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CRIMIC CR00917

Gene Dhx15

CG11107

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:7388560..7388562	43B2	FBti0206846	83200

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAAATTGCTGCCGTGCTGTTGGTTGT	AGTATTCACGGCAGCCACTTTAACGG

Images

Construct Design Sequences

sgRNA sequence	ACTTTCCGAGTGCCGAAGGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCATGTCCAAGCGTCGAATC	TTCGGCACTCGGAAAGTTTA	GGCGGCAAATGCTTCGTATT	AGCTGCGGATCCAATGGAAA

 

Amplicon 5'

Amplicon 3'

GCATGTCCAAGCGTCGAATCGAAGTGGGGGAGACCTACGGCAGCAAGGCGAAGAAGTAAGTGACCCGAGGCCAACATACGGCAGTAATGTAACGCTCCTTTCTCACCAGGGACCCGGAGGCGTCCTCCTCGTCGGCGGCAGCCGCTGCCGGCACAGTGGCTCAAATCCTGGGCGGATTCGTGCCCAACAAGCAACCGCCCACGATGAACCCGCTGACCAACACGCCGTACTCACAACGGTACCAGAATCTCTACAAGAAGCGCATTGCGCTGCCCGTGTTCGAGTACCAGGCGGACTTTATGCGGCTGCTCAGCCTGCACCAGTGTATTGTGCTGGTGGGCGAGACTGGCTCCGGCAAGACGACCCAGATCCCGCAGTGGTGCGTGGACTTCGCAGTATCCAAGGGGCGCAAGGGAGTCTCCTGCACACAGCCACGTCGAGTTGCCGCCATGTCCGTGGCCCAGCGCGTTTCGGAGGAGATGGACGTAAAACTGGGCGAAGAGGTCGGCTACTCCATCCGTTTCGAGGACTGCTCCACGGCCAAGACGCTGCTCAAGTACATGACCGATGGTATGTTGTTGCGCGAGGCCATGTCTGATCCCATGCTGGACCAGTACCAGGTCATTCTGCTTGACGAGGCTCACGAGCGGACCTTGGCCACTGACATTCTAATGGGCGTGCTCAAGGAGGTCATTCGTCAGCGCAGCGACCTTAAGCTGGTGGTCATGTCCGCCACACTGGACGCCGGCAAGTTCCAGCAGTATTTCGACAACGCACCGCTCATGAAAGTGCCCGGTCGCACACATCCCGTGGAGATCTTCTACACGCCCGAACCAGAAAGAGATTACCTAGAGGCTGCTATTCGCACGGTAATCCAGATACACATGTGCGAGGAAATTGAGGGTAAGTTGGTAAATGAGTTTAGCTTGGAGTTCATTTAATAGCTTGCTAGTTCATTGTTCTAAGAAGCATTTATCGATCATATGTTTACAATGTAAAAGTGCCAGGCAAAATATAAACTTTCCGAGTGCCGAA

GGCGGCAAATGCTTCGTATTTGCTTTAATGGTATTGTAATCATTCAAGTGTTTACGACTTTTGAATATGATCCTATTCAATGCCGGAAAAGACGCATTCGCTTTTTCCATTGTAAACTAAACCCCAACTTGATTTTGCTACTCTAGGTGATATCCTAATGTTCCTCACGGGCCAGGAGGAGATTGAGGAGGCTTGCAAGCGCATCAAGCGCGAGATCGACAATTTGGGCTCAGAAATTGGAGAGCTGAAATGCATTCCCCTGTACTCGACGCTGCCGCCCAATTTGCAGCAGCGCATCTTCGAACCGGCCCCGCCGCCAAATGCTAATGGCGCAATTGGACGCAAGGTCGTGGTGTCCACCAACATTGCCGAAACCTCGCTGACCATTGACGGAGTGGTGTTCGTGATTGATCCAGGCTTCGCCAAGCAAAAGGTGTACAATCCTCGCATTCGAGTGGAGAGTCTGCTCGTCTCACCCATTTCAAAAGCGTCTGCGCAGCAGAGATCCGGTCGTGCTGGACGTACGCGTCCTGGCAAGTGTTTCCGTCTGTACACGGAAAAGGCGTTTAAAAACGAGATGCAGGACAACACCTACCCCGAAATTCTGCGCTCCAATTTGGGTAATGCCTAGATCCCCTGGAAGTATTACTCGATTTCTAATATCTTGTTTCTTATTTTTGAGCAGGCACTGTGGTTCTGCAGCTCAAGAAGTTGGGCATCGATGATCTGGTGCATTTCGATTTTATGGACCCCCCTGCTCCAGAGACCTTGATGCGTGCCCTGGAGTTGCTCAACTATCTGGCCGCCCTGGATGATGACGGAAATCTTACAGACCTGGGTGCCGTTATGTCCGAGTTTCCATTGGATCCGCAGCT

 

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