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CRIMIC CR00896

Gene CG7220

CG7220

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:10736096..10736096	47B9	FBti0199677	79349

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTAAGTGGTTGTCGATCTGTCGCTT	ACGTACCGTGGTACCACCATTTAGTC

Images

Construct Design Sequences

sgRNA sequence	AGTTGTTCGAACGCGATATCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGTGCCCGACAGTACGTAC	ACGCGATATCTGGCCAAAGT	CGCGTTCGAACAACTGGTC	CTCCGCCTCGAAGTCCATAC

 

Amplicon 5'

Amplicon 3'

TTGTGCCCGACAGTACGTACACAGAGACACGTCGCTACATCGGGCTTCGTCACTCGACTCGCCCTGCAGTCTCTCCCACAATTTTCTGGGCGCTTTCCTGCAGTCGTCCCACGAACGTTGCCGCAATTGGTCTACACGAATCGGTTCGCTAATAAAATGACTGCCATGCCCCACGCCCACTCTCGCCTCCTAGGAAAAACGCTGGCATATTGAACTGTGCGACTAACTAACTTAAATACTTAAAAGCAAGCCCAGCAAGCCGAGAGCAATCTGCCTAAAAGTGACAAGTTATAGGTGACACAACAAAAGCTAAAATTTGTGAGAAACTGTGTGAAAACTAGAACTACAAAATGATGAAGCTGTTTAAAAAACCCAAAGACAAAATACCCAAATCGGAGATTTTAACCGAGCCCAAGGAGCCCAAAACACCGCCGGTCTCCAAGTGCGGAAAACCTTTGCAGTTGGACAACTCGCGATGGGAGGCAAGTATATTCGAGATATTAAACCTGAACTTTGGCCAGATATCGCGT

CGCGTTCGAACAACTGGTCGTTAATATACATATAGTGTTCCATTGCATCATGAGTATGTAGTATTTTGAGTTTCAAGAAATCTGCGTGAATCTTCTGTTGATTCTCTTCAAGGCTGTACCAAGATTTGTTTTTATTATTCTTGGCATTGCCAAATCCAAACCTTATCGATCAAACCCATTTATCCGCAGCTCATTTGTGGGGGCAGCGGTGCGATACTTGTTTATGGTACAGAAAACGGGCCTGCAAGTGCCAATCACTCGATAAGAAGGGGGATCCGAAAGACGGAAAAACAATCGTCGGTTCTATAAATTTCAGTGACATTTGTTTTTATCTTATCGAGCATTTCGTTTATTTATACGCGCGTAGGCATATAGCGTATATTGGCGTTTGTAAGCCAAAGGAGAAATGGCGCACCGATAAGGCGAGCGGAAAAAATAAGTCTTGAGAACTCTTAAGTAGAATGTCCCAGCTGTAGGTGTGTTTCGCGATAAGATATACGGCGAACTGTCCGAGTCATTAACTAATATAAGTCTTAAATTTTATTTTCTGCCCCACAGCGCCGCCTACACAAAGAGCTGATGTCCCTGATCAAGGAGCCGCCACCAGGCGTAACCATCGACACCGAGAGCGTACAACAAAATTTATCCGAGTAAGTATGGACTTCGAGGCGGAG

 

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