• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00866

Gene Acn

CG10473

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:19047080..19047080	37B12	FBti0199656	79328

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGATGTTGAAAAGGACGCTGTCGAG	TACAAACGTCGGCCTAATTGGGAACG

Images

Construct Design Sequences

sgRNA sequence	TCACTGACTCCCTGGAGATTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTCCCCACTTCCACTGATG	ACTGACTCCCTGGAGATTAGGT	CAGGGAGTCAGTGAAATATGCCT	TCAGGCGGACTTGGTCTAGA

 

Amplicon 5'

Amplicon 3'

GGTCCCCACTTCCACTGATGATGACACCAAGCAGAGCGAGCCAGAACAAGAGGTAAAGGTTCCTTCTCCTTCCAGCAAGGATGAGGAACATCTAAAAACGACTAGTGATGTATTAGACATTCAGACAGAAGAGGTCGATGAAAGGGACGAAGAAGCCTCCGCTCCTGCGGAGCGCAGAGAAACCAAGAAGAGGCACAAGGAGCAGATAAAGGAACAGCAACCCGCAGAGGAGGAGGAACACAAGGAAAACCACAAGCAGATAGACGAAAACCCAGAACAAATTCACGATGAACCAGCAGATCTTTCGAACGACAAGCACGATCCATGTCTAAACAGCAGCGCAACCAACACAACCGGTGCTCCAGTTCTGCAGCAACAACGGTCGGTGCGGAAGCGAAAGTGGCTGACTAACAAAAAGTCTTCGGATCGTACCCCGCCCGTGCTTGCCATTTCGACGGATTCCCTGAAGAACATTATAGCCGATGTGGTGCAGCCAGTGCCCCTAAGCGATGTGCAACTGGAATCGTCCTCAGAGGAGGAAGGCCTGGTCACCAGCGATCGCGATTCAGAGCGATCTGCTTCACCGGCGCCCCCATCTGAAGCAAGCAAAGACCGCGATAACAAGGGCAATACCGCCAACACCTGCGACACCACCGCAGAGGCGTCGGCAGATAATGAAGGAGCAGGAGCCGCTCCTCCAGCAGCAGCAGACACGGTTTCAGCCCAGGTCAGCCTGAGCAAAGCCACAGTTTCCACGGCAGCACCACACATAGTCCGTGACCCTAGTCCTGCTCGAAACCGCGCCAGCCACGTGCTCTATATCACCAATTTGGTACGCCCCTTCACAGTGCTGCAGCTGAAAGGCCTGCTGGCGCGGACGGGAAAGATCGTTGAGGAAGATGGTTTCTGGATAGATCGGATCAAGTCTAAGTGCTATGTTGCCTATTCCACTGAAGAGTAAGTATTGTAGTTGCGCAAATAGAACCTAATCTCCAGGGAGTCAGT

CAGGGAGTCAGTGAAATATGCCTATAAAAAACTCACACTTGCAATTATTTTGCTTTTCAACCTTTAAGCGAGGCCATCGAAACCCGACATGCCCTGCACGGAGTTCGCTGGCCAGTGTCAAATCCCAAATGTTTGAATGTAGATTTTGGCAGTCGCACTGATATGGATCGGGCAATACTTTCAACGAAGGACGAGGCTCCGAGGTACGGGCAGGAAAACACCAGAGATAACCAGCAGTCGGGCAACGCTTGGTCCCGGTTGGAACCGTCTGACAAAAAAGTAATGGATTATTATCACAGCATAGAAATGCGGAGCTAACTTGTCTTTTTCCTTCAGCCTGCGCGACCTGTACGCGAATGGGATGTTGGCAAAAAGGAACCAAGCGACCACGACAAACACAGCAACGACAGGCGTCGGGACAGCAAGGATCGACTGGACTCGAGGTCTAGGGATGCGGAGCGAGCCGGACAGGAACGAAAGCGTTCTAGGGACAGAGAGGGGCGAGGACGCGATCGGGAGCGGGAGCGACTTGATCGCAATGCACATGCAAGGAGCCGCAGTGGATCGCCAGGTAAAGCCCGTGGGATACATTTTCCTGTCATGAGAAATTCAAAAACACCCCGTTTAAATAGCGTCCAAAACTAAGAAGGAGAATGAACCGCCTATCAGACTACTAGATGATCTATTTCGAAAAACCAAGGGAACACCTTGCATATACTGGCTGCCACTTACGCCGGAAGCGGTAAGTTGCTGAGATAAATAGTTCCAATCAACGAGAATCACTTCTAGACCAAGTCCGCCTGA

 

---

Search Again