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CRIMIC CR00898

Gene Arpc2

CG10954

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:20417890..20417890	38C6	FBti0202657	81153

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	TCGTACAGGATCGGGTGTCTCTCTAT	TTGTGCAGTTGGCAACCATCTTAGGC

Images

Construct Design Sequences

sgRNA sequence	AATACGCTGTCGACGTAAGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTGCAAGTACTGGCGATTG	ATACGCTGTCGACGTAAGGG	ACGTCGACAGCGTATTAGTT	GTCTCCCGAGGCATTATTGGT

 

Amplicon 5'

Amplicon 3'

GCTGCAAGTACTGGCGATTGGAAATTGGAGGCGCGAAAAGAGGATAAGGTGGATTTTCTTGCAACTGTGTGTGTGAGCATTGGAATTCGCCCGTGTGTGTGGCGAGAGTGCGTCCATGTGTGTGTGACGGACCAAAAAAGCCGACATCAGCGCCCATTTTTTGTTGTTGATTCATTAATACGCCCGTCATGCACCAGTATTGGTGAGTTGGAGCAGCGCACCGTCTTACTCGCACTCAACGGAGCGCAGAAAGAGCGCAGGGAGCGGCAGAAGAAGAAGCAGCGAGCAGCAGCACCATTAGCGGCAGTCAACGTCATATAATTTGTCATCGAAATTGGCACACTAAAAATAACAGTTAACTTCAACCGCCATGATCCTGCTGGAAATCAATAATCGGATTATCGAGGAGACGCTGCTGGTCAAATACCGTAATGCCCAGGCTGGGTAAGTCAATGCCTTCCAGTCCCGTTTCCCCAATTTCCATTCCACCATTTTGATTTCTATGCTGCATTCCCCACCGTTCTGTTATCACCAGTCGGCTTTGTTATCAATTTCGTTAGCTCTCTCTTTCCTCGGGAATAAATTTGGGTGTAGTTCCAGCCTTGGAACACTCATCGCTGCAGCTCTCTCCCTCCCACACACGTACCCAAGTACAGTGCAGCCCCCTTACGTCGACAGCGTAT

ACGTCGACAGCGTATTAGTTAATTCATTACAACTCGCTATTCGCATCATCGGTGCTGAAAATCTGCAAAACTGACTCATCCATTGACGTGCATTCAAACGCATACGCTTGTGATGATCGAAAATATTAGTATTTATTTGTTCCTAATCCCGAAAAAGGATATTATTATTGAAGAAACATTATCAAAGGAGTCGTGTTACGAAAGAAATATGTTTAAGCTAAACGGTTTAGAAGAAGCTTAGACGAAGCTTACAGACTAACAAAATTATTAAAATTAGTAAAATAACTTATTGTACTAACAGCACTATCAGCAGAAAGAGGTATTTGTGTTTGGTAGCGAATGATTAAGATCTCACATAAATAAATGTATTTAAATGCTAATAGTTATGATGCCATTAGTTTATTCCCGATAAGATTGCAGTGTAATGCCTGAAATTAAGGCAATAAAAGTTATAAATTGTGGAACAAAAGTGGCATACTTGGAAAAAATCTCCTGTTGTGTCTCAAATGTTCCAAAATAAACCAAAAGGACGAACGGCATCCTGAATAATCAATCATATTATAGCAGATGACTTGTGTTCGTGTACATAAAAACTAATAGTTTCAATATTACTCCCCTATTATCTGTATTCTTGGGAGACAAAGTTTACATGCTAAAGATTCTCCATTTTCCGTGTCTACAACGCTCCGATATAAATTGTAACGCTTAAATCATCTTGAGTAATTCTTTTTGTTTCTTAAGATAAATACTTTCGTGTACATTATTGCAAATTTTAGGCTTTGGAGCCAAAGTGGCATTCTTCAAAAACAATATTTGATCTGTGTCTCAAAAGCTCCAACATTAACTAGAAAGACGAACGGCATCCTGAATAATCAATCATATTATAACAGATGACTTGTGTTCGTGTACATTTGCCATAAAATGTTCAACTTTTGTAATATTCTGGAGTCAAAGTGGCACGTCTTACGATCTTCCTGTAACTTGTGTCTCAAATGCTCCAATATCAAACGAAAGGACGAACGGCATCCTGAATAATCAATCATATTATAGCAGATGACTTGTGTTCGACTACAATAGTATTCGATTTATTAGACCAATAATGCCTCGGGAGAC

 

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