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CRIMIC CR00875

Gene tun

CG8253

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:15851138..15851177	52C8	FBti0199664	79336

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGGAGAATACGCCTGAGCCTAATAA	GATGCTCAAGAGCGAGATTAAGTCCC

Images

Construct Design Sequences

sgRNA sequence	TCGGTCCCCCGATGAATTGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGCGAACTTAGTGCCCGAT	GCCTGCCAAGTTGCCATTAC	ATCCTGGCGCGTGATATGAG	TAACAAGGACTCGAGGGGGT

 

Amplicon 5'

Amplicon 3'

AAGCGAACTTAGTGCCCGATGCAAATGATACCCGCTGCCTTGCCCACTTGTGTGAATGATTATCTCATGCCTGGCCAGTCCCGGCGGACGAGCGTTTCTTCTTTTAATTATGTATATTTTATTTTTGTGCGTTATCTCTTTTGGCAACAACATTCGCCGCTCGGCGCTGCCATGTCCTTCGTCCTGTGCCGCCTCCTTTTCGGATGGTTTTCATTATCCCATCGCTTATTTGGTGGCTCCACCTCCTCCTCGTTCGTCGGCGTGCCCCGCTTTGCATTCAAATTCGCTCATTACCCACTCACTCCTGTGTGTCCTGCGACTCCGCATCCTTGCGCCGCCTCCCTTCCACCACTGGCTTCCCGAATTCGAATTCTGCTTCTGAGAATCCTGATGCGGCGCCGTTTGGCGCTGCGCAGCTCTAGAAATTAGGTCAAACGGTCATGTCTGCCAACAAGAAAAGCGTGCCACTGTGTTGGTGGTCGCTGTATCTGTGTGAGTGTGCGGTAGGACGGGCGCAGGATGGGGGTTCGTTTTTATGGCCACATGCGAGAGGCGCGTTTCGACTATAAAACAAACGACCGACCAACAACAAAAACATTCGAAAAACCAAAGCAAAAATTGAACAATACATACTACATACTATAATACTTTATAATATACATACATATTGTATGTACTTATGTATGTGCTATGTGGCATTTTCCTGTGTTGATGCAATATTTAAAATGCTAATTGCAAAAATGGCGGCAATGGCGGCGACGAGAACTAAGTAATGGCAACTTGGCAGGC

ATCCTGGCGCGTGATATGAGTTTTATGTTCTGCCACCCACACACCCGCGCACACACGAATGTAAAAAAAAAAGCGGCAAAAAATAACAAGTAAAAGGCGCAGAACATAAAAAAGTTTTTACACACGCATACCCATTTACATGGCCAGATAAAATGTCGTTGCGTTCTCTGACCTCATACCACCCAACATTCAGCCTTCTAACCGCCCATCCGGCCAGCCCCCTTCCCCCTTCTCCCGCAGGACATGTTCATTGATTAATTCGCAACTGCTCGCGCACTCACATTTGCACTCAGATTCATCTCTCCTTCCAGGAAAACTCTGGAAAACCCACCGCTCTCGTTTGCCTCGCTTTCGTCGGCATATACACCACAGCAGCGCCATCTATGGGTCGCGTGTTGCAGCGGCTCGCAAGTTTTAGACACTGTCTTTTAGCTTTTGCGCCATTGCGCACTCATAAATAGATAGTTTTGAAACATATTTCAAATAAATATAATTGTTCATATTATTAGGAATTTGTTGGCTTTTGGGGTCGTTAAAATTCCCAGCGAAATATCCTTTTCTTGCCTTCTTATTTAAAGGATTCACAGGTAAATGGCAATGGCGCGATTTCTTTGTGTTTGCGTTTGAGCAAGTCACTCAAATTGGGCCTTGGCTTAAACCAGCCCACCACCAACAGAATAACGCCAGCTCACGATATTGCCGGGAGCCACTTTCAGTCTCGACTTTTGACCGCGCCACTTGAGTGGGCTGTGCCACCTGCCTCTTGCCTCTTTCCTCTTGCCTCCTTCCATGCGAACAATTTAGAGCACCCAATCCACCACCCCCTCGAGTCCTTGTTA

 

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