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CRIMIC CR00871

Gene cdc14

CG7134

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:7805036..7805048	28C4	FBti0199661	79333

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACGGCGACTTTAACTGGATTGTGC	GGAGTCCGAAGATTTCCACATCCTTG

Images

Construct Design Sequences

sgRNA sequence	ACTTAGAAAATACGCGTAGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATCCGACGGTCTGCATACC	ACGCGTAGATGGCACTTCAA	CAGTCGACCTACCTATTGCTGT	TGTGAGACGACAAAGGGAGC

 

Amplicon 5'

Amplicon 3'

AATCCGACGGTCTGCATACCTTATAACTAATAATATTTACAATCGATAAAGGATTGGTCTACGGGATAAGAAAGTGTTTTTTTTTCGGGGGCAACGAAAGTATAAATTAAGTATAAATTGCGATAAAAAACAGAGCCGCACACGTAACGAAATCAAATCCAATGCAAGTCGAACATATAACATATAATACTTCGTCGTGGAGTTTTCGAGTTCAATGGTCATTTTTGGGATTCGGAGGCATTATTCGTTACTTACCTTTTGCTATAGAGCGCCAGGCTGCGGGTGCGACTGCTCCCCGAACTGGCCAAAATATTGTTAGTTGTATTGTTGTTGTTGTTATAGTTATTCAGGGAGTTGCTTAGATTACTGTTGTTGTTGTTGTTGTTGTGGTTATTATTGCTACTGCCGCTAGTCCCAATACCGCAGTCATTATTGTTGTTCAGATTGTTGGCCAAAAGAGCGGCTGTCCCTGTGCCAGTTGGCAAGCAGGAGGAAGTGGGCTTCAAGGCAGCCATCGCTGTATTTCCACCCACTCCATTGTTATTGTTCCGGAACGGTTGGGATCGAGCTCTCGTATGCCTCACGTTCGATTCCTGTTCGCTGCTGATACAACAAATATACAATCGTAATTTAGCAGAAGAAACTTTGTAACAAAAATAAATACATTCAGATGCACTTGAATTTCTAGACATATTAAGCAATTGAAGTGCCATCTACGCGT

CAGTCGACCTACCTATTGCTGTTCACATTAACGGATCGCGAGTGATGCCTTCGCAGCGCCTTGATCTGATTCAGCTTGTCGCCCTGCGTCTGGGCCTGCTTCTTTTCGAGCGCCGTTTGTGTCATCCTGACGGTGGACGCAATCGTGTGTCGGGCTGCCTCGAAGACATTTGCACTTGGCTTGCGCAACTTCTTCTCCGTGCAGCTGGCCAACTCAGGAGTGGGATAAACTCCAGATGCCGTACTCAATCCACTGCGATTGGCATTGGGATTAACCAGGCGCCTCAGGGATGTGGCTATTACTGTCGGTTTGTTCGCGCCTGATGGAGATTTCCTACGCCGTGTGGATATTGTATAGCTGGAGGTAGGCGGCGGATCCGCTGGTGCACTGATCGTGTCCGTATCGTTGTCATCGCTATTATTGCAATTGGGATCCTCAACGGATTGATAGAGCGCTCTCTCGTCCCGCTCCAATCGCAGCTGCTCCGAGAGTTCCTCATCCGTTTGATCCGTACAAGCTGCATCTAAGTTATCCTGGTCATTCAGGTGCATCGTATCTACCCGCTGAGAAATGCCCTTCACTCGGGTTAAAAGCAGATCGACGTGCTCTGTGGAATCGGCGGCAGCCGAGGCCAGTTTTTTCTTCAGCTCCAGGCTGTTGATACCGTACGTATGCTGGAGGATGGGAAGCGAAGTGCGCCGGCGCATTCGTTCGCCCTCCGACCACAGCCAGCTTTGTTTGCTAAAAATTTGGAAAACATTTGTGATTCTTTGAGTAAAATCGTGGGGTACTAAGAAATACGTACTCTTCCATCCACTGCTGCTGATGTCCAATCACCGATCCCGGCCGGCAAAGCCTTAGCCAGGCTATGGCCTCCAGAGCAGTGAATCCGTAATGCTTCATGATATATGCCCCTATCAGGCTGCCCGTGCGTCCCAAGCCCGCTTTGCAGTGAACTGCAATGGCTCCCTTTGTCGTCTCACA

 

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