CR00873

CRIMIC CR00873

Gene CG9304

CG9304

Information

Donor Location Cytology FBti Stock number
pM37-p22R:21973980..2197398058A4 FBti019966279334

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK AAATTGCCATTGGCATGCGTGTGAGG CAGACCTGTCATCACACAATAGGCCA

Images

CR00873-7.jpgCR00873-8.jpg

Construct Design Sequences

sgRNA sequence GGAGCCAGCTCCATACCAAAAGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGACGGGCGACTTCTTTCAG AGCTCCATACCAAAAGGCCA GAGCTGGCTCCATGCAGATA TCCCGTTCTGCTGATTTGCT
 
Amplicon 5' Amplicon 3'
AGACGGGCGACTTCTTTCAGTTCCTGGCGAAATTCGGCTTCAACAAGGCAGATCTGACACGGCGCAATGCCTATGGATATATCTACGGCAATGTCACGTCGACGGATAAGTATGCGGTTCCTTTGACACTCGTCGTCTTGGACAGAAGGACTTTCCTCGAGTTCTACGGCAACCGCAGTCAAAGGAGCCGGGAAGCCGCCTGCACAGGGATGTTCTCCCGCCTCCAAAGGATCGCCTACGATTCCACGTGCAATCCTCGTGCCAAAAGGGACTTCCTGCGTCATGTTCCCTGTCCCGTCAATCAGCTCTGCAGCGATGAAGATGAACCCGGAAACGTAGTCCCGGGATCGCAGTTCACCTACGTTATCAACCTGGAGGCGGAACCAAGGTGAGTTATGGGTTTTTCTGGGCTTACAATTCGGAAGATAAGAATTGGATTGCAAAAAAAAACCCAATTCATCTTCAGAGACTGCCATTCTCTCTATATATTGGCCTTTTGGTATGGAGCT GAGCTGGCTCCATGCAGATAAAATGAAACCTTATATCTTTGTTTGTTTGTTGCGGCATTCCTCTTTTGTAACGGAAAACTGAGATAACATTTGATTTGGAAATGACTTGGAATAATTTTATTTTTCCATAGAAGCATTGTTGTTTTAACTGGTTAATGTATTTTGTTGTCCCCCGCGTCGTTGACAGATTTTGGTACCTCTCCCTGGTGGCCTGTTATCAAAACAAAAGCACCTGCCAGTGGCACGCGCACGAGAGCCTGCCAAGGCTAAGTAAATTGGACAGCAATCTCCTCAACACGCTGCACTACGACATACATCTGGTCAACTTGCATCCGAACAACTCGGCCGCACATCCACTTACCTACCAGTACTCGTACGATCAGCAGAATCTACTGGAGATGTACCTGGTGTTCCTGCTCGTCTACATTGTTCTGCTGCCCATGCAAATTTATGCGGTGCGGCGGCAGAAGCATCCAGTGACCAAGTTGTTTACCGTGAGTCTTCTCAGCGAGTTCGTCAGCCTGGCTCTGATCACCGCTCACCTGATTCACTATGCGGCCAATGGAGTGGGCGAGCCAAGATTGCAGGCAGCTGGAGATGTCCTGGACATCTTGAGCCGCACCACATTTATGTTAATTCTGCTGCTGCTGGCCAAAGGATGGGCAGTTACCAGACAGCAAATCAGCAGAACGGGA
 
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