CR00903

CRIMIC CR00903

Gene CG8735

CG8735

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8599759..8599790	44D4	FBti0199680	79352

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGAGGGTGGTAATGGATTTTCAACC	AGTAATCATAACCCACCGTTGTGGGC

Images

Construct Design Sequences

sgRNA sequence	ATTCGCTGTATACTACGTTCGGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCTTCTTGTCGCCAAAACG	GCGAATATACTAAGAGCCCCA	TGGGCCGCTGCTTATACG	GCGGAAGAAAGCGTAGTCCT

Amplicon 5'	Amplicon 3'
TGCTTCTTGTCGCCAAAACGCTCCAGGAGATTCACCGCCACCTGGGAAATGCATAATGGTACATGATATAGTTTGAAGTATCTAAACAGAGTGCATATAAAAACAGGCGCGAACTCGACTTTGTGAAAGTCGATAAATAGCTTTTGCGTTCTAGTCTGATTTGCATTGATATGAGTTCTTTAAAACAGCTGTGCCCTTTCTATGGTTTACACTACGCATCTTAGCCGAAAATAAAGAGAATATTCTAAGCTCTGATAAGGACTCTGCACTCGGGCATGTAATCAAGGGGAAGTTGTGACCCCACCTTATAGGTCTCTTTGTCCATGACCTGCTCCAATATCTTGCGCTTGTCCTCCTTCAGACGAGTCAGCTTATCGCCATTTTTGTTGAGCTTGCGCTGGAAGTACCACGTAAACAGCCGCCGCAGAAAGATGATGCTAGAGCCCGGGCGAGAAAAAGGTGGGTCAGGAACCGGATTTAGAGCGGATTAGACGGCAGCACTCACACAATGGGGAATAGCAGCAGCGGTACCAGGTACATGAAGCACTCCTGCATGGTCGGCGGGAAGTAGAAGTACCACCAGAGCCCGAAGCCCACGATATATGCTCCGATGGTGAAGCCCACGAAGTTCGTCACGAACCGGCGCTTGCGCTCCTCGGTGTTGATCATGTACTTCTCCAACGCTTGGATCTGCGTCTGCAGCCCCTCCAACACCGCCTCCGTGGACTTTTCTTTCTATAATTAGATTCGCGAATTCACGACACATATTTTTTGGGGCTCTTAGTATATTCGC	TGGGCCGCTGCTTATACGCACCCGCAGCTTGGATAACACAAATCCCATGGTGTATGATCCGCAATTTAACTAGAAACGAACAAACTTAGACATTTACTTGACTATTTTGCTATTTAACCGCCCGCTTGCCTAGATATTATTTGCGCTCAGCGTGACCGTGCCTGTACCTTTCGAATACACCGCGGGAACCCACCAAAAATACCAAATTCCTACAGTGCTATCGCGGACTCAAACCACGAGAGAATGTCCAATTGGAAATGTCCGCGTCTGTTCAAATTTCAAACTTTAAATATTTCATATTCTTATATGAAACCAATTTGAACGATTGATTATATTCATAATGGAACATTAAGTACTGAAGGTAAAACAAATTTACGAAGCATTGGGGCCTGAAGAAAATGAGTGTTAGAGTCGCCATTATTCACATGCCTTTATACTTAAAAACTATTTTTGTGGAACAATACGCCTTTTAACAAATTGTTGTTACTGTTAAGTTATCAACTATCTTTAAAGAGCCTACAAATATTTCAGCTCATTTCATTTGAAAATATTGCACTATCGTTAACAGAAGCGCATTGTATTTGGCCACCATCGAGCGATGGCCACACTGCCACTGCCTCGGCCACACTGCAGTTCGGATTTGCCGGTTGTTTGGATCGCAGATTGAATCGGAAAGCCAGCGGCGTTGGTTCGGAAAAGTGCAGTCTCCGCGTCGCGAGTGCGCCAATAACGAGAAATAACGAGAATTGAAAAAGTTCCTTAAGAGGTGGAGTCCAGTAAAGCGCAGCAGCAATGATGCCGCTGCAGGAGTCGCCGTCGCCCAGTTGGCAGCTGGAGGACTACGCTTTCTTCCGC

Amplicon 5'

Amplicon 3'

TGCTTCTTGTCGCCAAAACGCTCCAGGAGATTCACCGCCACCTGGGAAATGCATAATGGTACATGATATAGTTTGAAGTATCTAAACAGAGTGCATATAAAAACAGGCGCGAACTCGACTTTGTGAAAGTCGATAAATAGCTTTTGCGTTCTAGTCTGATTTGCATTGATATGAGTTCTTTAAAACAGCTGTGCCCTTTCTATGGTTTACACTACGCATCTTAGCCGAAAATAAAGAGAATATTCTAAGCTCTGATAAGGACTCTGCACTCGGGCATGTAATCAAGGGGAAGTTGTGACCCCACCTTATAGGTCTCTTTGTCCATGACCTGCTCCAATATCTTGCGCTTGTCCTCCTTCAGACGAGTCAGCTTATCGCCATTTTTGTTGAGCTTGCGCTGGAAGTACCACGTAAACAGCCGCCGCAGAAAGATGATGCTAGAGCCCGGGCGAGAAAAAGGTGGGTCAGGAACCGGATTTAGAGCGGATTAGACGGCAGCACTCACACAATGGGGAATAGCAGCAGCGGTACCAGGTACATGAAGCACTCCTGCATGGTCGGCGGGAAGTAGAAGTACCACCAGAGCCCGAAGCCCACGATATATGCTCCGATGGTGAAGCCCACGAAGTTCGTCACGAACCGGCGCTTGCGCTCCTCGGTGTTGATCATGTACTTCTCCAACGCTTGGATCTGCGTCTGCAGCCCCTCCAACACCGCCTCCGTGGACTTTTCTTTCTATAATTAGATTCGCGAATTCACGACACATATTTTTTGGGGCTCTTAGTATATTCGC

TGGGCCGCTGCTTATACGCACCCGCAGCTTGGATAACACAAATCCCATGGTGTATGATCCGCAATTTAACTAGAAACGAACAAACTTAGACATTTACTTGACTATTTTGCTATTTAACCGCCCGCTTGCCTAGATATTATTTGCGCTCAGCGTGACCGTGCCTGTACCTTTCGAATACACCGCGGGAACCCACCAAAAATACCAAATTCCTACAGTGCTATCGCGGACTCAAACCACGAGAGAATGTCCAATTGGAAATGTCCGCGTCTGTTCAAATTTCAAACTTTAAATATTTCATATTCTTATATGAAACCAATTTGAACGATTGATTATATTCATAATGGAACATTAAGTACTGAAGGTAAAACAAATTTACGAAGCATTGGGGCCTGAAGAAAATGAGTGTTAGAGTCGCCATTATTCACATGCCTTTATACTTAAAAACTATTTTTGTGGAACAATACGCCTTTTAACAAATTGTTGTTACTGTTAAGTTATCAACTATCTTTAAAGAGCCTACAAATATTTCAGCTCATTTCATTTGAAAATATTGCACTATCGTTAACAGAAGCGCATTGTATTTGGCCACCATCGAGCGATGGCCACACTGCCACTGCCTCGGCCACACTGCAGTTCGGATTTGCCGGTTGTTTGGATCGCAGATTGAATCGGAAAGCCAGCGGCGTTGGTTCGGAAAAGTGCAGTCTCCGCGTCGCGAGTGCGCCAATAACGAGAAATAACGAGAATTGAAAAAGTTCCTTAAGAGGTGGAGTCCAGTAAAGCGCAGCAGCAATGATGCCGCTGCAGGAGTCGCCGTCGCCCAGTTGGCAGCTGGAGGACTACGCTTTCTTCCGC

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