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CRIMIC CR00895

Gene Daxx

CG9537

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:6481586..6481616	26D8	FBti0199676	79348

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAGATGTCTGGCGATGACGGTTACT	GGCATTCCAGTGGCTTCTGTTGCTTT

Images

Construct Design Sequences

sgRNA sequence	TGGTTAAGACGCAATTCGCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGAACGAATTGACGACGTCG	CAAACACTCTCCAGGAGCGG	GGAAAGTCGTCTCCGCTGAT	TTGGTGTTGCGCCATTTGAG

 

Amplicon 5'

Amplicon 3'

CGAACGAATTGACGACGTCGACGTGTCATTCAGTGTGGCCGTTGGTGAAACGTCAAAATAAACCGTCGTGTCACTTGGCGACGACCGGTCACACTGGCACTGTTAAGCATTAAAATTTGAAGTTGAGCCATGGGAAAATCTATATTTTTGGGCTGTACTGCAAAGCTGCCTAAAGATTATGTTCCGATAATTAAATACATAAACTAGCAAGAAGATCTAACAATTATATTTTTGGTGGAATTCACGTTACCTATCCAGAGGGTTATTTATGGTTTCTTTTTTGTCTAAGATGGGCCAACATATTTAGCTTTTGACATGTACACATTTGGTTTCAGGCATTATAGGGTTAATAAAAAGGAGTTTCGATATTTAGTTGTGTGTCCATCGCTGGCACCGGCTGGCACCTAGCGACCGATACTATCGTGGCAGCCAGCGATGTTTTAGCAGCTCCAAGACTCTCCAATTTGACAATTCTGAGGCATGAGTACAAACAAACACTCACGCAGTGCACTCTGTTGGCGAGCGTAATAAATATGTGGGAAAATTCCCACATATATACAGTATTCAGTGCTAGTGCCGAGAACTCGCAACGAACCCGGTCGCGTCGTTCACATATGTGTGAAAAAGTAGCTGACAACAACGGTGGCAAGTCCAAATGCGAAGAAAAGCCAGTAAATCGCGCGGCGAGTAAAGTGAATAAATTCGGTGCAGACCATTGCCGTTTCGGGGCGAATAATGTCTGCGTCTGTGATATGCGTCGATTTGAGCTCGGAGTCTGATGAGGAGGTAATATCCCGATTTCCCAGCGGACTCGTCAGTGCGTGTGTGTGTGAAAAAGTACAGTTGGTTTTAAATAGGCTCGCTGCATGAGTATGTGTGCGTGTTACACATAGTGTGGAATTAAATTTTTTTCGCGCTGCTTTTTTATTGAGAACGAGCAGACACGAACGTGCAGTGCCCCACTCATACAGTTGCAAAACGGTGACAGTGCTGCCAGACCGAGCGATTGGAGACCTGGCGAGTCTTGCAGTTGCACTGGCGAATATCGGACTTTAATTACGTTAGGCGTGGAAATGAGCGAGTGAGAGGGTGCGGTGAGTCTCCGCTCCTGGAGAGTGTTTG

GGAAAGTCGTCTCCGCTGATACGGCACTAAATCAAATTAACTCTCCAAAGCGCGCGCACGCAACTCTCCAAAGCTCTCGCACACACCGACGAACGCACACCTATACACTGACGCTTTTCGTTTGTTTATATTTTCATTCAAATTTAAAATACATCTGAGAGCGACCAACAGGTCGCCTGCAGTTTGGAACTATTATAAATGTTGCTAATTATAAAACGCGCTTCAAAGACGATGCACTTGTGATAAAAACAACAAACATTATGTATACTTATAAGTAAACTTTGGTCTTATTGTCAGAGGCAGTGTAGTCTCATCTTATTACCAATTGAACATAACTTGTAATATAAGTTTCAAATTATATTTGCAGTCGCCGGCCAAACGACGACGACTCGAGGATCCGCTGCGACTACTAACGCCATCCGGTAGGCAAAGTTTTCCTGCCAAACTTCTGAACATACCAAAGGCCTCCCTGGGATCCGCGATGGTAGTGCCTGTTGCGGAAACAGGACCTGCAAGGGAGACTCCGGTCATAGTGGATCCACTCCGGTCCATGCACATCCCCAGTGGAATCACCATCACTAAGCACCAGAAGAACGTCCTCGCCACAAACGGAAACATGAACCTTTCTCTGGCAGAGAGCAACAACAACAACAACACTACCAGCTTGAACAAAAATGTGACCCCGAAGCAGATCAATGTTGGCACCGGCCAGAAAGTGGCCTGGTCGGTGAGCAGCAAGGCGCCCAGTCCGAATGCCGTGATAACCACCGTGCCCAGCCACCAGGTGAGGGTGACGCCGTTTAATCCGATTCGACTCGCACCCGGGTCAAAAATAATACCGGCTTCGAATGCGATGCCTGCAAAGATTCAACCCATTCTTGGGATGGGTCAGCCTCAGCAACAGGCACAAGCCCAGCAACCGAAGGCCATCATAGTGGGTTTTCCGAAGCCCAATACTGCAGTGGCTCAAATGGCGCAACACCAA

 

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