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CRIMIC CR00888

Gene CG1513

CG1513

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:9886110..9886110	46C2	FBti0199673	79345

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGATTGTCAGCCTGCAGATTGCCAA	GAGCTCAATCCAAGGCACCGTAAGAA

Images

Construct Design Sequences

sgRNA sequence	GCCAGCAACTGAGATGGCAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCACTCATATACCAGCGGC	AGATGGCAAAGGAAGCGGAT	TCAGTTGCTGGCTTCATTTGC	AGGGTCAAGCGGTTCGAAAT

 

Amplicon 5'

Amplicon 3'

AGCACTCATATACCAGCGGCAGACCTGGACGATTCTGTCGCGTGGATCATCCAGATCCGATATTCTATTGGGTAAGATGCTTATGAGTTATATTACTGATATAACGCCTTGAACTTGACCATACTTTATAAACAGATCGGGATGAGCAAAGTAATCGGCGTACATTTCCAGGAGCGAGCGGCGCTCCAGAATAAACGTGGGCAGTACCACTTTCGTGAGGTCCATGCCGATTTTAACCTGGGACAGCAAGTGCGTAATCATCGATCCGTGCGCCTCCATACTGAGGTCCGTCTCTTCCTCCTCCTCGTACAGGGCGTCGTAGTCTATTCCGTTATCGTAGCTGGCACTTCGGGAACTTACTGCGGTCCTCGCTGTCTTAATGCTGGTGTCCGCATCGGCAACACTTTCATCGATCTCTGGCAGCGTTTGGGGGGCAACTGAATCGCCCACAGCCACTGGCTCGGCGTTGGCAAATCCATCCGGCGCCTTTTCATGGGTAGGCGATGTGGTTTCCGCAAAGCCGCGCGTTGCGCTGTTCAATGAAAAATGAGAGTAAATTTAGGACTTGTTGATTAGGATTCCGGCGATGCCTTGGATAAATCTTTAAGCATAATGTGCGTGTAAGTCACGGGGCACAACAGCATCCGCTTCCTTTGCCATCT

TCAGTTGCTGGCTTCATTTGCAATTTATGAAGGAAGCACTTACGTCGACCTGGCACACATGTATGTACATTGTATACTGCACATTCTCACCTCAGGCTAACACGACGGACCCACTTCTTAAACATGGTGGTTGGTTTCAGGCGCTGATGCGGGCCGTATGCGTTACTGCACTCCCAGCGTCTGCATAATTTCGGACATAAAACGGAAGCTACCGAATCGAAACGTAGACAAAAGCCGCCGAATAGTCTACGCTATGTACTGGACAATAAAAAAACCATCAAACGGAGAGCCGAGGGGGGCCACTTGCACCCAAAGGAGCTCTTTGACACTGCGAGGCGATGACGAACGAAACTAGAACTGACAGGCATACACAAAGGCGACTTTAGCTACGACTAGGCCGAAGGATGTCGATGACGAAGCGAGTCGCCGACGACAAAGCGTTGTCAGCTGTGCCAACAAAGCTTCGAAGGGAGCCCGTTGAGGCGGAGGAGAAGGTTTTGTATTTATTATTTACACGCGACTTGGCAGAAATTACAGGCTGCCCCGAAACTACGACTGCGACGAACTTCGGGCATTGCATTTTGCATGCGCTGCTTAGGCGCATGCGCCTTTCCAAAGGAGAAGGAGGAGTAGCAAAGCCGAAGCGGGGGGTTCCGGACTGTGGGCAATGAATGCGTAATATTAGCACGCAGCACACATCACACATAACATAGCACGCTTGCCGGCTGCCGCATCGCATCTGATTGATGGCCAGTCGGCGCACTTGGTCAAGGGCATGTCTTAGTTGCTACTTGTAAGACCTGCACAAGTGGTCTACTTACCAGCCAATCGGGGAGCTGCCAATGCTGGTGAAGGGATCGTCGTAGGCATCGTAGAAGTCCTCCTCGCCCTCGCTGCTCGAGTATGAGGTCGCTGGCACAATTAAACCCAAAGGAGCGGTGGTGGCTGCCAAGATTAATGCCAGATTATCGGGGGTAATGCAAATTGAGCTTTCGCATTGATTTCGAACCGCTTGACCCT

 

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