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CRIMIC CR00894

Gene CG42360

CG42360

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:24608207..24608212	60D9	FBti0199675	79347

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTGGCTGAGTCCTAATCCTGCTTCA	GCGTTTTCCGTTTTGGGCGTTATACG

Images

Construct Design Sequences

sgRNA sequence	CGTCATGGCACCAGGTCAACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCACATTGCGGTATCAGCTG	CAGGTCAACTGGTTCTCCGG	CATGACGACGAGGCCAACTT	GCTGTCCAACTCTGTGACGA

 

Amplicon 5'

Amplicon 3'

GCACATTGCGGTATCAGCTGTTTCCCATAGCTTTGTTGAGTCGATAAAGATCAGAGTTTTAACCATTTAATGCATTATTCAGAAGCGATTGCCCCGAATGAGCGAAACCAATAAAAACAAGGATCCCACGGGGACCAGTCTGAAGAAGCAACCTTGGCACAAGCGCGGATCGGCCTCTGCAATGGCCTCAAATATCTTGTTGCAGCTGGCGGCCGAGGATCCCCAAAGGGACGCTTATCCTAAGGACACGGTGGTAAGTTGCCTGTTTTATAAATGAAATCCATTTTTGCGACAATATCAAGCCCGCGTCGGCGGCACGCGCAGTTTCTGTTTTCCCGCGAGTGGTAACCAAGTGGTGGTAGGGGCTTCTTTGGGCAGTGCGCAATCGCCAGCGCCCTGCACCCCACATTCCCATCGAATAGTGAAAAGTTCCAGAAAGACCAGCTCCAGCCAACTGAATCACTGCTGCCGAAATCAGCAAGTCCGAATCCAAGAGGCCAAAGTATCACCTACAACCCACCAAGTATGGAGAAGATCTGGAAGAAGGTGTGCGAGCACCACGATGTGCCCGAGCAGGTGGCCAACGAGTGGTTCGCCCGCATTCAGCAGCACCTGAGCTCCGAGGATCCCGCTCGCGCCTACCACAATTGGGAGGAGATGATGCGGCGCAAGGAGCCGCACTTGGCGGGCGTGGCCAATCCCAACATTGTGCTGGCCGCCTTCTTCCAGTACTACCACTTCGATGGTAACCGCAGCTGTGCGGAGAAGAACTGCGAGGTCTTTGAGGAGTTTTGCAATGATGCCGTCATCGAGGATGTGAGTAGGCGTGCGGTGTAACTAAACCTCTATATTCGGCGTGCTCTACCTTAAACAGGACCATGCAAAAAGCCTGGTATGTAATTTGCTGGGGCGCAAGACCCCGGAGAACCAGTTGACCTG

CATGACGACGAGGCCAACTTGCTGCAGGACGTAGACCTGGTGGTCCTAGCGTGAGTATTTGTGCAATCATTGTCCGTGTATTTCAAAACCCATTTACACTACTAAAGTGCTTCGCCGGAGGAGTACAAGCACTATACAACTCTCCTTCGCTCGGAGTACGCCAATCTCGACGATGCCACCTACAAGGCCATGCGCATCAAGGTGCTGGAGACCCTGCTGATGATTCCCTCCATCTACGCCACTGGAGACTACCACGACAAGTACGAAGAGCTAGCCCGCGCCAACATACGCAACGAGATCAGCGAGCTGAAGAAGCAGTAAATCAGAGTTGGAGGACCAGGATGTAACGGGGCTGCTTTGTTTACTGCTGTTGAGTTGTGTACTTACTGGCCGTTTTACCTCGGTTGACTGCAAGGCACTCGTGTATTGGTTGAGCAACCTTCTCTGCCGAACATCTACTTAATTTTACTCCTAAGTGAAGTGTAATCAAAATCATTTTCCTAGTTAGTGTGTACGCCTAGATATATACGTAAGCCCTGTAAACTCCAGCTCAGAATAAAATTGATGTAAATGATGTAACAGAAATGCAGTTTCACTCAAAGCACTTAAAGAATCAATGTATTTGAAATCGCAGTAAGTAGGAAAAGTAACCGTAGAAAAACTTTTCCAAAACTATGATCAAGTCACAAATCAATTAATTACATTCCATTTTTCCATTTATAGATCGCAGCACAAGAAACAGGGGCCTCCAGCTCAAACACCAAAATGCCCAAACAAGCCGTTAAGCACAAGGGCTTCAAGAAGATCCGAGGCCCTAAGCCAAAGGTAAGCAATCGAAATATAAATATATTTGTATTATAGTATGCGATACTCCACAGAACGGCCAGAAAAGACTGAAGCGCAATGCGACTCAGAAGAAGGCACTTAATCGAGAAATGGTATCCAAGTTCCTAAACTCCATTAAAAAAAAGTATGCACTGCAGAAAAATCATCACATTAAGCGCAGGTTGGACTTCCGCAGAAGGGAATCGCCAGAAAAGAAAAACGCCCTGCCCAAGCCGAAGTTCTACAACCCGAATAAGGTAATGCTTTTATAGGTAATATGTTTCAACAATATAACATTCTGGTCAACCGAACATCAGAATGACAACAAAAAAGAAGAAGGAGAAATCGTCACAGAGTTGGACAGC

 

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