGATCATCACGGGATAATGCTTCAATAGAATCTTCGACAAGGAAATGATGAAACGCACCTTGGCGACGGCCAGAGGATTCGGACAACGGGGTGTTGGCCGAGAGAAATGGGCATCCACAAAGAGAATGGTTCCCTTTTTGGCCACCTCGTGTCGAATCGGATTGCTGATCACCACACACTTCGTGTCCGGCGAAAGTTCCCAGCGACTGACAAAATCCCTAATGTCGGCAATGGCATTGGAGTATTCAAAGTCCCCGATGGTCAGCATCTTGCGCGCCAAGCCACTGGATACGGAGTCCGCCGAAATGCCAAGGCCACCCGTGTAATGATTCGTGATCGAGGGATCATCCTCTCCGTTGGCCACAATGGTGTCGGTGAACACTGAAGAGATCAGGCTGATGGAGGACATCTTTTGCTTTTTCGGAAAGTTGTAGATAAAAAGACCACGCGGGTCTGACTCAATA

CAATAATGGCCACCGCTTCGCAGAGTGTGCCTTCGATCAGGTCGATGATGCACTGCATATCAGCGTCATCGCAGGCCGGCTTGTTGCACTGGTTGACTAAGCTGGATATGCTGGGTATTATGGCAATGGGTAGGGCACCGCCTGGTCCCAATGCTGGCACGCCACTGCCTGGACTCGGGGGTTTCTTGTTGTGGGGGCTGGAGAAGTAGTCCATACAGCTAGCGCCATCATTGCACAAAAGATCCATTTTCGAAATCTGGCCACGACAAAATTTAGTAATGCGTGTACTCCTATTATTGTTATATTGGCAATATTGTTATAACACCTTTTCAATGCCTTCTTTATTTTTAGAATAACCGGGCGAGTCAACCTGATAATTAACCAGATGAAACGCAACGCGGACAATCATCTCAACGATAACAAAGCCCTGATCCTGGACGAAGATCCAGGTAAGTTATCAAGTGGTTTTATTCGCACAAGGCTCGAGTCTTAATTCGCTTGCTTGCAGATGAGCCCGATCCCGAAGCAGAAACGAACTGGAGCGATCTGGAGGAGGATCAGCCAGGCAGTCCGAAGAGCCCATTGGAAGACGAGCTGGTGGCCGACGACGACCTGGCGACCGACGATGGTAACGCCGACGCAGACACAGACTCTGACGTATCCATGGATTAGACAGTCTACCCGTAATTTAAGCTTAAATGCAATTCCCTTGTTGTTGCGCCGCGAGAAAATAAATTAGCATTTTTTTCCAAACACAGTCTTTTACATCGAATTCATAAATTTTTATTTACGGTTTTAAACTACGTGCAACAACGAATAGTGAGAGCCAATTGTTGGTCTAATTCCGACACGAGATTTCATCGATACGCAAGCAAACAAACATATCCAGTTCATAAATACAGTTGAATAATGTGCCAATAACAGGGGGCTTGTAGCTCGAGTATACATAAGCGGCGGCTACTG

 

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