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CRIMIC CR00902

Gene CG15099

CG15099

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:18845594..18845628	55F7	FBti0199679	79351

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCGCCAACACTTGGTTATCGCGGAT	TTTTCTTGCATGGAGTTCGCCTGCTG

Images

Construct Design Sequences

sgRNA sequence	ATTAAAAACAAGTCGAGATAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGTGAGTTTTTGGCCACCT	TTGTGCCCTCCTCTTTCGAC	TCAATGGGTTCCCGGAAACT	TTTGTCTGTCCCGTCACGAG

 

Amplicon 5'

Amplicon 3'

TGGTGAGTTTTTGGCCACCTCGCTGCCCAAAAGCCAGCTGAACAATCTTCTATTCAGCGACATGTCCCGCCGCAGTATAGTATTGAGTCCATTGGTGACTAGTTTCACTAAATCATCCTCGGAGAGCAGCACCGTATGCATGGGAAAACCGAGCAGTAGGAACTCCAGGGTGTTTCGCTGGACCAGGATCAAGGAGTCATTTAGGCAGACACACAAAGCTGACATCATGATCTCTCTGTTGTGCCCCATAATGTAGATCTGCTCCTGCATGTCCAATCGCTTGTTGAAATGCTCCAGCAGGTAGGTGATGGCCGGCAGTCGAATTGCCGCATTGTTGGCCACCGATTCCCAAAGAACTGTGTAAAAGTGCATGGGATTGACGGCATTGCAGACTTGTTTTAGTAAAGCGCTAATTCGCTCGAAGTGATCCAGACCGCAGTCGTAGCCAGGTAGCACTCCGTTCAAGAAACCGCTTAGAGCGGGCCTCAGTTTGTCACCCAGGGGTACGAAATAGGTTTCATAGATGGCCAACAAGGCGGGTCGTACATTCATGGCCGCATATCCAAGGAGCGGAAATAGCCCAGCGCTGCAGGCAAAAAATACATATTGTTAAGAAAACAAACGAAGTTATCTCGTTCACTGACCTATATATAAACTCCGTGGCCAGGCGCTCCGGTCCTGTTTTGCTGAATATCACCGAATAGGTCTCCAGGGCTTTCAGATGCACCCCGGAGGGCAGGGCGGGATGCATGCATTGGGCCAATCGCTTGGCTATCTTGAGTCGCCGGGGTATGACCTGGTACTGCGTATTGCTCGATATCGCCTGTCGAAAGAGGAGGGCACAA

TCAATGGGTTCCCGGAAACTGCTGTGAGTCAGTTGCTCCGACGTTTATCTGTATTGCCACAGATGGAACCATGAGTGTTATACCTTGCTGAGCTTGCCCAGAGCCGATATCAAATCCGCCCATTCGCTGGAGTACTCGAAATTGCGCAGCGCCTTGTCGATATTGGTCATATATGTGCGGTACTTGGCCTCGGCCATAAGCTTCTGCTCCTCGACGAGTGCGTCCGCGGATTCCATGGCGACGTCTTTTGCCAGTGGGCTAGCTGGCTCTAAATGTTGCTTTTTGCTTGAGTTCGATGATTTCTTGATGGGTTGGCTTTGTGGCAGAGCTCTCTGTGAATAATCAATGGCAATCAATTAGCAAACACTTGTTGCCCGCGGCGCTGCGTCCTTGAATTCCAACTCCCCCACACAAATGGGTGATTTTCTTTTTTTAGTTCACTAAAAGTTTAATGCTTTGCATATTGGAGCAGTGTGGCAGCCGAAGCACCTGTGAAAAACTGGCGATTCTTTCGCCTAATTGGAGGGTCTGTCAATTGACCCTACATTGAGTGTATATATATTGGAAGCCTCGACGCGCTTGACTGCAAAATCGGGTTGAAAGTGATGTCGCGTGGCAGGAACAAAATAAATTAATTTTTGAATAAATTTCAACTCTCTCTCTTTCTTTCTCCTTCTCTCACCAGTGAAGTGCAGTTAGAGCAAAGAAGAGAGGGGACCAGCTGATGACGAATATTCTGCTGTTTTCATGAAAGCAGGTTGGAAGGGGTGGGGGCTCGTGACGGGACAGACAAA

 

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