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CRIMIC CR00864

Gene CG4038

CG4038

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:21096977..21096977		FBti0200708	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTGGCATCCGGACTACTCAGCTTCT	GGAATTCGGATGAGTGCGATTCTCTC

Images

Construct Design Sequences

sgRNA sequence	CTAAGCTAGCCTGTGAACTAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTGGCTTGAAGCTGTTGGC	AGCTAGCCTGTGAACTAAGGC	TCACAGGCTAGCTTAGTTTGG	TCGTGTGCTACTTTCGCGAT

 

Amplicon 5'

Amplicon 3'

TTTGGCTTGAAGCTGTTGGCGTAGACGTTGTCCGACAGCTTGATGGACACGCTGTAGTCCCGAACTGTTCCAAAGATTTCGTCGATTTTGCCTACTTGCTCCTTGTTTTCAAGGAAAATGGGCGCGTTGAAGTAGGGAACATCCTGAATATCAACTTTGCACACAAGGTCGTTTTGGCAGGAGTAAACATAGTTGCCCAAGGGGATGACTCTCTCTGGTGGACCTACAATTAAAAAAATAAGATATATTATTAAAGTGACAAAATGGAAATGAGTATTTGGCAATTTGCCTGAACATAGCAAGCATTTAAGACAGCGTATACCTATAGGAGACTTCCAGATGTGTTTTTAGCTAATATATTTATATATATAGCCCATGATTATAGCCTACAACGGCTTGGCTATTCAAAATTTGAGAGAACAACGTGGTTATTGTAATTCTATTCGCACAATCTGTATTAACTGCTTGTTTTAAGTGACGTAGTTAGCCTTAGTTCACAGGCTAGCT

TCACAGGCTAGCTTAGTTTGGATGATTAGTTATAGGAATAGTTGGCGACTTTCATTCACTTACCAGTGTCGAAGGCTCCACGGCCGCCACCACGTCCACCTCCACCTCGACCGAATCCGCCGCCACCTCCGCGTCCTCCGCCACCTCCTCGTCCACCTCCACCTCCACCAAATCCTCGTCCTCCTCCGCCTCCGCCTCCACCGAAGCCTCTTCCGCCGCCGCCACCGCCGCCACGAGGTTTACCAAATCCCATTTTCAAATATTATTAAAATTGAACGCAAAAACACGTGCGCGTTCCGATCAACCAAAATATTTAGAGACGGAAGAAATTCAAATTTCGTTATCGACTTATCGATAGCCACGGCCATGTGTTTCAGTATATACGGTTGTATATTATACGGTCACACTAGGCACAAGGGGCGGATTTTTTGAAACTTCTATTTCAATAAAATTATTTAAAACTATTTTTAACAATCGACCTGATTATTAATTTCTTAGTCTTTTTTTTTAAAAACAGTTATTTTTTTATGCATATGAATAGTTTTAATGAAGATCGTTTAACTAAGACAAATTCCTGGTGCTTTTTTTATAAGCATTAAATATTCACTAAGAGAACATAAAACATTGAGTAAGAGAGAATCATCAAAAACATCATCGGATGCATTATAGATATATATATATATAGATATCTATAGCCGAACAGCTGTTGAGACATCTACCAAAAATATAGACAAGCAAAGTGAAAATGAATATTTGAATTTTCTCCAACTGCTGAGAGCAGTTGGATGCCAGCCGTGGACGAGGAAGCGAGAAATAGAAGCTAAATCTATTCGATCGTGTCGCGACGTCGAAAGAAGCAGATACATAAAATTCTATATGCAAATAGATAATATAGCAAAATAATTTCAAAAATTTTCGGACAAGTGCAGACTTTAAAAGTTTCAGAAAGTGCACAATACATACATATAATACGAAATTGCAATCGAAAGTGATATAATTACCCGAGGCATATTTTCATATGTGGAATATGAACGCCTCGTTGGGCAGTTAAAAATAGATTAATAGATGGCTAAATGACGAGCACGAGCACAAAGATAGATCGCGAAAGTAGCACACGA

 

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