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CRIMIC CR00867

Gene for

CG10033

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:3625601..3625609	24A2	FBti0199657	79329

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAAAGTTCCTCCGCCTACTCCACAT	GCCATCGGCGGACTCGCAAATAATAT

Images

Construct Design Sequences

sgRNA sequence	TCTGAAGAGATGCCGTCGATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAACTCACCCCTGGAGAGC	GACGGCATCTCTTCAGATCGA	CCGGCGGAGAAAGTTACCTTA	ACGTCTCAGTTTGGAGCAGG

 

Amplicon 5'

Amplicon 3'

GAAACTCACCCCTGGAGAGCCTTCTCTCCAAAGAAATCCCCTTTGCCCAGCATGCGAATGAACTTCTCCTCCTGCGTGTCCTGCTGCTTGATCGTCACTCGCACTTTTCCCTTGGATATGATGAAGAAGGTATCGCCTCGGGCGCCTTGGCGCACTATGTAGTCGCCACGCTGGTAGTGCGTCTCCTCCAAAACATCGGAGATTTTGATGAGCGTGTCTTCCGCCAGGTCTTTGAAGATGGGCACACTAGCGGTAGATAAGAAGACCAAAAATTGTTAGTTTCATTCGAGATAATCTGCGTTGCAGGCCAAAGAAAGTGCTGTAATTAGTGGCCGAGCCAAGTTCGATCTAGATAAGAGAATTCCATAGTAATGGGAATCTATTGTTGACCTACATCGATCTTCAAGCTGAAAGTCGCACCTGAACTCCTCCTCCCATTACTGGTTTCCCTCCGGCTGGAATTTTTTCATGTGCATCGCACACATCTTTTGTTGTTACACTTAACAAAAATATTAACAAGTTATCTTCCACATAGCCACCGTTTGTAGGTTTCAGTTTAATCTATCTAGCATTTTCGATTCTCTTCGATATTTGAGGAATATTTCTTTATCTTTTTCTCTCTCTGTAGAGCAGCGTAATCGAAGCTGAACTCCTCCCATTCTGTTTTTTCCCGCCGGCTAGCATTTTTTCAGTGAATGCTCACATGTATCTAGAGTCTATAGCTGCAGTTGCAGTTGCAGCTGCAGTATCTGTATCTAAATCTATGATCATCGTCATCATCAGCCCATTGCACTTTCATTTTCAATCTGCTTTTTCCGCTACTTCTCGAATGTGGAATAGGCAACGAGTGCATGGTCGAGGCCTCAATTATGGTTTTTAGCAGTTTTTACGGACGACCAATTAAACGCGATTAATTTGCCGGTCACTCCGGAGGCAGGAGCGACTGGAGATGGAAGTGAGACTGGATACAGCCGCGCAGACTTGGCCAAAAAACTGGTTGCCATGGCTGGCATTGGGAAAGAAATTTCTATGCGTTCGTTGGCCGCAGCCTGAAGTTTAGCTGAAGTGAGTTTTCATTTGAGTTTGAGCCATTGCACGTCATATGGACGCCCAACACCATGACGATGATGAGATGGCCATAATGCTGCATTCGATCTGAAGAGATGCCGTC

CCGGCGGAGAAAGTTACCTTACTTTATTCGCCATACCTTGCACGATTTGAACACACAAATCGACCGGGCTAAAAAAAAAAAAAATCCTCAAGCTAAGCGATTTTTACATTTGCCATTATAGAGTAATGACCATACACGAGCTGCACTGCGGAATTGATATAATTAAGTGTAAATGAACATTAATTACGCCCAAATTGCAAATAACCAGTCGGCACCATCTCGCCAATAAATCGCCACAGGCTCAGAGGCTCATTAAAATGCACATTAAATCGCCGATTGGCAATGGGGAAGTCTCTCCTGGTCTGGTCTTCGAGAGGAGTGGCCGAGAAGGCGGCGATGAAGGAGGTATTCCAAGGAGGAAAGCCACGGATCGGGGAGGGCTACTCACCTCTTGAGGAAATCGCTGTACTCCGCCTGCCGGATCAGGCCCGTTCGCATCATGATGGTCTGAAAGCACTGGCGCTCGATGGCCCACAGGTTGCACTCGGTGATCGCGGTGATGGTCGCCGTCCGCTGGCAGTTGTACAGGATCGCCAATTCGCCAAGGACCTTCGCTCCCGACAATGTGGAGAGGTACTTGCCCTCGCGGGAAACCTCCACGCGTCCATCTGGAGAAAATATATTAAAATTAATTGGGTAATCATAATCATAATTCTGTCCTATCCTATTGATATAAAAGCTACTATGCCTATCCTGACTTAAAACATAGAAATCCTTCCACCCCTTACCCTTCTCTGCTTCCTCCTCCTGCCCACCGCTGGATTGCTCCTTGAGATTTCCCGTCCTGCGTCGACGGGCATATGTGTGCACTGGAACGATGAGGCGCGGTCGTGGGATACAGAAACCCTCCCCGTTGAGATCCTTGGAGCTGCCATTACTGCTGGCAGCTGGTGAGATTTGTGGACTCTTCTGACGAACAGCCTTCTCCCCCTCCAGTTTGAGTCCCTCGATGGCCTGCTCCAAACTGAGACGT

 

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