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CRIMIC CR00873

Gene CG9304

CG9304

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:21973980..21973980	58A4	FBti0199662	79334

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAATTGCCATTGGCATGCGTGTGAGG	CAGACCTGTCATCACACAATAGGCCA

Images

Construct Design Sequences

sgRNA sequence	GGAGCCAGCTCCATACCAAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGACGGGCGACTTCTTTCAG	AGCTCCATACCAAAAGGCCA	GAGCTGGCTCCATGCAGATA	TCCCGTTCTGCTGATTTGCT

 

Amplicon 5'

Amplicon 3'

AGACGGGCGACTTCTTTCAGTTCCTGGCGAAATTCGGCTTCAACAAGGCAGATCTGACACGGCGCAATGCCTATGGATATATCTACGGCAATGTCACGTCGACGGATAAGTATGCGGTTCCTTTGACACTCGTCGTCTTGGACAGAAGGACTTTCCTCGAGTTCTACGGCAACCGCAGTCAAAGGAGCCGGGAAGCCGCCTGCACAGGGATGTTCTCCCGCCTCCAAAGGATCGCCTACGATTCCACGTGCAATCCTCGTGCCAAAAGGGACTTCCTGCGTCATGTTCCCTGTCCCGTCAATCAGCTCTGCAGCGATGAAGATGAACCCGGAAACGTAGTCCCGGGATCGCAGTTCACCTACGTTATCAACCTGGAGGCGGAACCAAGGTGAGTTATGGGTTTTTCTGGGCTTACAATTCGGAAGATAAGAATTGGATTGCAAAAAAAAACCCAATTCATCTTCAGAGACTGCCATTCTCTCTATATATTGGCCTTTTGGTATGGAGCT

GAGCTGGCTCCATGCAGATAAAATGAAACCTTATATCTTTGTTTGTTTGTTGCGGCATTCCTCTTTTGTAACGGAAAACTGAGATAACATTTGATTTGGAAATGACTTGGAATAATTTTATTTTTCCATAGAAGCATTGTTGTTTTAACTGGTTAATGTATTTTGTTGTCCCCCGCGTCGTTGACAGATTTTGGTACCTCTCCCTGGTGGCCTGTTATCAAAACAAAAGCACCTGCCAGTGGCACGCGCACGAGAGCCTGCCAAGGCTAAGTAAATTGGACAGCAATCTCCTCAACACGCTGCACTACGACATACATCTGGTCAACTTGCATCCGAACAACTCGGCCGCACATCCACTTACCTACCAGTACTCGTACGATCAGCAGAATCTACTGGAGATGTACCTGGTGTTCCTGCTCGTCTACATTGTTCTGCTGCCCATGCAAATTTATGCGGTGCGGCGGCAGAAGCATCCAGTGACCAAGTTGTTTACCGTGAGTCTTCTCAGCGAGTTCGTCAGCCTGGCTCTGATCACCGCTCACCTGATTCACTATGCGGCCAATGGAGTGGGCGAGCCAAGATTGCAGGCAGCTGGAGATGTCCTGGACATCTTGAGCCGCACCACATTTATGTTAATTCTGCTGCTGCTGGCCAAAGGATGGGCAGTTACCAGACAGCAAATCAGCAGAACGGGA

 

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