CR00904

CRIMIC CR00904

Gene CG14763

CG14763

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:7967492..7967492	43F9	FBti0199681	79353

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGCACGAGACGAGACGAGATGCTAT	CCGATCAATATCCGTCTTCCATCCGA

Images

Construct Design Sequences

sgRNA sequence	AAGTCACGCCATTTCTTGTGGGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATCCGAGCAGTTCACCCAG	GTCACGCCATTTCTTGTGGG	AGAAATGGCGTGACTTCGTC	TGTGATCATGGGCGACTCAC

Amplicon 5'	Amplicon 3'
AATCCGAGCAGTTCACCCAGTGCATGTGCTGTGTATGCGTGTGTTCGCAAATCTATTATTTATATATATATATATATATCAATCGTAATATGTACACTCAAAGGTAGTAATAACGATCAAACGCAGTTGTCTTGCATCATTAGACGGATGTTCGAAAGTAATTTATGCTTCAACAAAATATTATATCAAACTATTGTACTGATATCCAAATAAACGACAACTATCGCATCCTACGTGTTAAAAATGTGAGTTGTGAGTTACAGTGCAAGCAAGTGTTTAAGAAAGTGAACCATCTGCTGACCAAAGAAGGTAACGAAATTAAGCACCATTGCTAGCATCAACATCAATTTTATTACCAAGTGGAACGCGCCAGGGCCCTGGAATCGCGTTCTTAATCGTAGTACAGGTAAAAGGTGGTGCAGACTGCAAAGTACGAGGGTCTCTCCACGCTGTAGGTCACAAATCCGTCTTTTTCAGCATCCCACAGGAAGTTGACCATGGAGTAGAGGCCCTGCATCAGGAACTCGCCCACAGTGACCAGCACTATGTAGCGATATCTGGGGCGAAACGGTGGTTAGTTAGACAAAAACTGACCGCCGAACCAAAGTCACCTGTCGTAGTTGTCGAGTTTGATGCGGTTCTTGATTTCCTCGCTCACTTGCGCGGCCATGTGGAGGGAATGCTTGGGATGGAACATGTACTCATCGTTGTAGTGTCGGTTCATCACCGCCTTTATGATGTTCTCCACGCGTTCCTTGTTGAGCGGATTCTTGGACTCCAGGCGGTAGGTGGGCATGAACCGCATGGTGGGCTAGGCATTAAGCAGATCTCAGGGTATAAACACAGCTAGCTGGGCATTTAACGGACTTACCTTGGCTGCCGGAGCACCCATCGGCAAGGAAACGCCCATATGGGACTTGGTCAGCGATCCGCGCTGGCTGCTTGCCGGCCTCTGCATGTCGATGAAATGGGAAACCGAATATGCATGTTAGTTAGGCCACAGCTAGAAGCACAGAGATACCCAAACCCCCAATAGAATCCCCACAAGAAATGGCGTGAC	AGAAATGGCGTGACTTCGTCAAATTTTAGACGAACCGTATGGTTCCCGTGTTCACATCCCGTTACAAGCATCAATTCATTAAAAAGACATCAATACCCCATTGTAACCATAATGGCAAAATCAATCGTATACCTTCTCACGCTCCTGGCCCAGATAAAGATTCACCAGCCCCGACCAGTTCTTCGCTGCAGCGGCTGCCATTCCTTGCGAATTAATAGAATCGAGTCGGCTCGAGTGCGTAGAGGTTAGCCATAAAACCACAAATGATTATGCCATTAAGTCATTCCCTGCCAGATTACCTGACGGCCTGTCGCCGTGGCAATTCGTGGCGTTTGGCTTTGTTGAATCAATAAAACCAGCCAAATTATGCGCCACTTGTCAGCCCAACTTACCGGCTTAGTGCCCTCGGCCGACTTTTCTTTCTTCTCCTTTTTGCCCTTGCCAGTTTCCGCCTCCTTGGCGGCGGTCTCTGGCACGACATCCTTGCTGCGCCGCTCTTCCGGTGAGTCGCCCATGATCACA

Amplicon 5'

Amplicon 3'

AATCCGAGCAGTTCACCCAGTGCATGTGCTGTGTATGCGTGTGTTCGCAAATCTATTATTTATATATATATATATATATCAATCGTAATATGTACACTCAAAGGTAGTAATAACGATCAAACGCAGTTGTCTTGCATCATTAGACGGATGTTCGAAAGTAATTTATGCTTCAACAAAATATTATATCAAACTATTGTACTGATATCCAAATAAACGACAACTATCGCATCCTACGTGTTAAAAATGTGAGTTGTGAGTTACAGTGCAAGCAAGTGTTTAAGAAAGTGAACCATCTGCTGACCAAAGAAGGTAACGAAATTAAGCACCATTGCTAGCATCAACATCAATTTTATTACCAAGTGGAACGCGCCAGGGCCCTGGAATCGCGTTCTTAATCGTAGTACAGGTAAAAGGTGGTGCAGACTGCAAAGTACGAGGGTCTCTCCACGCTGTAGGTCACAAATCCGTCTTTTTCAGCATCCCACAGGAAGTTGACCATGGAGTAGAGGCCCTGCATCAGGAACTCGCCCACAGTGACCAGCACTATGTAGCGATATCTGGGGCGAAACGGTGGTTAGTTAGACAAAAACTGACCGCCGAACCAAAGTCACCTGTCGTAGTTGTCGAGTTTGATGCGGTTCTTGATTTCCTCGCTCACTTGCGCGGCCATGTGGAGGGAATGCTTGGGATGGAACATGTACTCATCGTTGTAGTGTCGGTTCATCACCGCCTTTATGATGTTCTCCACGCGTTCCTTGTTGAGCGGATTCTTGGACTCCAGGCGGTAGGTGGGCATGAACCGCATGGTGGGCTAGGCATTAAGCAGATCTCAGGGTATAAACACAGCTAGCTGGGCATTTAACGGACTTACCTTGGCTGCCGGAGCACCCATCGGCAAGGAAACGCCCATATGGGACTTGGTCAGCGATCCGCGCTGGCTGCTTGCCGGCCTCTGCATGTCGATGAAATGGGAAACCGAATATGCATGTTAGTTAGGCCACAGCTAGAAGCACAGAGATACCCAAACCCCCAATAGAATCCCCACAAGAAATGGCGTGAC

AGAAATGGCGTGACTTCGTCAAATTTTAGACGAACCGTATGGTTCCCGTGTTCACATCCCGTTACAAGCATCAATTCATTAAAAAGACATCAATACCCCATTGTAACCATAATGGCAAAATCAATCGTATACCTTCTCACGCTCCTGGCCCAGATAAAGATTCACCAGCCCCGACCAGTTCTTCGCTGCAGCGGCTGCCATTCCTTGCGAATTAATAGAATCGAGTCGGCTCGAGTGCGTAGAGGTTAGCCATAAAACCACAAATGATTATGCCATTAAGTCATTCCCTGCCAGATTACCTGACGGCCTGTCGCCGTGGCAATTCGTGGCGTTTGGCTTTGTTGAATCAATAAAACCAGCCAAATTATGCGCCACTTGTCAGCCCAACTTACCGGCTTAGTGCCCTCGGCCGACTTTTCTTTCTTCTCCTTTTTGCCCTTGCCAGTTTCCGCCTCCTTGGCGGCGGTCTCTGGCACGACATCCTTGCTGCGCCGCTCTTCCGGTGAGTCGCCCATGATCACA

Search Again