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CRIMIC CR00901

Gene Nha1

CG10806

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:6863993..6863993	27C1	FBti0206845	83199

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no rxn	no rxn	ACCTATGCGTATCATCGACCGCATAG	GGCAGAAGTTGTACATCCAAGACTCC

Images

Construct Design Sequences

sgRNA sequence	ATAGCGATCATTCCGCTTATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAAGTGGCGCCCAATAGTCG	CGATAGCGATCATTCCGCT	GCCGGCGGTTAATGAATTGA	CAAGTTCACAAGGGCACTGC

 

Amplicon 5'

Amplicon 3'

TAAGTGGCGCCCAATAGTCGAAAATACTTTGTTGAATTTTTTAAAATATATATAATAACACTTGCCTTAATTACAATTTAGCACGTTTGCAGAAGAATTTAATACTTCATTGAGACGCCTATGATTGAGTTTTTGAAACGTGCTAGTAGCGAAAATGCTCAGCACACGTGAGAAGTGAGGTGTAGACAATATACGTCGCAGACTATATAAGACATATTTTTGAAATATAAAAGTATTGCGCAGATATATAGCTAATAACCACACAGTTACTTATTTGATATTAATTTATAACAAAACTCTCGCTGACAAACTGCTTTCCTAAGACAAGGATTCTGCGATCCTGGACAACATCACTTACCATAGCTGTGAGTCGAGACCCGATCACTTTCGTTGTCATGGGGAGCGGTATCCTTCAGGATGCTCTTCCTTCGCGCCGGTCCAATGTCCGTGTGCGAATGTGTGGAAGTCTGCGATGGTAATACAAATCGCGATTAATTTTGTTAAATAACAATACGTTATCGCTGGCACTGGTTCACAAGCGTGTGACCATATATACATATATTTTCATTAGTCGATTATATGGCCAGTAGTACATGGAAAATCCGTTTTTACGATGCAACCAATAAGCGGAATGATCGCTATCG

GCCGGCGGTTAATGAATTGATATAGCCAACAGCACTCACCTGTGAAATTTTACGCCTCGGATTTTGCTCATAGGCGGGATTATCGTATCCGGTGCCGAGACCACCATTCCCACCGATCATGGGAGGATCGGAAATAATGCTAACCCGTCGCTTGGGTTGGCTCTCGATGACGATGGTGCTCCTGTGGTTGCCAGCCCCATTGCCACTGCCATTCGAATTGGGCATGGGCACATCGCTGGGCACTGGGATCGGAGTCGGAGCGATGATGGTCGTCGGTGTGGTTATGGTCGCTGGTGCTCCGCCGACGGACGGATCCTCGCGCGCTTCTGGCTCTCCCGTGGCCGTCATCTTCTTATGGCTCTGCCGGAACGAAATTTACAATAAGTTAATCATTTAATGCTTCGATTAGTTTGCCTTTGCGCTTACACTAAACATTTGACGGCGTCGCGAATCAAATGTATTCAATTATTTCGAAGCGATTCGCGAGTTTTTACAACTATCTCGTTCGACGTTTAGCAATGAAATAAATTCATTACCACTCGGTGGCAAACCGCCAACTCCAGGTGGACACACACACTCATAAGGGCACACACACACACATACACATACAGGCATCTGGTGGCTACACTGGGCGAAATGGCAAGGCGCTAATTGAAAAGCCAAACAGCCATTTATCAAAGCCCAGGAGAATGACAGGTAATGTGATATAAAGCGTAATTGTGGGAATTAATGCCCTATTTCTGCAATTCATTTATTTATTTATAAACTACTCTGAAAATTAACTAATAAAATATTTAACATTGTCATATAAATATTATGAGAATTATTGTCCTCTTTCTTTAATTTATTTATTCATTTATAAGCTACTATAAACATGTACTGTATTTTATTGCGATTGTGAGTAGTTATGGGAAAAATATTTTTTTAGCTTAGAAAAGTATATGAACACTATTTTTTGATTAAGAATTGAAAAAAAATGGCACATAATTAACTGATTTCGTAGATTACGTATACGCACTGTATGTCTTTTGAGAACAGACATAAATAGGAAGAAAATATATTGCTTTATCTAATGCGTGATTTTTTCTGAACATATATGGTTATAATCTTTAAAAATATCTTAAATATAAAATATTTTTGTTATTATTTGATTTATTTATTTTTCGCAGTGCCCTTGTGAACTTG

 

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