CR00891

CRIMIC CR00891

Gene Slik

CG4527

Information

Donor Location Cytology FBti Stock number
pM37-p02R:24400158..2440018060C8Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band short band AATCATCATCGTCAGCAGAAGCAGGG TTAGTTGTGGTTGTAGCTGCTGCTGC

Images

Construct Design Sequences

sgRNA sequence GTGCTGGGCACAGGGATCCATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGAGTCATCGTCCAGGTCGA CAGCACCGCACCCTTGTA GCACTCACCTCGGTCTCATC TGCCCTTTTCCTAAGCCGAG
 
Amplicon 5' Amplicon 3'
AGAGTCATCGTCCAGGTCGAGCTGGAGCGCCGAGTTGCGGGGTTCCTGAGAAAGATTAAATACAGCTTATTAGCATATTCATACGTAGTGTCGTTCCTAAATAATTCCTGTTCGGCTAGCAGATCTACTTGTCTCTTACTACAATAGCGTTAGCAATCCTACGGCCAAACTATGCTTACATTCGGCATGCCGAATAAGCATAATATTCGATTCTGAAATTCCACCGAGATTCATTTTATTGCCATACCCCAAAACAGCCCTAAAAGAATTTGTTTACATCCGACGTTGAGCAATAAACGCATTGTTCGCAAATTCTATCTGTTATTGCTCCATAAACAATATCTAAGTCTTAACGTAAGGAGCTTATAGATAGTGAAACTGACACTTTTGTCAGCTATGTGCTGGTCAGACTGATTAGTAACTTAAAACCGTGTGTGATTGAAAGTGTGCTGCCTATAATTACCGCAAGAAACCGATAGCGAGGGCTTACGGAGGAAGCCAAGTACAATAACAAACACAACGAGCGAAGTATGCCTCCGACAGAGGAGCTAAGTGATAAGCGGCATTTGGCAATGCTGATAAAATAAGTAAACTAATTGTGGGTCCGCTTTTGAAGGACAGTGCCTGGGATGCAGAAACAGAGGAGGGGAGGGGTGCGGGTGCCAGGAACGGACGGAGCACGGACGGCTTGCTGTAGAACGACTTACCAACCCTCTGATAGAGCGAGCGCCGCTTCACTTGGCGTTTAAGCTTCTATATCAATTATTTCCAAGTAACCACATACAAATGATAAGAACAAACATTGCGACATTTTGGCGGAACAAAACAGAACGTAACATAAACGCAAGGCAAAGAATAGTCGAGAAGAAACGAAGATAAACATATAAAATAATGTATCGCGCGGTGCGATTTGGTTGGGTTTTCAGGCGGCAGCGTGTAAGAGAGAAGAAAGAGCAGTTGGAGAGGTGAGAGAACGTAGAAATTATCATCACATCTAGCAGTTGGACTACAAGGGTGCGGTGCTG GCACTCACCTCGGTCTCATCATCCACCACCTCTTCCACGACCTCGGCCTTGTACTCAAGCAGCAGATCCTTGATAGGCTTGGCATCCAGATTTCTGTTGATGAAAGCGTGCTGCATCAGCACATCCGTCGTGGGCCTCACCTGCGGATCCTGTTCAGAAGGAATACAAAAGTAAAGTTAGAATGGAATGTAAATAACAGATTTTAGGGTTACCCACTTTGACTAAGGACTTCTTCAGGAAGTCGTTAAACTCCTTGCTCCATCGAGAAGGCTGCTCCAGCTTGGGCGGCTCGCTCTTTTGTATTTTCAAGAGCACTCGCATGGGCGACATCTCGCTGTTGGGCGGCTCCATCTGCGCCAGCTCAATGAGCGTGATGCCCAGTGACCAGATGTCCACTTTGTGATCGTACGGGTTGTCCCGGAAAGTTTCGCAAAGCACCAGCTCCGGGGCCATCCAGTAGGGAGTGCCAATGAAGGTGTCGTGCTTTTGCATGGTGTGCTTGTTTTTGGCCGAAACGCCAAAGTCCGCTGAAATGAGTTGATTTCACTTTGAGATCGTCTCGGCTTAGGAAAAGGGCA
 
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