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CRIMIC CR00863

Gene pelo

CG3959

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:9703500..9703530	30C5	FBti0199654	79326

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGTGAGAGTGCTATGGCAATTGGTG	TGGCCAGCTGCGACAAAAAAACACCA

Images

Construct Design Sequences

sgRNA sequence	CAGCTCGGAGATGGTGTAGTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTTGTTGGCCCCATAGCGA	GGCCATCCCATCCATCAGTG	TGGTGAGCATCTTGGATCCG	CTGTGTGGAAGCGAGAGTGT

 

Amplicon 5'

Amplicon 3'

ATTTGTTGGCCCCATAGCGACGTGCTCATGATCAAGGTTGCCCCAATGTCTCCTCACCATTTTTGGCTGCCAGCCTGTTTAGTTCTGTTCTGTTCTATTCACTACTCTTCTCCGCAATTTGTTTACACTTTAATGGGCCGATGAAATTCCATTCGAATATGTTTTTGTATGCTAACTGCGTGGCTGGCAATTAAATGAAAATTAAGTGCTATAGCTTTTTCTTTACTTAACATTTAATGTACTTAATGATCTAGGCAAAAGGTGTTTTATTTTAGCACTTTTAGTCAGTCCGCTTAGGTTGCTTCCCCCATAAGTATTCTTCCTCCTTCTTTTTAATCTCAGAAATTGTTTAAATCGCAGCTCGCCGCTATCGTTGTCGTCGTCGTCGTCGTCGTTGTTGCTCCGGACTGGGCTTTATCTCGACATTTTTGATTGCTGCTGATAGTTTGGCCATTGTGCAAATAGAAACAAGATGGGGAAATGTTGTTATGAAATGCTTTGTTTAAGTTTTCCATTTGCCTTACCGCCTTCGCTGCTGTTCATTGCACTGATTCTTTCTCAATTTCTGCTGGTATACGGGATCCTCTTCGAAATCTTCTCTGCTCCTCATCGTCAAGCGCTTGAAACAATCGTCCAACGTTTCCCTCAGTGGCAGCAGGCGATGTGGTGATGGCGGAGAACTGGGCATCTTTGTGTAGTCCAGCTTCTTGAGGGACTCGCACGCGGTCGAATCCGACCACTGATGGATGGGATGGCC

TGGTGAGCATCTTGGATCCGCCTGATTTGACTCGATTTAATTCGACGGTGTTAAGTGGCCTGCACTTAAATAGGAACTGGAGTGGAAAGATATGTGCGTTAAGTAGTACAGTCAAGTGGAGTTGAGGTTCGGACAGATAATTCAACGATTCGAGTTTCTTTGCTTTCGTTTTATGATCATCAAATCGATAAATATATAAAAATCGACATATATTTGACTGGAATGTGATCAAGTTCCTCAAAATGTACTACACATATTTCGAATTAAAGTCACGTAAATGTATTTATTTTGAAATCATTTTATCATAATATGTGAATATTTATTTAAGTCACATAAAATGAAATCATGCAGTTCAAATTTTGTGACTACTATAAGCTGGAATTTTGCCGGCTACAACAAAAGTATTATCAGCAAATGATTGTGGAAAATATGTAGAGCATTTCTATGAATGTTTGTGATAAGCCAAGATAACTTTATAGTTAACAATATTGTTAAAAATGGTGTTTAGCTTTCTGAGACTTTGTTTCGTCCCTCACCCATTTAATCCGTATCCGTAAATATAAACGTAAATGCTCATGGACGAATTCCAAGCTGAATTTGCGTGCTCTGTTTCGGGTCTTATTTTATAGAGCTGTTTTTGCCAGACGAGTTTCGATTAGCAAATGACGGGGACTGAACTAAACTGTGATGTAAATAAGCTGGGTACAGTATAAGCTTTATATAACCGTGTGGAAATGATTCCCTCAGTGAGCCAATTCTTGTGCTAATTATTAGAACCCGCTGTGGGCGAAAGAAGATTGCGATTGAGGGAGCAGGAACGGCAGCGGGAGAATAGCAATAAACAAGAAAATAAAGAATTTTAATTTATTTAATCACTCCGTTTATGGATCACTTGGCTGGCAACTTTTTGAAGCGGACACTCTCGCTTCCACACAG

 

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