CR00887

CRIMIC CR00887

Gene CG5168

CG5168

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:10424134..10424146	31E1	FBti0199672	79344

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACATAACTGTGCGTGGTCCGTAGGTT	TATGAGAACCTAAGCAGCAGGGACTG

Images

Construct Design Sequences

sgRNA sequence	GGGGGAAGGGGGTGCGACAGTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTAGGCGTACCATCGGACTG	CCCTTCCCCCTTTCACCATC	GGATATGGGATAAGCGGGTCA	GTGGAACAGTTGTCGCACAC

Amplicon 5'	Amplicon 3'
GTAGGCGTACCATCGGACTGGATCCACCTTAAATAATATGGCTGCCGAGATAAAACCAGCTCAGCGGACCAACAATGATCGCTTTAACACCTCCAAAAAGCCGGAATTGCTGAGTAAGCTGGAGGGCAGCAGCGACGATGTGAACGCAGCGATCCTAATTCCGGGCGAAAACGGCGTAATTAGCGTTTCCGATGACAAGTGCGTAGCTGTAGCCCATTAAAAATATACACAAATGGTATGCCTGACATTTTCATGTTTTCCCCAATAGAACCGTTCGCGTTTGGCTAAAACGCGATTCTGGTCAATATTGGCCTAGCATTTGCCAGTATATGCCTTCCGGCTGCACGGCCATCGAGTACGTTTCGGAATCCCGGCACCTGTACGTCGGCCAGGAAAACGGAACGGTCACCCAGTACGCGCTATCCGAAGACTGCAATCGCCTGTCCTTCCTGCGGGATTACTTGTCCCATCAAGCGCGGGTCATGGCCGTGGTCTTCTCGAAAACCCACAAGTGGATACTCTCCGCCGGCAAAGATAAGCAGTTCGCCTATCATTGCACGGAGAGCGGCAAACGGGTGGGCGGATACAACTTTGAGACACCATGCACGGCTCTCCAGTAAGTATGATAAATGGATGGTGAAAGGGGGAAGGG	GGATATGGGATAAGCGGGTCATTTAATTGGATTAAACTGCTTTCTTTGACACGGCAATAAACTGCAATTCTTCACGGCTATGGAATTCATTTAGAATTTGAGAACCTTCTATTGTTAATGACGTGTCAACTGAGTCATTTTATCTACACAAAATTTTGGCATAAATGTGTGCACTGTGCGAAATATTCTATAAGCAACATATAATTAATTATAATCTGCGTTTAGGTTCGACGCTTTGGCCAAGTATGCCTTTGTGGGCGATCATGCTGGACAGATAACAATGCTAAGGTGCGATGTTCAGGGAGTGCAACTAATTACCACTTTCAATGGACATTCGGGTAAACAAGTTACAAATGATATTTAGTAAGCTAATTATATACGAATTGGTTAATTTTTAGCGGAAATCCGGTGCCTCAAATGGGTGGAAGGCCCTCAGCTCTTGTTTAGCGGAGCTTGCGATCAGTCCGTGCTAGTTTGGGATGTTGGTGGCAAACGTGGCACCATTTACGAGCTGCAGGGACATAGGTATCTCCAATCTTAAATAATATTCATAAAATAAGATAGTAATCGGCACTTCTGATTATGTAGCAACAAAGTTTCCGCCCTGTCGTACGCGAATCACACGCAACAACTGATAAGCTGTGGCGAAGATTCAGTAGTGGTTTTTTGGGAAATGAATGCAATGCGGAAAGAGGTTCCTGGCTGGGTGGATACCAACAACTGTCAGCTTTGCTCCAGGCCCTTCTTCTGGAACTTCCGCTCAATGATGGATCAAAAGCAGCTGGGTGAGATAAATAGCTTACACTTGAGTTACTCTTTGTTTTACAAGTAATTTCTGACTGTCAGGAATACGACAGCATCATTGTCGCCACTGCGGCAAAGCCGTGTGCGACAACTGTTCCAC

Amplicon 5'

Amplicon 3'

GTAGGCGTACCATCGGACTGGATCCACCTTAAATAATATGGCTGCCGAGATAAAACCAGCTCAGCGGACCAACAATGATCGCTTTAACACCTCCAAAAAGCCGGAATTGCTGAGTAAGCTGGAGGGCAGCAGCGACGATGTGAACGCAGCGATCCTAATTCCGGGCGAAAACGGCGTAATTAGCGTTTCCGATGACAAGTGCGTAGCTGTAGCCCATTAAAAATATACACAAATGGTATGCCTGACATTTTCATGTTTTCCCCAATAGAACCGTTCGCGTTTGGCTAAAACGCGATTCTGGTCAATATTGGCCTAGCATTTGCCAGTATATGCCTTCCGGCTGCACGGCCATCGAGTACGTTTCGGAATCCCGGCACCTGTACGTCGGCCAGGAAAACGGAACGGTCACCCAGTACGCGCTATCCGAAGACTGCAATCGCCTGTCCTTCCTGCGGGATTACTTGTCCCATCAAGCGCGGGTCATGGCCGTGGTCTTCTCGAAAACCCACAAGTGGATACTCTCCGCCGGCAAAGATAAGCAGTTCGCCTATCATTGCACGGAGAGCGGCAAACGGGTGGGCGGATACAACTTTGAGACACCATGCACGGCTCTCCAGTAAGTATGATAAATGGATGGTGAAAGGGGGAAGGG

GGATATGGGATAAGCGGGTCATTTAATTGGATTAAACTGCTTTCTTTGACACGGCAATAAACTGCAATTCTTCACGGCTATGGAATTCATTTAGAATTTGAGAACCTTCTATTGTTAATGACGTGTCAACTGAGTCATTTTATCTACACAAAATTTTGGCATAAATGTGTGCACTGTGCGAAATATTCTATAAGCAACATATAATTAATTATAATCTGCGTTTAGGTTCGACGCTTTGGCCAAGTATGCCTTTGTGGGCGATCATGCTGGACAGATAACAATGCTAAGGTGCGATGTTCAGGGAGTGCAACTAATTACCACTTTCAATGGACATTCGGGTAAACAAGTTACAAATGATATTTAGTAAGCTAATTATATACGAATTGGTTAATTTTTAGCGGAAATCCGGTGCCTCAAATGGGTGGAAGGCCCTCAGCTCTTGTTTAGCGGAGCTTGCGATCAGTCCGTGCTAGTTTGGGATGTTGGTGGCAAACGTGGCACCATTTACGAGCTGCAGGGACATAGGTATCTCCAATCTTAAATAATATTCATAAAATAAGATAGTAATCGGCACTTCTGATTATGTAGCAACAAAGTTTCCGCCCTGTCGTACGCGAATCACACGCAACAACTGATAAGCTGTGGCGAAGATTCAGTAGTGGTTTTTTGGGAAATGAATGCAATGCGGAAAGAGGTTCCTGGCTGGGTGGATACCAACAACTGTCAGCTTTGCTCCAGGCCCTTCTTCTGGAACTTCCGCTCAATGATGGATCAAAAGCAGCTGGGTGAGATAAATAGCTTACACTTGAGTTACTCTTTGTTTTACAAGTAATTTCTGACTGTCAGGAATACGACAGCATCATTGTCGCCACTGCGGCAAAGCCGTGTGCGACAACTGTTCCAC

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