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CRIMIC CR00915

Gene CG3860

CG3860

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:23994102..23994117	60B1	FBti0202659	81155

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	GTTTTGCAGTGTTGGGAAGCCCCAAA	AGTGTCAGCGGTGGTTTCATGTTGCT

Images

Construct Design Sequences

sgRNA sequence	TTATTGACCACTAAACGATAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTTGGAGGGTCTGGGCTTAC	ACGATAAGGGTGTTGCTCCTG	AGGAAACATAATCAGCGAGTACT	CGAGCCCTGGTCACAAGATT

 

Amplicon 5'

Amplicon 3'

GTTGGAGGGTCTGGGCTTACAGCTCCACAAGGTCGTCGAGTCGGGAATGTCAAGTGAGTACGCGGAGTCACTCTTCGGGATGTCGCCTTCCTGCTCCATAGGTGGATAGCTATCGCTCTGAAATGTAACAATTTTTGAGATACTCATCGCGGGTAACTCAAGAAAGTCTTACACTTACGTCTTGAATGGACAGGCTGCGAAATGAGTTTAGCTTAAAACTATTCAATTTCGAGAACATCTTCTTTGGAGTTCCGTTCGGAGAGCCATCGTAGTCATCGTTCTTCCTGGTTCCCTGCTTCAAAAACTGGACGTAGGACTCGGCAGAGCAGCATTTAATGAACTCGGTCCATTTCCCGTAGAGGAAGTATAGATTGGTTTCACTAGGGTTGATTTTTGTCTCGGATTAACAACTAGCCGAACTTGTTTAAAGCTATATGTATTTACCTAGCATCCTTCACAAATCCCTCTACTCGGTGCAAGTTTTTAGCTCCAGATCCGGCGGACTTGAAGGTGAGACTAGCCACATGGCCAGTGGCGTGGTTAGTGATCTCCATCTTTCCATACTGCTCAATCCACAGCCTGCCCACTATTATGTTGTGTACACAGCAATTGACATTAGTCCAACTGTAGCTCTCGTTCCATCTGGAAAAACACCGAGATAAGTGTTAATTTTTTTGCTAAAACGTATACAAGGAAACCTACTTTGGAAACTCGACGGTCACAGTTCCCTTCGGATTCACCTCAACACTCTTGCCCCAGAACTTGATTTTTGGGCTAATCGTGCCGTGGAACTTGAAGTCCTTGGACTCTGCGTGAAATGCAGACACTGGAGGATGGTGGGAAACCTGCTCGCAGACGATTCGGTAGTCGCCCTTCTGCAGCTCGTATGTTTCACCAAGCAGAGGATTAAAAGGTTTTCCCAGGCGCTCCCAGTTGGAGGCTAATGCTGATACGGCAAAAGCTGAAAGATAATTTTTAGTAAGAACATATCGCACACAAATGTATTACAAATTAACTGAAGCAGATAAAACATTCACTTTTGTTGGAGATGTATGCATATTTCGAGATGTAGTCATTGAACTCAGTATAAAGACTCATAAATTCAGTGCGGAAAAGGAATCCATTACCACAGACAATACGTTTTGCAGGAGCAACACCCTTATCGT

AGGAAACATAATCAGCGAGTACTCTATCCGGCATATAAACTATTATAGGTACAGACAGGAATCGGTGTACATTTGGAATAACGCCGATCACTTACAGGTGGGTTGATAAGCCCTAACTTTTGATAACACTCACCCGCCACGTATTTCATCCGATCTGCCGGGTGTTCCTGCTGAGCTGCTTCGGTGAGCAGTTGTGCGTACTCCATGTACTCACAGAGCCTCTGAAGGAAGCTCAATGGTTCGTTCAGCATGACCGGCATGGTGATTTTGCTCAGGTCCTTGCCGATGCAGTTCTTCAGAATGGCCCAGATGCTTACCTCCTTGCGGGAAATCATCGTGGCCGGAAGCTCAGTTCTGTGGGTGATAAGATATGGCGCGGATGAGTAATGTCAACCGGAATCACTTGGACTACGCACCTTTCCACATGCTCTGCGTAGTCGCCCTCTGCCATCTTGGGTGTGGAAGCAGGTGCAGCTTTAATCGACTGGAATCAGATTGTTATAGCGTGTCTCTGTGGGTTAATTGATTGCTAGTTGACCCTCTGCTGTGGAGACCAATAAGTTTACGCACGGAAAGACATGTGAGTGGAATTGCGATGATGCGGGCCAACGGTGGGCCAGTCCGAAATCTAGACAGGATTATACTCTGAAGGTGAGATAAGCCGATAATTTTGGCTGTGGGAGTGGGTACCCACACATGTGCAATTCATACCTTTTACTCGTTTTCTACTTTTTCGATTTCGATTGATTGTAATCTTGTGACCAGGGCTCG

 

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