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CRIMIC CR00877

Gene Tehao

CG7121

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:13437859..13437859	34C1	FBti0199665	79337

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGGCAACGACGATCTCATGGAGCTT	GATGATTCGCTTCCGTTTGTCCTGCA

Images

Construct Design Sequences

sgRNA sequence	GAAGTACCGAGTTCCAAGTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCGCCTTATCGCTGTGGTT	GTTCCAAGTGGGGCTATCGT	TGGAACTCGGTACTTCCTGAT	AGAACACGGTGAGCAGACAG

 

Amplicon 5'

Amplicon 3'

TTCGCCTTATCGCTGTGGTTGCGGCTGCTGCTATTTCCCTTATCAATACCCGGGGCACGAGTAATGGCTCATCGAGAGTTCCATGTCTCCCCCTGACAGGCAAGTGGGGTTATCGGGTCCTATCGTTCAGATACTAATTGCAGTGTTATTATTGTACACAGATTGGCGCCAAAAAGTATGCCAAACATTATTAATTGATTGATCCTTATCTGGCTGTAGTAATTCGTGCTCAAATCAGACAAAATATTTCGTATTTGTTACGCCACAAGTACTACTATGTATATCTAAACAATTCATTATGAAAAGTCATATGAAACGTAAGACGTATTTTATTTTTTTTGTATAGTTCTTGTCTATGGCAATCTTATTGTCGTTTGATAAGATGTGGTCGAATAGAAATTAATTAGATATTTTTGTGGCTGATAGATTTTAATTATAGAAAAAAAGGTTAAGCTTTTTACCGATCTTAAAAAATGGCATTGAACAATGTTCTTAATACTATTGAGATTTACAATCAATCTGCGCGAAACAAAACTTTTTATACTGATTTTCTAGAAACCTAAGCATTATCATGTTGATTTGCAGAATTTATTTGTAGTCCCCATTTTGAATTAGCAATACCATAGAAGTTTTTTTTTATTAATTTAATAAAAGTAGCAAATTGGTTTTTAGATGTCGCAGACATAAGCATTATGCTTAACATAATGCCTACTAATCCCCTCATAAATGTTTTTAAAGGCTTCACCACTTATAAAATACTGTTTATAAATCATTTTTATATTGTTAAATAAATATGTAAGTAACATGTAAAAATTATAAATAAGCTAGTTAAAATAGCTTTACCATTTTCGATAGCTTATTTAAAAGAATCAATGTTATTGTATAGCATTAGGTCACTAGTTTTGGGATGTAGATAACGATAGCCCCACTTGGAAC

TGGAACTCGGTACTTCCTGATTAATGTCAATCTAATCCCTCTGAGATCTCCTCCTCCTGCAGCACAATTTTCGCACCATTGACTTCGCTGAACGTTTTGCGTCTGAACAAGAACTCGCTGACCGCGATGTCGCCGAGTGTGTTCCAAGATGTGGTCAGTCTGAACTACATCGAGATGGTGAACACCCAGTTCTATGGGGCCACGCTCCTGATGAACTACGAGGCTGTGGTGTGCACCAATGACGAGGCGTGCCAGTACAAGTCGGCGGAGTGGCAGTGCGATCCCCGATGCATCTGCTGGGTGCAGCGGAGTGTAGGTAGTCTGATCGTGGATTGCCGCGGAACGAGTCTCGAGGAACTTCCGGATTTGCCCCGCACCACGTTACTGAGCACGGTGCTCAAGGTGGGCAACAATAGCCTCACCAGTCTACCCACTGTGAGCGAACACAGTGGTTATGCCAATGTGAGTGGACTCTTTCTGTCGGACAATAACCTGACCAGCCTTGGAAGCGGTGACCAGCTGCCCGATAATCTCACCCACCTGGATGTGCGAGGCAATCAGATTCAGTCACTCAGCGATGAGTTCCTATTGTTTCTTCAGGAGCCGAACAACACTATGACGCTATCGTTGTCGGGAAACCCCATCACCTGTGGCTGCGAATCCTTGTCGCTTCTCTTCTTTGTGCGGACCAATCCGCAGCGGGTACGGGACATCGCCGATATTGTGTGCACCAAGCAGAAAAAGTCCTTCCAGCAGATGGAGGCCTTTGAGCTGTGTCCATCGTATGTCCTACTCATCAGCTGTGTGGTCGGTGGCCTGGTGATCGTCATCTGTCTGCTCACCGTGTTCT

 

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