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CRIMIC CR00868

Gene CG34124

CG34124

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:4961563..4961563	25B5	FBti0199658	79330

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATAACAGAAAACTCGCGCATGCGCCA	GAACTCGGCGTCCATTTTGACATGGA

Images

Construct Design Sequences

sgRNA sequence	CTCGTGTTGGCAGTGTGAAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGAAGAAGGCCAATCCCG	TTCTCGTGTTGGCAGTGTGA	CACACTGCCAACACGAGAAAT	CTCGAAGTAGGCCGGATCAC

 

Amplicon 5'

Amplicon 3'

ACTGAAGAAGGCCAATCCCGGCCTGCAAATTGACATAGAGTCAGCGCTGGCGGACTCGGAACCGGATGAGGAGGAGGAGCCGCTTCACATCCCGACCGTGCCCAACGCCATAATCTGGCTGCGTTACACCCTAAGAGACACAATTTGGGTGTCAATGGCGGGCTACGATGCTGGTTATATCTATGAGCTGGAGTTCAATGCGGCGGAGCCCACTCGAGCCACCATCATAGCGGAGGCGGATGACATCGAGATTCATTCGTATTGCATAATGTGAGTTTAAGCGCTAGAGATGTGGCATATCCTGGTATATCCATTTACTATCTCTTTTCCAGCGATGAATTCATCATCTTTGGTCTTCACAATGGACGTATTCGAATCAATCGCATCAATCCGGACAATTTCACCGACCTCTCCGAGTTCCGCATCTATCCCATGCACGACGGCCTGAAAGGCACCATTCCCAAGATCGACATTAGCTACAGTGGTGAGCACTTACTCACCATTGGCTACGATGGCAACGTGTTCCTGCACAAGTGGAACGGTCCGAAGATCGTTAGGAACAAGGTGAGGGATAAACTGCCGCCCCTGCCGGAGGGAGTTACCCAGATTCCGGACATAGAGCCGGAGGCTTGCTCCCTTGAACAGGAGAAAATCAACGCGGAGCTGCGGCGGCAACAGGAAGCAGCCGATGCACATGACCGTGATGTGCTCAGTAAGATCGGAGCGCTCCAGAACGTCTACTTTGACATTATCAGGATCAACGAGGAGCTACCTCCGGGTCTGAGGGCCAAGGATACCGACTTGCTGCTGGACGATCGTATCACCCGGCAAATTCGAAATGAGCTGCAGGCAGAGTTGGATGACGTTCGAGAGGATCTGGCCTACGATTTGGAATTTGCCCAAGTGGGACACACGAAGCTCTACAATCACTTTCTCAAGGAACTCGTCCAAGTCCCCTTCACAGTCGCTCCTTTGCGGTAAGTAAAAGGATTCCTATCATTCCAACAGATTTAACATTGTGATTTTAGTGCCAACGTGCCGGGTGTGTCCACATTTCGCCTGCAGGCTCTGGGCGAGGAGCATGCTCAGATCAAGCAGGATATAGAGGACCGCCTGAAGCGGGAGCATGATATGGGGTAAGTGGCGAAAATCAGGCTCCACCTCTTCACACTGCCAACACGAGAA

CACACTGCCAACACGAGAAATATATACAATACATACAAATCTTACTCTTACATTCAACACAAGCAGCCTTTACACGGAGGATCGAGTACTAGAGCAACCGGAAGTGTAAGCCATCCAAGCTACTTAGTTCCTAGTTTGATCACCTGTCCACCATAATTATTGATTACACCATCAATGCATATTACAAATCATAGATTAAACTTTAATGGCACTAGTTAAATACCTAATACCCAATGATTACAGACTGCATGACTATGTGGCGCCCGAGAGATCGGACGAATCCATTGGGGAACCCCCGCCGGAGTCGTTCTTCTTTGGCCGGGATCCGAAAAGCATTGAGCCGCGTTTCAGCAAGAAGATGATGCGACTCCTCACCCGCTATCGAAAACGCCAGATCTTCGAGGTGCGCCGCATCTTCGACTGGGACAAGCTGGAGCGCCAGAAGCCGGACCCCAATCGTAACCATCCCGACGACGATGCCCAGATCGAGGATGCCAAGCGCAACCTGGGTGACTTCAAGCTGAAAATCGGTTCGGACTACGAGCCCAAGTCATCGGAAACCTTGACCCAGAAGTACATAGAGGTGGTGGAGTGTCGTGAACAGTACTTTGCGATGGTGGACGCCTTCAATCAGATGGTGCTGGATCTGCGTGAACGCAAGGGTGAGCTGGACCAGTTTATCACCGCCAAGAGAAACCGCTTGGCAGTCATTCATAATTATCTGCCCGAGCCGGACCGTCAGCCCTTGGATCCGATTCAGGAAATTGACATGGACCTGGAGTACCCAGAACTTAATCTCATCGAGCACTATACGCCCGGCTGTGGAGTGGAGATCGACGACATCCTAACGCTGGAGCACAGTGTAGACCAGGTGGGTGAACTGAATGTTACTACTTTTAATTAAGCATGCATGCGTACATCTTCTATGTAACAAGGTGATAGCCAGGATGGCTCCAATGAGATCCACCGCCAACGTAAGCCTTTTCAGTTTGCACGGCTTGGATGTACCCGAGCTGCACGAGCACACCGCATTCACGATGCTACAGATCTCAAAGCTGAACAGAAGTCTTCCCAATGCGGAGCTGGACGAGGAGCTGCTAAAGCTGCCGCCCAAAAGTGATCCGGCCTACTTCGAG

 

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