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CRIMIC CR00874

Gene bgm

CG4501

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:14017639..14017662	34F1	FBti0199663	79335

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGATTCACGCCATTCGCGACAAGCT	GTGCGGCAGAATGGTGAACTTCTGAA

Images

Construct Design Sequences

sgRNA sequence	GGGAGAAGTGTAGAACTAATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTTCGACTTTGTGTGCGC	TGAAGTTCTCTCCATTCACCCG	GGGAGTTTCGTTTAAGTGCAGA	ATTTCACCCACACGGAGGAC

 

Amplicon 5'

Amplicon 3'

AACTTCGACTTTGTGTGCGCATAAATTATGTAAAAAGGATGCACAGTTAAAGTGGCTGCAACGACAGAATTCCACTCGCAAACTCCCATTCCCTGCATTTTACATGGTTCACCCAGCTTTTCGGCCAGCTTCCTGGCCAAGGAAGCCATTGAGGGCCATTAATCATTATATTGAAAAAAGCGCCTTTTAAAAGGAAAAGAAAAATACTAGAAAACAAATTGGTTTAGCTGGTTTAGTTTAGTGTGCTCGTAAAAATTGTTACTTCACTTGGCAACATTTTTTTTTTTTCTGTGTACATGTGTGCCGAGTTTCGGTGTCCGCTAAATTATGCAAGCCAAGCGGGCCGACAGCGCTTCTTAAAGCGAAAATCACTGACAACAACATTACTCATACGTCACGTGGAACAGCTCCCGCTGCTGTTGCTGCTGCTCCTGCTGCTGGTGGTATGTACCAGAAAATAGAAAATGCACGAGGAACAGGACCACGACTAGGAGCAGAGCCAGGACACGCCATGCGGGCACGTCCTCTTAAGTAAAAGCGGGTGAATGGAGAGAACTTCA

GGGAGTTTCGTTTAAGTGCAGATACTTTTGCAGTGACTGGAGCATTGGGAAACCCAGCAGGCGGTCCAAAATCAAGCGAAAATTAAAAGTAATTGAGGGAAATTTAAAACGAAAGCTTTCGCAATTTTACAAACTATTTATTAATAACTAATAGAATTTATTGCTGACAGAAAATAGTTAGACGAATTCGAACGACTAATTGGCATCATGCTATTGCAGGTGCATTTTGTAATCCTTTGTTTATTGATGTGCGACTAATTCGCAAACACCACACATTCCATTACACATACCCACAATAGCTAACCCCAAACCTTGAACAAAAACTCATCAGGCAACAAAGCTAATTAAACTTTTTGAACACAACTTCTCGCAGCAAGAGCTCCGGGGGATTCCGGTACAAGATTGCCAAGTCGCTCATCATGTCCAAGGTGAAGCAGGCCCTGGGCTTCGATCGCGTCCTTACACTGGCCAGTGCGGCAGCTCCCATGTCGCCGGAGACGAAGAAGTACTTCCTCAGTCTGGACCTAAAGATTGTCGATGCCTTCGGCATGTCAGAAACGGCCGGTTGTCACACCATCTGCCTTCCCGATTCCGTGGGTCTGAACACAATCGGCAAAACTTTGCCCGGCTGCGAGTCCAAGTTCATCAACAAGGATGCCAACGGTCACGGAGAGCTGTGCATCCGAGGACGTCACGTTTTCATGGGCTACATCGACAACAAGGAGAAGACCGAGGAGTCGCTGGATGACGACTGCTGGCTGCATTCCGGTGATTTGGGATTTGTGGATGACAAGGGTTATGTTTCACTGACGGGACGATCCAAGGAGATCATCATTACCGCCGGCGGCGAGAACATACCGCCAGTGCACATCGAGAACACGATCAAGAAGGAGCTGGATGCCATTTCCAATGCCTTTTTGGTGGGCGAGCAGCGCAAATATCTCACTGTTCTGATCACCCTAAAGGTGAGTCTTCAATCGAATCCAATTGGTTGGAAAGTATTTAATTGTTTTTTGATTTCCAGACCGAAGTGGACAAGGATTCCGGTGAGCCGCTGGACGAGCTTAGCCACGAGTCCTCCGTGTGGGTGAAAT

 

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