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CRIMIC CR00893

Gene Ate1

CG9204

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:20256289..20256308	56F11	FBti0202656	81152

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTGTGACCGTTTTGAGGCACAATAGG	AATGATATCGTCATCGGAGACGGTCC

Images

Construct Design Sequences

sgRNA sequence	GTAGCGGGAGACTTATACTCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCATTCATAGGTCTCCGGG	AGTCTCCCGCTACATGTAGA	ACAGGAAACACATAGGCGCA	TTTAATCGGCGAGCACTGGT

 

Amplicon 5'

Amplicon 3'

GCCATTCATAGGTCTCCGGGCACAGCAAGTAAGAGGGGGACAACTTGCCCTTGTAGCGCATTTTGGGACAGGAGTGTATGTAGAAACCCATATAGTAATACTTGAGAGAAGGCACCTTCTCGGCGAGCGACTGCACCAATTCAATTTCCCTGTAAGAATTTCTTCTATGCAGTTTTATCTTTCTTTGCTCGGAATCTTGCTTTACCTGAGTGAACCATATGTGCCAAGCGATAAAAAGCTATAGTCCGGATCGTAGAAAAAGTACACCGAGCTTACACAACCCGGCAGAATGTCAATCACGCCGACGGCAATCAACTTGTCGTCCAGCCAGTACTGCTGATGAAACGAACCGTAGCCCATTTCGGGACCATCCCAGGGTTTCTCATTCTGCAAGCAAAAACGTCCCGGAATTATGAGGTTAGAAGTAAAGCTTTTAAATGTATGAATTATTATAGAGAAAAACCTGTAGGGGAGTCGCCTGCAGATGCTCCTTGTACGCGTCCTGATCCTTTGGCGGATCATTATGTATCGATATCTGATACTTCTTATAGAGCGCAAAACTCTGGGGCAGGGTCCGCCGGAACTCGTCGTCGTAGACGTGAATTAAGCGCAGCTGCGAATACATTTTTGGGGCAAAGATGAATACTCGTTTATAATTACCTTAAGAGGCGATTTGACCAAGAACCGCTGCATACTAGCTAGGGTGAGGCGTGCGGGGTTATCATTATGAACGGTTATTTGGTATTTGCGATACAACGCGATCACTGCATCGGCACAAGTGCGCTCTGTGTCTGAGGAAGCGACTAACACGATCTGCAAGAACACAAACAGCACAAAGACAGATGTGTCTACATGTAGCGGGAGACT

ACAGGAAACACATAGGCGCAATATACAGTGTAAACCCTTATCAACATATGTACATAGGTTCACGCTATCCAGCTTAGTTTTGCAAACGGTCTAACTAATTATTCTATCCACATTTATTCGCAACGCACCTTTAGCCGATGTTTGTTTGTCTCGCTGTCCGTGTTCAAGAAGTCGCGAAGTGTCTTTTCCTGGGTAAGGGATTTCGTTGAGTAGGAGGCCGAGTCTCCCAGCTTGGCCAGCCGTCGATCCAATCGCATCTGCTTGGCCTTCTTGCACGGCTTGTTTGAGATCGGTGCTGCGGCTGATGAAGATAAGAGTGATGCTAATATAATAAATAATATATATATTACTGTGGCATTAAGGCATTAAGGAGCATACGCACATTTGTTGGACCCCAACGTAGGAGCTTCCACCGCGGCAGCAGTTGCCTGCTTCGTCGGCTTCCTCTGAGCCGTTGCCAGTGATGCAACTTGCTCGACATTGATAACAGGGGGACTCTTATCTGGCAGCTGGGGTTGCGGTTCGCTGGCCGTGACCTCGGGAGCCACTATGGCGTAATCGGCATCGGCTTCCCCGTCACCGTCGCCGGCTTCCGGTTTGGACTCTCGCTTGCCGTCGCGCAAAAATCGATTTATGCGGCGAAGTATGCGCTTGTTCGACTTGGAGAGCTTGAACTCCAGGCCATTACATTTGATTGTGTAGCAGGGGCAGCAGGTCTCCTGGTTGCGCAGCTTGTAGCAGTAGTAGCCGCACCGCCGCCAGCCGCGATCGATGAGGTCCTGATAGTCCCGGCAGTCCAGCTGATACGCGTGCATACCTGTCATTTATGACGAATCCACAATAATTGGTACATTGCACCTGGTTTTTGGACTGGTTTTGCATGTTCTCACCGTGCGACAGGCTGCAGTTCGCTCCCGCGCAGTAGCCGCACTTGCTCTGCTGGCTGCCATAGTAACTCACAATGCTCAAGCTCATTATTAAGCTCTTCCGACGGCGTTTATAGTACAAACATATTGCATTTAATTACGACTAAAATATTAAAGCTGCAAGCCGCTCTGTTGCGAAATTTTATATGCAAGCAGTGTGACCAGTGCTCGCCGATTAAA

 

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