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CRIMIC CR00880

Gene CG8613

CG8613

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:14352743..14352763	50F6	FBti0199668	79340

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTTAGCCTGGCCAACAGCTAATGG	TTTATCTTCGTGGGTAGGCAGGGAGT

Images

Construct Design Sequences

sgRNA sequence	ACTGAGAATTCGAAGTGCGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCGGCCGCATAGGTGTAAG	TTGCTACTCACCAGTCCACT	GCGTGGGTGTTATTCCGTC	ATGAGGGCATTCCATAGCGG

 

Amplicon 5'

Amplicon 3'

ATCGGCCGCATAGGTGTAAGCATTTTCTACATAATTATCAAAGCAAAGATATACCGTATGCATTAATAAAAGTATGAAAAATATTAAGCTGCACATTTTGAATTGAGCTGATTATTGGGGGAACAGCCTGAAAGTAGGCTACAAAACTCTTGTCTTCAGTACTCTTTATCGTGATTCCCACGACGACTTTCTTGCTTTTACATCATAAACTCAATGTGGTAATAAATTTAAAAATAGTTATACTTTTCTGTCATATTCACCAAAAGCTGGAAATTTGTATTAATTTTAATGTTGATATGAGGTCAAACGCATAAAATAAATGTATAAAAGATGTTTTGCTTACTCCGAAATCAAGTCTCAAAAATAAACCCTTGAAATATTAGTTTGAAATCGGAATTTTTTTAAGTAAAAAATAAGTTCACAACAGTTAGTTTAAATTCAAAACAATCGATAAGTGCTCTGAAAAATCGGTTTCGCTGGAAATTGACCATCCGATGGTATCGAATAACATCGCATTCGGTGCCAGCCCTATGCCGATAAGCTAAACAATATTAAAAACATTGGTCAATTCGCGTCGATTGCACACGTTGCACTTTTGTTGGCATTGGACGAGGTCGAGTGAAAGCACAGCGCAAGCCCCGAGAATCCCGATTCGTTTGCTATGTTCAGGGTCAGGCCGCTCCTCCCCAGGATACGAAGCTCCCTGCAAACGACCTTGAAACTCCAAGCAGATACATCGCAATCCGAATCCGAATCGTTCCGTCGGAACCTACGACTCCTACACAACAGTTGTCGTAGTCGCCCTGTGAGCAAGTAAGTCTTGTGCGTCCTATCAACCACCTGTTTTGATATCATTTTTGTGCGCGCAACGCAGCCAGAAATTCCGCACAATGGCCACCGGAGGAGAAGCATCCAAGGAAGTGTCCAAAGAGGAACCCGCCAAGCAGGGCAATCGCTTGGTTGCCTCGAAGTCGCCATATCTCTTGCAGCATGCCTACAATCCAGTGGACTGGTGAGTAGCAA

GCGTGGGTGTTATTCCGTCTTAAATCACGTGTGATAAGAGATAAGGTTGATAAACAGCACAAACACTTGGGGAATGGTAATGAAAACAATAATATAGGCTTGTTTATAACTGGTTGGGTTACTTTTGATTTACATTTTAAATTACTTACTGATAAGTCGCGTCTCTTGGTTATTATATAAACTTGTTATGTGGTCGTAATCTAAATATATATCGACTATTTTAAATTGCCATTTGACCCGTTGTTACAGGTACCCGTGGGGCGAGGAGGCTTTCGAGAAGGCCCGCAGCGAGAACAAAATCATCTTCTTGTCAGTGGGCTACTCCACCTGTCACTGGTGCCATGTAATGGAGCACGAATCGTTTGAGAACCCGGAAACGGCTGCGATTATGAACGAAAACTTTGTAAACATCAAGGTGGATCGGGAAGAGCGACCGGACATCGATAAGATATACATGCAGTTCCTACTGATGTCCAAGGGCAGTGGTGGTTGGCCGATGAGTGTGTGGTTAACACCCACTTTGGCTCCACTAGTGGCGGGAACTTACTTCCCACCCAAATCCCGCTATGGAATGCCCTCAT

 

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