CR00908

CRIMIC CR00908

Gene CG4630

CG4630

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:13216532..13216534	49F10	FBti0199682	79354

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGCTTTGCTGAGAATTTCACGACGG	GTCCTCAAATCTCGTGGGCTTCATCT

Images

Construct Design Sequences

sgRNA sequence	TAGCCAGTTACAAACTTGTTAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGTTTCGCCCATTTGCTTG	ACAAACTTGTTAGGCCCATCT	ACTGGCTAATGAGTCGTATGAGT	GAGACATCTCCGTCGAGCTG

Amplicon 5'	Amplicon 3'
TCGTTTCGCCCATTTGCTTGCGCAGCGATTCCAAAAGGCGCTACAAACTCCTTAATCGCTTCGGTCATGTCAGCGATCGCCAGTTGTTTGAGGGTCTCTTTGGAAATTGAATATACATTTAGGCGATTGTTATACCAGAGATACCCATTTGTTAATATCTGAATTAGAAAATCATGCCATCTTTTTGTATACAATACTTTATAACGAGTATTTTTTAAGGTCAGCTACATCGCTTTATATAAATATATGTGTATATTGCTGATCGGTGGCAAATATGTCCATCCAAACAAGAATGAATCACTTTCCCAATGCAATCGCCTCTTCCACCTGTGAAGATAGGAATGTTTAGCACGGGGATTCTAATACTCCGAGTTATAACTTGCCGTATCCGGTAGTTTTCGGTTGAAGGTTTCCGGCAGTAACAGCGAGAGGAGTCCAGCCACGAGCGATAGTGTCCCGAAAAGTAGCAGAGGTAGTGGGTCATAGTACGAAGCCTGTCAAAAATCAATCCGGATTATATGATTTGCCCTGAATTCTGGACTGAACTCACCAATAGGGGCACAAATGGAGCCAGCATTGCACCGAAGCGGGCAAATGTGGACATCACACCAACGCCGCCGCTCCTAATGACCTAGGATAAACGAAATGGAAACTAACCTGCTGATCTGAGTAAATGAGTTATGTACCGTGGGCATCATTTCCGCTGTGAACGTGTAGATGACTGCAAAGGAGGAGGTGATGCCCAGTTTTCCGACCAGAAAGAGCGTCACAACCAGCCAGTTGGCACCTAGAAAACCAGGTGTATATTATTGCCAGGCAAACCGGAGATATGTTAAAGTGTAAGGCTATCCTTCTTGGGAATCTAGGGAACTTACAACTCCACGAGTGCCTACTCTCGACTCCAGTCTGGCAGGGAATCCAATCTCCTTGCGCTTAGCGTGCATGCATGGTGATTTAGATCGTTATTATAATACTACCCTTTGATGAAAATGAAAAATGAATAATAATTTCACACCATCTACTATATGGAAATCAGTCTAGATGGGCCTAACAAGTTTGT	ACTGGCTAATGAGTCGTATGAGTAATATTTAGTGATCTCAAAACTGATCGGAATCTGATTCATATCTTACCTAGTGTAACGAATCCGCTGGCCACACACGTGATAGAGCAGAGCAGCAAGGAGCCAGAAAGTGCCACACGTCGTCCAAATCTCCGCAGGAACAGCTACTGGCGGAGGATTAGGGATGAGATTAGTCACGTTTCCGAACGAAGGCGGTGTCAACCTACCCATGCGAGGCTGTAGCCGGGTATCTCTACGAGGCAAACCAGGGCGAAGTTTAGGTACTTGTTGCCACTCAAACTGGTGGCGTTGAGCGAGAGTCCATAGTAGACGATGGCATTGACGGCCCAAATCAGCAGCAATATGGCGTATCTGCCCATCAAAATGTGAGAGCGCGCCACCTCCTTGACGGCCAACCAGATCTTGTCATCCTTCTTTACGTGCAGGCCACCCTCGACATCATCCTCTTGGCCCGTTTCTGCATCAAGCTCCTGTTGCTTGAAGCTGGCCATCAGCTCGACGGAGATGTCTC

Amplicon 5'

Amplicon 3'

TCGTTTCGCCCATTTGCTTGCGCAGCGATTCCAAAAGGCGCTACAAACTCCTTAATCGCTTCGGTCATGTCAGCGATCGCCAGTTGTTTGAGGGTCTCTTTGGAAATTGAATATACATTTAGGCGATTGTTATACCAGAGATACCCATTTGTTAATATCTGAATTAGAAAATCATGCCATCTTTTTGTATACAATACTTTATAACGAGTATTTTTTAAGGTCAGCTACATCGCTTTATATAAATATATGTGTATATTGCTGATCGGTGGCAAATATGTCCATCCAAACAAGAATGAATCACTTTCCCAATGCAATCGCCTCTTCCACCTGTGAAGATAGGAATGTTTAGCACGGGGATTCTAATACTCCGAGTTATAACTTGCCGTATCCGGTAGTTTTCGGTTGAAGGTTTCCGGCAGTAACAGCGAGAGGAGTCCAGCCACGAGCGATAGTGTCCCGAAAAGTAGCAGAGGTAGTGGGTCATAGTACGAAGCCTGTCAAAAATCAATCCGGATTATATGATTTGCCCTGAATTCTGGACTGAACTCACCAATAGGGGCACAAATGGAGCCAGCATTGCACCGAAGCGGGCAAATGTGGACATCACACCAACGCCGCCGCTCCTAATGACCTAGGATAAACGAAATGGAAACTAACCTGCTGATCTGAGTAAATGAGTTATGTACCGTGGGCATCATTTCCGCTGTGAACGTGTAGATGACTGCAAAGGAGGAGGTGATGCCCAGTTTTCCGACCAGAAAGAGCGTCACAACCAGCCAGTTGGCACCTAGAAAACCAGGTGTATATTATTGCCAGGCAAACCGGAGATATGTTAAAGTGTAAGGCTATCCTTCTTGGGAATCTAGGGAACTTACAACTCCACGAGTGCCTACTCTCGACTCCAGTCTGGCAGGGAATCCAATCTCCTTGCGCTTAGCGTGCATGCATGGTGATTTAGATCGTTATTATAATACTACCCTTTGATGAAAATGAAAAATGAATAATAATTTCACACCATCTACTATATGGAAATCAGTCTAGATGGGCCTAACAAGTTTGT

ACTGGCTAATGAGTCGTATGAGTAATATTTAGTGATCTCAAAACTGATCGGAATCTGATTCATATCTTACCTAGTGTAACGAATCCGCTGGCCACACACGTGATAGAGCAGAGCAGCAAGGAGCCAGAAAGTGCCACACGTCGTCCAAATCTCCGCAGGAACAGCTACTGGCGGAGGATTAGGGATGAGATTAGTCACGTTTCCGAACGAAGGCGGTGTCAACCTACCCATGCGAGGCTGTAGCCGGGTATCTCTACGAGGCAAACCAGGGCGAAGTTTAGGTACTTGTTGCCACTCAAACTGGTGGCGTTGAGCGAGAGTCCATAGTAGACGATGGCATTGACGGCCCAAATCAGCAGCAATATGGCGTATCTGCCCATCAAAATGTGAGAGCGCGCCACCTCCTTGACGGCCAACCAGATCTTGTCATCCTTCTTTACGTGCAGGCCACCCTCGACATCATCCTCTTGGCCCGTTTCTGCATCAAGCTCCTGTTGCTTGAAGCTGGCCATCAGCTCGACGGAGATGTCTC

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