CR00892

CRIMIC CR00892

Gene CG3500

CG3500

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:23356692..23356692	59D8	FBti0199674	79346

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGAGGGTTTGGGTCAACGAAATGA	GATACGAAGATTCTGGGGATTGGCAG

Images

Construct Design Sequences

sgRNA sequence	AGGCCGCAGCAAACGCCTAAAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTGGCCGACTACACAAAGA	CGCAGCAAACGCCTAAAGG	TGCTGCGGCCTAATTCAAGT	GGGATTTCGGATGGGGTGTT

Amplicon 5'	Amplicon 3'
GGTGGCCGACTACACAAAGAACTCCATCCGCCTGGGCGAGCAGTGGTGCCGCGGCGTGCCCATCGAGGTGGCGCCCATGGCGTACGTGCCCATCAAGCTGCACATCGAGGCGCTTTTCGGCGGCGAGGCCTCGTTGCGTATGGCCAAGGTGAAGGCGGGACCCATTGTCACGGACAATGGCAACTTCCTCCTGGACTGGAAGTTCATCGCCAACAGGGAGTACGACTGGGACGAGGTCAACCGCGCCATCACCCTCATCCCGGGCGTCCTGGAGACGGGCCTGTTCGTGAACATGGCCCACAAATGCTACTACGGCATGGCCAATGGCTCGGTCAAGGTCCAGAACAAGTAGTTCTTGGGCGGAGTTGGGGGAGCGCTTTTTGCATTTATGTTTCACCAAGTTGTTCCTTAAATTTTAGGTTTGCCATTTGTAATGGATTTAAATACATATTGAGTTCTTAGCCGAGAAAACGATGTGTCCTAACTTGAAAGTTTCAGCAAACGAATCTTATTCAGCTTCTTCATTCAAGTATAACATCGAAATACGTCCAAATCACCACAAAAGGGTATCCATTACACTGAAATCAGACCCACTAAATCTCAATTGGTTACAGTTCCACTACAATAAAGTGGATGTCGAATGTCAGTTCACATGCGCTTTGTATACTTTTGTCTCGATTGCGGCTACAAAAATAAATAAACATTAAACAAGCTAGATCTAGAAGGCCTTCTTGGTCCGCCCAGGGAGCAGGGCCTCTTTCAGATACTGGAACAGCGACAGCAGGCCCTGCTTCTTGTTACGCGAGAAGTAGTTGCTGACCACGTCCGGACTGCGCCGGCTCTGGTCGCTGACGGTGGTGGGCAGGACACTGTCATTCGCACTCAGCGAGACGAACAGGGCGAAGGGCACGATCCACATGCAGATGGTAAAGTAAGCCAGCACCTGAAAGTGTAAATTTCCTTTAGGCGTTTGCTGCG	TGCTGCGGCCTAATTCAAGTTGTATGTAAACAAACTGATGATGTGGATACACAATGCAATGCATATTGCATATAATGGCTGTATAAAAACGTACATACGTTTGTACGTAGCGAATGCGCTTTCCATGACTAATGATTTATGCAATACATATGCTCACCTGCGTGAAAGGCACATAGACAGTGACGAAATGCTGGAAGGCCAGGAAGTGATTGACCACCAGCATGGCCAGGGAGCCCAGCAGCGGCAGCGAGAGCAGGCGCACGAAGGGGAATCCACTCATGATGCCCAGGTGGAAGCCCTGCGCCACCAGTCCGCAGATGATAAGGCTCCACGGCAGGTCCTCGAACAGCAGCAGCAGGATGTAGACGAAGATCGTGAAGCTGATCAGAAATAGGATGCACTTCCTAGCCGCCGTCGTGTACTCCTCGGCCAGCTCCGCCAGATAATAGAGACCAGCCACTGGAAATACAAATTGCAGACGTTGCAATTGGACAAGTGCGGCTGCACGGCGACAGCTCACCAATCGACAGGGTGAGGAACACGATCTGTATGGCCAGCGAAACCCATCCCAGCAGGTACAGGACGGCCATCTTGCGTACTTCAGGCGGAATCGAAGGATATATACGGCTCAAATCGGCCGACAAAACAAAACAATCCGATTAGAGTGGCGTGTGGTGTGTGGCTCGAAATACCAAACATTTAATCAGCTTGTATAGAAACGGGCACACTAATGCGCAGAGCTGCCAGATATTCTCGTTCCAACGCTTTTTCAAATATTTTTTGTGGGAAACATGAGGAATTTAGGATTGAAATCCATATGCAAACTAACTAATGTGAGCAGCAGCATCCATGGACCATGACTTAAAAATATCTGCAAATGAAGTAAAAATACATTCATAAGTAAAACGAGTTCAATTCTTTATAAAATGTCGGATATCAAAACCGAATATCTTAGAAAGCAACCGTTTCAAATTTCATCTGAAGCAAACAGTCTAGATTCTGCTTTTGATATCCTGCAAATTGTCCAGCCTTTTCGAAAAATCCGTAACTTTTCGTATGTGTTCCATACTCGCACGTGCAAGGATGTTTTGTGCAGGAATAGTGCAGCGTAGACTGAGCATGGGTCTCAATCCAAAGTGAGTAGCGCTTGCACATAACTCCTTCCCTTAAACACCCCATCCGAAATCCC

Amplicon 5'

Amplicon 3'

GGTGGCCGACTACACAAAGAACTCCATCCGCCTGGGCGAGCAGTGGTGCCGCGGCGTGCCCATCGAGGTGGCGCCCATGGCGTACGTGCCCATCAAGCTGCACATCGAGGCGCTTTTCGGCGGCGAGGCCTCGTTGCGTATGGCCAAGGTGAAGGCGGGACCCATTGTCACGGACAATGGCAACTTCCTCCTGGACTGGAAGTTCATCGCCAACAGGGAGTACGACTGGGACGAGGTCAACCGCGCCATCACCCTCATCCCGGGCGTCCTGGAGACGGGCCTGTTCGTGAACATGGCCCACAAATGCTACTACGGCATGGCCAATGGCTCGGTCAAGGTCCAGAACAAGTAGTTCTTGGGCGGAGTTGGGGGAGCGCTTTTTGCATTTATGTTTCACCAAGTTGTTCCTTAAATTTTAGGTTTGCCATTTGTAATGGATTTAAATACATATTGAGTTCTTAGCCGAGAAAACGATGTGTCCTAACTTGAAAGTTTCAGCAAACGAATCTTATTCAGCTTCTTCATTCAAGTATAACATCGAAATACGTCCAAATCACCACAAAAGGGTATCCATTACACTGAAATCAGACCCACTAAATCTCAATTGGTTACAGTTCCACTACAATAAAGTGGATGTCGAATGTCAGTTCACATGCGCTTTGTATACTTTTGTCTCGATTGCGGCTACAAAAATAAATAAACATTAAACAAGCTAGATCTAGAAGGCCTTCTTGGTCCGCCCAGGGAGCAGGGCCTCTTTCAGATACTGGAACAGCGACAGCAGGCCCTGCTTCTTGTTACGCGAGAAGTAGTTGCTGACCACGTCCGGACTGCGCCGGCTCTGGTCGCTGACGGTGGTGGGCAGGACACTGTCATTCGCACTCAGCGAGACGAACAGGGCGAAGGGCACGATCCACATGCAGATGGTAAAGTAAGCCAGCACCTGAAAGTGTAAATTTCCTTTAGGCGTTTGCTGCG

TGCTGCGGCCTAATTCAAGTTGTATGTAAACAAACTGATGATGTGGATACACAATGCAATGCATATTGCATATAATGGCTGTATAAAAACGTACATACGTTTGTACGTAGCGAATGCGCTTTCCATGACTAATGATTTATGCAATACATATGCTCACCTGCGTGAAAGGCACATAGACAGTGACGAAATGCTGGAAGGCCAGGAAGTGATTGACCACCAGCATGGCCAGGGAGCCCAGCAGCGGCAGCGAGAGCAGGCGCACGAAGGGGAATCCACTCATGATGCCCAGGTGGAAGCCCTGCGCCACCAGTCCGCAGATGATAAGGCTCCACGGCAGGTCCTCGAACAGCAGCAGCAGGATGTAGACGAAGATCGTGAAGCTGATCAGAAATAGGATGCACTTCCTAGCCGCCGTCGTGTACTCCTCGGCCAGCTCCGCCAGATAATAGAGACCAGCCACTGGAAATACAAATTGCAGACGTTGCAATTGGACAAGTGCGGCTGCACGGCGACAGCTCACCAATCGACAGGGTGAGGAACACGATCTGTATGGCCAGCGAAACCCATCCCAGCAGGTACAGGACGGCCATCTTGCGTACTTCAGGCGGAATCGAAGGATATATACGGCTCAAATCGGCCGACAAAACAAAACAATCCGATTAGAGTGGCGTGTGGTGTGTGGCTCGAAATACCAAACATTTAATCAGCTTGTATAGAAACGGGCACACTAATGCGCAGAGCTGCCAGATATTCTCGTTCCAACGCTTTTTCAAATATTTTTTGTGGGAAACATGAGGAATTTAGGATTGAAATCCATATGCAAACTAACTAATGTGAGCAGCAGCATCCATGGACCATGACTTAAAAATATCTGCAAATGAAGTAAAAATACATTCATAAGTAAAACGAGTTCAATTCTTTATAAAATGTCGGATATCAAAACCGAATATCTTAGAAAGCAACCGTTTCAAATTTCATCTGAAGCAAACAGTCTAGATTCTGCTTTTGATATCCTGCAAATTGTCCAGCCTTTTCGAAAAATCCGTAACTTTTCGTATGTGTTCCATACTCGCACGTGCAAGGATGTTTTGTGCAGGAATAGTGCAGCGTAGACTGAGCATGGGTCTCAATCCAAAGTGAGTAGCGCTTGCACATAACTCCTTCCCTTAAACACCCCATCCGAAATCCC

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