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CRIMIC CR00897

Gene CG13229

CG13229

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:11142646..11142669	47D5	FBti0199678	79350

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGACAGACATTGGATTGACTGGCAGT	ACAGAGAAGGTCATAGCCGATTGCCT

Images

Construct Design Sequences

sgRNA sequence	TCGTGATTACAACTCCCCCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACCGCCAGCCACTTAAGTA	GAAACCCGCAATGTTGGCC	ACGAGTATTACTTACCATCAGCG	TTCGGCCAACTGAGTGATCC

 

Amplicon 5'

Amplicon 3'

CACCGCCAGCCACTTAAGTATATTATTTATGGGCGTCTTGGCCATTTCCTTTCTGGTCAGGACCATTATGTTCAAGATGTTTGCTATTGTCCCGAAAATGCAAATCTAGGTGATTTAAAGAAACAAGAAAGAATCAACATAAAATGCGTCCTTTGTCAAATCGAAACTGTGAGCGCTCACATACTTTCAACTGAATGCACTCCCCTTGGACACCAAAGTGGATGAATATTTTATTTATTCAGCTCGTGAGCAGCAACTCAACTGTCGTGACCTCTCGTATTTACATTTCTTAACATTGCTCACACAAGCAAAAAAAAAAAAAAATGGAACTACAGTAACCGAAGTTTGGTTCAGAATTTTATTTCAATTTAAGAGGTAAACTATTTTTTGTGCGAACGAAGGAAAGCAATAAAGTTACATTTTTCAACTGTATTTGATTTATTGTATATTTATTTGAAAAGATGGGCTATGAACTAGCCTTGATGGAAATGGATTCTCGGTATTTTCTGACCCAATTCCTGTGGTAAAGGCCGTCTGCTACAACGCAAAATGACTTACGACTGAAAATTATAATGAAAAAAGCACATTAACGCCTCAACGGCTTAACTACATCTAATATAATTTATATCCTTTTCAAAAAAGCCCTGAAAAGGGAAACCCGGACATAAACAAAAGACCGAGATTCCCGGCCACTAATTTCATTTGTGATTTTGATGGTTAAGCTGAGCGCATGCTATAACTTTAATTCGAAAAAAGGACGACTTTGGCAAGAGTTCAAAGGTAAAGAAGTCCCCTACATTCATCAGTGGTCTGTCGGCGAAGGTCTGTGTGTATCGTCTTGGCTTCCCTTGCTCATCATTAGTCAACCGATTCCTTTGTTTTGGCCAACATTGCGGGTTTC

ACGAGTATTACTTACCATCAGCGAAACGTATCCATGGATGCTGTTGTAGGCTCGCAACCAGTTGTATATTTCCCCCTGGCAATAGCCCTGGCACTCATCCACCTGAGGGGTCATGTTCTTCATCTGGTACTCCTGCCATAGCTTTACAAACTTCTCGATATCGGTGCTCGTGAGATGATAGTCCTTCAGAACCGTGTCGTTGAAAGGAACCCGCTGGTGCATATGGGTCTGTCCCATTTGATTGCCGGTCTCTTGCATCATTGTAGACTATCTAGTTCCGCTCTTTTGATGTATATGTGCTAGTTAAAAACTGGAACAAAATTTAAGGAGTTTAAATACATAAACCATTGCTTAGAATTATTTATTTAGAATTAGAATTATATATTTATTTGAATTTTAAAGGACTTCTTGTCATCGAAATGGAAAGTTTTTCTGTGCTTTACAACTAACTTGTGTTTTACAGGACCATTAAAGTGAAAATGCCATAATGTTTTACAAAATAAAAGGGAAATTGTGTCATTTATGTATAAAATCAATACTATCATCAAAAATGAATTAGACGTCAACAGCATCTAATTTATCATTACTTTGTTATTATCAATGCCGTTTCTTCATTATGGGCATACGGTGTCGAAATTGCTACTGCAACTCGTCAAAGCGATAAACAATTTTGAATGGCCAAAGTGGAGCTGCCAGTGAGCTGAGCAAACCTGTCCAAATGTCCACCTGTGATTCAAAGCAACCTGGGCGTTGTAGGCAATTAATTGGCAAATGCGGACCCATTAGTCTGTGTATCTATTTGCAAGAACTTCACATTTTGCATTTGAGAATATGGTTAAAATTGCTAGGAGATCCAAGTAAAAAATTCTTTAGATACTTTATTATATTATATTATAATTACATTTTAATGGTAAATCCATGTTCGTCTTTCTGTCCGTATGAATGTCGTGATGTCATAGAATTATTATTGCTTGAAAGTTGATCATTATCATGCATGTCATGCAGCCCAATTATACATTACTACACATGTAAATCCTTTATTTATTTCTTTCAGTGCATAAGTAATGTTGGCATCTGACACTACTACCCCGGATCACTCAGTTGGCCGAA

 

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