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CRIMIC CR00865

Gene milt

CG43227

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:7053447..7053467	27D7	FBti0199655	79327

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGTGGAGTTCAAGCAGCGTTACGAA	ACTTCACTCGCAAAAGGCATCCTTCC

Images

Construct Design Sequences

sgRNA sequence	ACACAACAAGAATTATAAATCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAGCGGCAACTACATGGAC	AATCGGGCTTAGAAATAATGCG	CACTTTCAGTTGTCCTGCCA	TCGAGATACCTGGCCCTGAA

 

Amplicon 5'

Amplicon 3'

AGAGCGGCAACTACATGGACAGCGGCAACGTGTCGATGACGCAACTGGGAGCTATGTCGATGAGCAGCTCTTCGGGGCCGCGCATGGCTTCGATGGCGTATCCAGCGGGCTCCTACTATCGTGGCGGTAGCAATCAATCTCTGGGAGTTAAAACTCTGTCAAGCGAGAGTCTCAACTCCCAGTCGGATGACGGTTATCCAGCCCAGCCCTCTGGTGTGCCAGGAGCGCCCGGCGCCAAGGAGCTGGAGGCGGCTCTCAAGAGGCTGACGCCGGCTGAGGTCTTGGCTCGCCGTGCTATGTTGTCCTATGCTCCGGCTGGAACCTATAACTACGACGAACCAATGGGTCACGGTACAGGTAACGTTCGAAACTCGGATCTACCGCTGGGTGTGCGCACGCCGGACAGTATTATGTCCACCGGCTCGTCGGGAATGTCGGGCTCCACGAATCACATGTCCGCTTCGATGACGCATCAGTGGCGACTACCCGAGAAGCTGCAGATCATCAAACCCATGGAGGGATCGCAGACATTGCACCATTGGTCGCGCTTGGCCACGCCCACGCTCAGTGGACTGCTGGATGAGCGTCCCGGCGTAACGATCCGTGGTGGACGTGGCCTGGATGATCTGGGAATGCAGATATACACGCTGTCGGACGTAGAGGAGGATGTTAGCGATGATCTGCCCGGCAAACAGTTTGAAGCACCGGGCTGCACGTTCACCTACACCAATAGCATGGTCATGCATCCGGACGATGGATTCGTTAACGATCTGTCATTCCTCTCGCAGTCGCAGATGTCGTCCCGAATGGCCTCCACGTCGACATCAAGGCAACCCAGGTTAGTATTATTAAGGGTGGATAGAATCAGTATACTCGTTTCCTTCTTTTTTCTTTTTTACGCATTATTTCTAAGCCCGATT

CACTTTCAGTTGTCCTGCCACGCCTCGCGCTGGACTATCGCGCAAAAATTCCTGCTCCACATTTTCGGTGAACCTCGGACTCGCTGGAATGCTGAATGAGAGGGGCATCAAGGCGGTGACGCCCAGTGCCCTCAACACGCCCGCCGGACCTAACTTTTCGCCCACGGTGACGCCATGCAACAGCCCGGAGGGATCACCTCCTCGTGCCCAGTCGCCCGAGCCGCTCTTTGGTCTGCTCTCGTGTGGTGCGGATTTGATCCGGCGCAAAATCGTAGGCGATCAGCATCAGCAGCAGCAACAGAAGCAAAGGAGCAGCCTTAGCAAGCAGCAGCAGCAGAAGATCATGCTCTCGCACCTGGAAAGACGTGCCCTGCGATCGCTGAACCTAATTGAGAAGGTGGAGAGCATCGGACTGGAGAACATAATAAGCGCACAGAGAGGCCTTGGCTCAGGAATCGCGAACCGCAGCAGTTCGCCCCTGAGCAGTGGCAGCCTACAGAGTCTCCACACCAGCAGCAACAGCATTGTGGACGACATACACTTCGATCGGGCACAGATCAAGGGTGTCCTGCATCGAGGCCTGAAATCGCCAACGCCCGCAACAGCATCAACATCTGCAGCTGCGGCGGCTTCAGGGCCAGGTATCTCGA

 

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