CR00869

CRIMIC CR00869

Gene CG9536

CG9536

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:6476047..6476047	26D7	FBti0199659	79331

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGTACATAAAAGCGGATGAGAGACC	GTACGTCGTCCAGGTTAATAAGGGTC

Images

Construct Design Sequences

sgRNA sequence	ACTCTGCCCCTAGATTTAAATGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCATTTGCACCGATACCGAC	GCCCCTAGATTTAAATGGGCG	GGCAGAGTATGAAAAATTACCGC	GGTAACGGTCTGGTCACGTT

Amplicon 5'	Amplicon 3'
GCATTTGCACCGATACCGACGTCAGGCTGGCCGTAGATATCGTCCGCAGCGGCGTGGGCGTCTTGACCACGCCCGCCCGCAAACAGCAATTGTAAATGGGATTAACCAATACGCTGATAAACGGCTGTGATACGTTTGGGAAGAAGCTGACGAACGTGAAGCTCTCCGAGCTGTCGCCACGCCCATTGGGATGGTGTTGATAGAAGCGGAGATAGATCCAAGAAACCAGCGATCCGGAGGCGAACATAGCCGGATAAGTGCCATCTAGCAGGCCAATGGCCCACAGTATGATGGCCATGATGAGCACCGTGAGGGGAACGTTCCTGAGTAGTAAAGAGTACATTTGTATATTCAAGTGGCGGCTATGATTCAGGATTTACTTTGAGAAATACAATATAGTATCTTAAACAATGGTGTGTTTGTTATTATTTACAAAGTATCATTTCATAGAATTCTCGAGGGTATTTACTTGCGCGTTAGTAAACCAATATTTATTGAACTTTGACCCAAAGTTAAAGGGCTGATGAGCCAGTAAATTCTATTCAATTTATTGAGCTTGCGCCCATTTAAATCTAGGGGC	GGCAGAGTATGAAAAATTACCGCTCTTTGATTCTGTGAATGTATCTTTTTAGGTTTAAAGCCATATTTGGAAATAATCGAGAGATTTCTAAGACTAAACTTTCTAAAGCGAGCTCTTAGCTACCGTGTGACTCTGAAATGGAAACATATCAATCAATTATCTCACCTATTGGTCAGACGTCCGTAGCGCGTCTTGAAGATCAGGTGATCGGGCATTATCTGGCGTACGGCTACGCAGATGCCAGCCACGTATCCGGCCAGGCCGTGAATGTGCACCTCGAAGAGAATGGTCGGGTTCTTGGTGACCATGTAGTAGAACAAATAGTAGACAGTGGTCAGCAGCGAGACGCCAAAGTTGCTCAGCGCGAAGAACTTGAACATCTCCAGCTGGCCCCAGAGCGGTTCCAGCATCTTGCCGCACAGGCCGACGGTGACCACGTCGACGGCCACCTCCCACCAGTGCAGCTCGATGAAGCAGAACGTGAAGGCGGTCCATATCCAGAACTTGCCATTGGGCAGGATGTAACCGGGAGTGACGCTGAGCAGCAGGATGGCCGTCTCCGAGAAGGATAGCAGGTAGCCGAACGTGGTCACCACGCAGATGAGCGTTATGACCGGCGAGGTGTTGTGCAGCAGAGCATTGAGCTGTTGCCAGATGTACGGCCAGTTTCTGGCCAGTTGAGCTGACATGGCACCGATCGCTTCTCCTGGTGTTCGCACGCGTTTTTCGTTGGCCTCGCAGCTCCTGGGCGGCGGACTGGAACCGGGTGGTCTTGAATGCGCTGCGGTGCTTGTCTGCCTCGCAACTCAACACGTTCGAGGGGCCTTAAAGTGGTTCATTTGCTAATATTTACGAATTTGCTTCACAATTACAATAACAAGACTGTCATCGAAGTGGAAAGGCGTAGCTATCGTTTATCGATTGTTTCGCTACAACGTGACCAGACCGTTACC

Amplicon 5'

Amplicon 3'

GCATTTGCACCGATACCGACGTCAGGCTGGCCGTAGATATCGTCCGCAGCGGCGTGGGCGTCTTGACCACGCCCGCCCGCAAACAGCAATTGTAAATGGGATTAACCAATACGCTGATAAACGGCTGTGATACGTTTGGGAAGAAGCTGACGAACGTGAAGCTCTCCGAGCTGTCGCCACGCCCATTGGGATGGTGTTGATAGAAGCGGAGATAGATCCAAGAAACCAGCGATCCGGAGGCGAACATAGCCGGATAAGTGCCATCTAGCAGGCCAATGGCCCACAGTATGATGGCCATGATGAGCACCGTGAGGGGAACGTTCCTGAGTAGTAAAGAGTACATTTGTATATTCAAGTGGCGGCTATGATTCAGGATTTACTTTGAGAAATACAATATAGTATCTTAAACAATGGTGTGTTTGTTATTATTTACAAAGTATCATTTCATAGAATTCTCGAGGGTATTTACTTGCGCGTTAGTAAACCAATATTTATTGAACTTTGACCCAAAGTTAAAGGGCTGATGAGCCAGTAAATTCTATTCAATTTATTGAGCTTGCGCCCATTTAAATCTAGGGGC

GGCAGAGTATGAAAAATTACCGCTCTTTGATTCTGTGAATGTATCTTTTTAGGTTTAAAGCCATATTTGGAAATAATCGAGAGATTTCTAAGACTAAACTTTCTAAAGCGAGCTCTTAGCTACCGTGTGACTCTGAAATGGAAACATATCAATCAATTATCTCACCTATTGGTCAGACGTCCGTAGCGCGTCTTGAAGATCAGGTGATCGGGCATTATCTGGCGTACGGCTACGCAGATGCCAGCCACGTATCCGGCCAGGCCGTGAATGTGCACCTCGAAGAGAATGGTCGGGTTCTTGGTGACCATGTAGTAGAACAAATAGTAGACAGTGGTCAGCAGCGAGACGCCAAAGTTGCTCAGCGCGAAGAACTTGAACATCTCCAGCTGGCCCCAGAGCGGTTCCAGCATCTTGCCGCACAGGCCGACGGTGACCACGTCGACGGCCACCTCCCACCAGTGCAGCTCGATGAAGCAGAACGTGAAGGCGGTCCATATCCAGAACTTGCCATTGGGCAGGATGTAACCGGGAGTGACGCTGAGCAGCAGGATGGCCGTCTCCGAGAAGGATAGCAGGTAGCCGAACGTGGTCACCACGCAGATGAGCGTTATGACCGGCGAGGTGTTGTGCAGCAGAGCATTGAGCTGTTGCCAGATGTACGGCCAGTTTCTGGCCAGTTGAGCTGACATGGCACCGATCGCTTCTCCTGGTGTTCGCACGCGTTTTTCGTTGGCCTCGCAGCTCCTGGGCGGCGGACTGGAACCGGGTGGTCTTGAATGCGCTGCGGTGCTTGTCTGCCTCGCAACTCAACACGTTCGAGGGGCCTTAAAGTGGTTCATTTGCTAATATTTACGAATTTGCTTCACAATTACAATAACAAGACTGTCATCGAAGTGGAAAGGCGTAGCTATCGTTTATCGATTGTTTCGCTACAACGTGACCAGACCGTTACC

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