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CRIMIC CR00886

Gene qkr58E-1

CG3613

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:22414289..22414307	58D8	FBti0199671	79343

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGGTGATGCCGATTTACCTATCGATG	TTTCGGATATGGAGCCGAGCGATATC

Images

Construct Design Sequences

sgRNA sequence	AAAAAGTAAGGGATTAAAGGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGATGATGAACGACCGTTGC	TCAACAGATCACGGGACCAC	GGAGGGTTTTCTAACAATGGTTG	AAGACCAACGCGTATCTGCA

 

Amplicon 5'

Amplicon 3'

CGATGATGAACGACCGTTGCTTGGTTTTGCTATTTTGAAAAGAATTTTATTTGTTGTTTAGACTTTAAATTGTTTCTTGTTCTGTCTCTGTGGTGCTGTTTTTTGGTTTTTGGTTTTTCTTTTGCTTAGCATATATACATTGTAAAAAAAAGAAAAATATATTGTTTAACCGGTTTTGAATGCACTACACCCTACGAAACGTTATTGTTTTGAGTAAAATGCATTTCGTTTCATCACGTTTCAAAAAAAGTTGTTCCACAAACCTTTCAGCTTGGATTTTATAGTTTAAGCATGTTCAAATATTTTTGGGTTTCTTGCCATGAAGTTTTATGGTTTTGTTTTCAAAGTGTGAATTTCAGTAATTTGATTGTTTGATCGATTGTTTTAATATTTTGGCTCTGGCTTCTTGTGAGTATGTTAGTAGTCGTCCAATTAATGAAGATGATTTACTGGTTGCTGTTCGGTTTAGTTGATTTGGCCTCTGTTCAGGCATAGTGCATTTAAAATCTTGTTAAAATGGGATGGGTTGCCGTTTTAATAATTTTGGTACGCGGCCTTGTTTCTTTTTCTTTTTTGGTTTCTGTTTTTTTCTGCGTTTTCAATTTATCGGCGCTTAGTTGGATAGATTTTGTTTTGTTTTCGAGTTTTTTTTTTGTTTTGATTTGCGCGAGTTAAGTTTAAACGTTTACATTGCTATTTGTCGTATTCTTTTTCTTTTCTTCATTTTGTTTATTTTTACATGCCTGCTGAGGGGAAGAGATTATTGGTCTAGAAATCTAGGTATCTTACCTGGATAGGCCGCCGCGTAGGAAGAGGAGGACGAGTGGGGGTGATACTCACGCCGATAGTCGCGCTCGTAGGAGCTACTGTCATACTGGGCGTGGTGGGTCGAGTGCTTTGATGTTTGGGGCGGCGGTGTGGCATAGCGATCATATTCTCCATGGTGGTCATATAGCTCGTGGTCCCGTGATCTGTTGA

GGAGGGTTTTCTAACAATGGTTGATAACACAGATGCACGCTATTGAAAGCATAAGAATTTAACTTCTAAAGGCATCATTAACGCGATTGAACTTAGCTAACCTGTGCGTCTTTACGATCCCATAGTTGGGATCGTCCATCACATATCGAGCCTTCTCCAGAATCGAATAGACTTTGGGCTTTGAAGAAGCAGGGGGCGGGGTGCTGTGACGAAAGGAATTCCTTAGTAACCAATTAAAATTCAACGAAAATTGACAAGCCTACCGGGAACTGTAGGCGCCATGATCGCCATAGGATTTGGAATAGTGGTGAGACTTCTTGTACATGCGCTCCTTGCCATCCATCTCGCGTAGTTGCTCCAACCGAATGTCGTCGTTGGCGTCGGGTATCATAAACTTGCGGATCTCGCCTAGCGCATAGCTGATCCTGTGATAGGCCTCCGCTGGAGGGGCCACCGTGGATATCTCCACGTGCAAATCGCGGCTGAGATGGGCGTACTTTGGATTTCCGGAGCTGCGAAGCTCCTCCTCCTTACCGTGGTCGCGCATCGAGTTGCGCCCCATAACCACCATTTTGCACATAGTCTCTTCCTGCAGCTGCCGCAGTGTGTTGCCCTTGGGCCCCAGGATCTTGCCAACGAAATTGAACTTGGGGTACTCCTTGATGGGGAACAAGACTTTCTGGGCCACGCGGATGGGCTTTTCGCTGTACACGTTGGCATATATCTCGGGCTTTGGTATGCGTCCGCTGACCAAGATCTTCTCCACCTCTGCGGGGCCATGAAAAATTGCAATTAGATCCACTATAGCTTACATGTCGCAGCGCCTAACTCACCGTCGTCAAGTAAGCGCTTGGTGATGATGTGCTTCTTCTCGAGCGTCTTCTTTTCCAGCAGGCACTCCTGCAGATACGCGTTGGTCTT

 

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