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CRIMIC CR00870

Gene x16

CG10203

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:6918130..6918135	27C4	FBti0199660	79332

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGGCACCTCTGTCGCCTGTTAAAA	TTCTTTGCTGGTGTTGGCTGATCCTC

Images

Construct Design Sequences

sgRNA sequence	GATCGACACAATGATTGTGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAAAGGTAGCCTCTTCGCCA	CAATGATTGTGCAGGCCCAG	GATCTTGTCGGGCGAGAACT	CTTTTACACGCGGCATACGG

 

Amplicon 5'

Amplicon 3'

CAAAGGTAGCCTCTTCGCCAAAGGCATCAACACGCGTGGCAAGCGACGCTTTGGCGGCCAGTGAACGCGACATTTTGCCCTTGTTCTTCTGGCTTGCCTGTCCCACCAACTGGGCGTGATAGATCAAACCGTACTTTGGAGTATCCTTCTTGGTCTTCAGCGCACGGAAGAGTGCTTTCTCGGCGCCCAGAATTTGTACAGTAGATGAGGGATGCTTGGCCAGGTTAATCAGCGAGCCAGCATGGGCAATCAGTCGAGCGCCGACGGTGTCGCCCACGAGCACTGTCAAATTCGGAGCCATGGCCATCATTCTGGCCTTCAAGTAGTCGTACAAGTGGGTGCGGTAATCGTTGATCGATATGATCTCGTCGCACAGACACTGAATGTTCAGCACATCCTCCTCGGAGATTTCGGTACCCATAGAGATCTCAGCTGCCTCCTTGACCTTTTCCTCCACATCTTCTGGCAGAATGTCGGACAGATCGCTTGTGGCCATATTATCCCTGGTGCCCACCAACTTGATGGTCTTCACGAAGGCAATGTTGTCGGTAATAATCTTGCCCAGCTCGGGAAAGTGCCAACCGTACCACTCGCGTGCACGCATCATGTAGTTGTTCAACTCCTTGTCCAGGTCATCCAGCAAGCACTGGGCCTGCACAATCATTG

GATCTTGTCGGGCGAGAACTTAAGCTTGTAGCGCGACAAGGAGTGGGCAAGACCCAGGGCCATGGCGGTCATCTCACGCTTGGGCAGACCACCAAGCAGACTGTCCGCCTGCTGGCGAATACAGCGCATCAGCTCCTGGACGCCAGTGTTGCAGACACACTGTACCGACAGTTTGTCCTTGATGGCTGTGCCCAGTTTGGCATCGGCAACCAAGAGCGAAGACTGCACGTCGTCAACGAGCAGCTTCTTGAGTGTCTTTTTCAGCGGCTTGGCTACCTTGCCCTCCACCGCCGCCGTTGCAGCGGCCAGCGCCTCTGTGGTGTCATTGAATTTCTCAAAGTGCTTCAGCTTCAGCAGCTTGTTGGCCTTCTCCGGAGTCTCAAACTCCTGGTACAGATTGTCCACCTGCTCCAGTTTCTTTTCGTCCAGCAGCTTGAAAATCGCGTAGCCCGCCGGCGTTTCGTAGAGCACAAACATCGCGTTACGCTCTGCTCATTTAGTTTCAATTTTAATTCAATTTAAAACCACCGAAACTCTCGGCAGCCAGCAGCAACTTAACCTTCTTTTCCGTATGCCGCGTGTAAAAG

 

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