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CRIMIC CR00907

Gene ade3

CG31628

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:7023308..7023308	27D4	FBti0202658	81154

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTAAAAGCTTAGCCGGGGAGTTAGC	TGTGGCCGATGAGTTTGGCTATCATC

Images

Construct Design Sequences

sgRNA sequence	ACGAGCACCTTTATGCTGAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CATACGATCCCCCAATCCCG	TTTATGCTGAGCGGCAGTCA	GCATAAAGGTGCTCGTCGAG	AGACAAACATAATCGCGCGC

 

Amplicon 5'

Amplicon 3'

CATACGATCCCCCAATCCCGACCGAGCACCACGCCCAAGACCATTTACCTCACCCATCCGCCCACGACCACGTCGCGACCTCTGCGCCAAAGACGAGCTCTTCCGACGCACTGATGATCGATGGACGTGATTCTTTGGCGGGGCAAGGGGCTGGTCTCTTCGGCGGCCAGCGGGCGAATCTGTGAATTTTGATCTAAACAATTAATTAAGCCACGAACAATAAATAGAAGTGCTAAGCAAACATAAGCTAAAGTGTAATCGATCTGTCGAGTTGTCTGCTGGGGATCATGGATCACATCATGGAGCGACATAAACAATTTTGGGTATTCGATTCTGTTTATGGCAGAGCAATCAATTTGAAAGAAGTCTGTCCCAAGCATTCCATTGAAATGCGAGCAATTGAGACTGAAAGTTAATAATTGATATTGAGTTTAAGAATTAGGGGAATCCTATTAAGCTGTTGAACCAATATATTTTGGCAATTCAAATTTCCATATTTAATAACGAACCAAATAACACCTGTTGTCCGCGCTTTAAAGTCTATAAATGATGGGATCGTAAAAGTCGCATTTGAAAGATATAATTTAAAGGAATAAAAAGTGGAAAGGCCCAATTTAAAGGTAATTTTTGTATAATCTAAAATCGAATCTAAAATTTTTGCCACTGCTACCTGCTATAATTAATTAACCGAATTAAAGCAGATAATGTATTAAAACTGGTTTTTGGTTCACTTTCAGTTGTAGTTTTGTGTATTTTTGTGTATACCATTATTTAAGTTTGAAAAACGTTGTTAAATGAGTACTCAATGAACAAATGTATATGTTGTAATTTAATTTACTAATATGTAGATTATATATATATACAACAAATTAATACTTTACAAAATTAAGCAGTGGATGCATAGTGCAAAACTTTTTAAAAATATATTTCCAATAACGAGATAGATCACACATCACTTATCAGCCAGACGTGCAATCGCGCCCCCATGGCCCGTCTAAGGGTTAAGGCGTCGCGACGCACCAAGATTGGATGCAATGAAACGTTTTCCCAGCGTCTCGATTACGGATTTATGACTGCCGCTCAGCATAAA

GCATAAAGGTGCTCGTCGAGCATGGAGTGCGTTTCTTACCACGACCAGAAGCCATAAAAGCGAATAATATCACTGGGTAACACCATTAAATGGCAACCAAACGCATTAAATGACGATAAATGAGCACCCGCTACCCAATTAACACACACATTTGCACATTTTCTTAGTAACTTTAGGAACCGCACGAAGGTCGCTCCAAGGTCGGCGTAACGAACCTACCTCGAAATCCTTAGGATCCAAGGTCTTGGCATCCAGATTCCGGCACTTCTCCACCAGCTGGATGCCATGGCTACCCGGCAGTGCGTATATTTGGGCCACCTTCGGAGATTGCGACAACTTCCAGCAGATCGCGTGCTCCCGACCGCCGCTGCCAATGACCAGAACGCGATGCGACATAATTCCGCTGGAGTGGAGGAACCACTAATCAGACTAATTCGACTTAATCACGTTTCCCCAGAGCAACAATAACACCAAACTAATGCGCACCCACGTGTTGATCGAAAAGCACGCGCAATTCGAAGTTTAAAGCCCGGTTCGGTTTCGAATTCGGATCAAACCGCTCGCTTCGACGCCTCTTTTTCAACTAAAATCTGGAATCGAGAAGTTGGAAACTAGGTAGGCACAGTATGTGTGTATGTATGTGCATAAATGTGTGAATATGAATACCCTATCCCATGCGCGCGATTATGTTTGTCT

 

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