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CRIMIC CR00912

Gene CG15141

CG15141

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:17412548..17412562	36C7	FBti0199684	79356

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTGGTTCCACTTGCCGCACATGAAA	AATGAGTGCCATAAGCATACCGCCAC

Images

Construct Design Sequences

sgRNA sequence	CATTAGGCAGTCGAGGCCCTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAACCAAAGGCACGACTTG	ATGTACTGCGGCATGAAGCT	CCCTCGGTGAAGAACTCCAA	GCCCAATCCCATCCAGAACA

 

Amplicon 5'

Amplicon 3'

CCAACCAAAGGCACGACTTGATTATTACCAAACCCTCGTTCCGTTGCATATTCAGGGCAAAAGCCTGACACAGTGGTATTACGCAGTCAATGGTGTCCAGAAAGTCAATAGCATAGTTATAGTCGCTCCTCTTGGCCAAATTGACTTTGAGATCATTGCGGGGCAGGCGATAGAGCTGGTAATATGATTGGTTGTCGATCTGCAAGTCATTCAGTCCACGAAACATCTGGTTGGGTATAACACGACCATCGGTCATCTTGTAAAAGGCTCCGCGTGGGCAAATGGCACACTCCTCTGTTATAATAAACACAATGGCTGCCAGCCGTTCCTCCTCCTTAAGCTTGACCACTTCAGGGCGGCTGATACCAGCCACCTGATTGCCTACGATGTCAAAAGTGGGGTCCTGTGTGCGTACAATATCAGAAAAACCACCACCACATTTACGTACTATCAACTCACCAGCTTAACGTAGGTCATCAGGCTGGGATCTCCTATAAATGGCTCCCTTGCCAATATAGTCGCCTGAAATATCTTGGGGCTCTGTACGAACGGCAGCAACTGCCATTGGAACTGGTCCAGACTGTAGAAATACTTGCGATCCTTGAGACAGTCATTAGTGTAGCCAAAGGCAATGTAGTAATCGTTCTTGGTGGCCGTAATGCGACCCCAAAGATACATGCCCGTAAAACGATTGTCATTTTGTAAAGCGATCAGCGAGTTTTCAATCAAGATGCGCTGCTCCGGCGACAGCTTCATGCCGCAGTACAT

CCCTCGGTGAAGAACTCCAAATTCATGACGAAAGTATCTTAGCAATTTATATGTGAAGTGTTGAAATAAAATATGGATTTAAGTGCTGAGAGTATTTGAAAGAAAACTCAGAACCGTAAAAAAAAATATGTTAGCGGAATGGATTTTTAATTACATGGCATGCATAAATCAGCACAAACTCTTTGAACTGCCAATTTCCCCCATTTATTCATCTCTACCCTTTCTCTTTCAAGGAGCTGCTTTCTGGACAAATGACTGGCGAAAATCACTCTGTCAGTGCTCCGAATGCCTGTCGCTTTACAAGGAACTTGCCGTGGAGTTTCTGCTGGATGTCGAGGATTCGGCTAAGACGTACGAGGAGCGCGGCATGAAGCGGGCTGAAGAGAATTCCAGTTATGAGCAAGGAATCCGGGCACTGGCCTCCATTGACCGGACACAGCAAATCGATGCGATTACCGAATACAATCGCATGAAGGACAAGCTAAAGGAATACCTGCAGGCATTCGCCGCCAGCAAGAAGGTAGTGACCGAGGAGGATATCAATCGGTTCTTTGCCGGCATGCGCAATGAGAACAATGCCAACCTGGGTCAGCCCTATTTCTGTCGCTAAACAGTTAGCACTCTATATTACTCTAGTATTACCCACATTCCCCCATGAAAGCGAAACATACATACATACATCATGTAAATTCTAAAGTTATATTTTATTTAAACAATAATCATTTTCAATTCACTTTTGTCAATAACGAAAAGTCGTAACAAGGCATCGGGCAATGAGATTCGCATTTCCCTCATTAAATTTATGTACATTGCGGTAAAATAGAATTATATAAGACAGGTTTTGTGTTGAAGTTCATTTTGGATTGAGACCTTTTTACATGTAGCTCTAATTGGTTAATTTCCGATATATTTTATGAAATATATGTACACTAGTATTTAGCAAAGTTTGTTGTTCATTTGAATGGCTGAATTCCCAAGCTATTGAGTTCTGCCTCAATGTCCTGGATAAGTTCGCTGGTGTCAATGGGCGCCATGCCTATGCTGCTGGAAATTAGATCACTAAACGATTGGGTGGGTTCATCTGCTGGAGGTTGCTGCTCCTGTGGCTGCTGTTGCTGTTGGTACTGCGACTGTTCTGGATGGGATTGGGC

 

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