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CRIMIC CR00919

Gene Liprin-gamma

CG11206

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:22344004..22344014	58D4	FBti0199685	79357

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGGAGAAAGCCGCTTCCGTGAACAA	ATAGATGGTTTTTGGGGGACTTGGGG

Images

Construct Design Sequences

sgRNA sequence	CTCTGCCCGTTCCACATCCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCGGTCACAAGTTCATCCA	GGAACGGGCAGAGTACCTTT	GAAACACCTAAGCCGCCACT	TCTTGGCCTCCAAACGAGAC

 

Amplicon 5'

Amplicon 3'

CCCGGTCACAAGTTCATCCATGTGAATGACTGATGATGAACCCAAATTGATTGCACACACACCGCCAGGATAATCGTTATTCACATACAAATTCCCCTGTGGATAGGCATTATGCCATCTTTTTCAGCTTTTTTGCTTATTGATTGTCAACACGGATTTATTTTACGGCCAAGGATTTAAATGAAAATTTAATTAGCACTTAATGCGCGGCATGAAATCATTATAAAAGGCAAAAAAAATGCATACAGGAATTGAGGTCGAATGCTCTGCACTGTTTACCAAATAAAAACTACCAAATTGAAGAGTTAACTTTTATTCAAGTCCCAAAACTAAAGTGGCTGAATCACCACCCCCGCTCCCCGTTAAATTTTCAGCGTTTAAGTTTCGAAAGCCCAACTCGACCGCAACCACTTTGGGATCCACCGACTTTTTCACCTTCTCCGGAATGACCCCGCCTATCTGCTTCGTTGACTTTTCAACTGCAGCACTAAAAGCGATTGGTCGGAGCTCCTGGACGGACAGACGGACAGATGGGGGGGCAGATGGGCAAATGGTGCTTGTATCTCCAACGAACGGACGAAGGCCATTACCATCCATTTGTCAGATTTTTGAAGTGTGCATCACTTTGCCATTCTGATATAATCTGATGCGTTGACCAAGCCAAAAGACCAAGAGCAAAAAAAGGTACTCTGCCCGTTCC

GAAACACCTAAGCCGCCACTGCATCCTCGTCCTGCGGACCTAGTTGTTTTGTCCTGCTGTGGCTGGCTTGTGGCTCTTACCCTCGGTTGCCACTGGTAGCTTGCAATCTGCCTGGCCACAGGACAACATTTAATTTCTTGCCCCTTTTGTGCCGCCAAATACTTTAGCAATTGCATTAAAACAAAACAAATGGGATTCAATATTTTACGCAGCCGGCAGATGGAATTAAATCAGCGTGCAGGTTGAAAGGATTCAATCAGGAACGCTGATTTAATTCCTTTTTGGCCGGCTCCCATTTGGCATTCCCACAGTGGCCAGCATTTGTTCATTCATGCCGCAAAGATCCTTGCCCCATGCCACAGGCCTCTACCGTTTCTCCAACCAACCCCCCCTTGCCCAAGGAAACTCCTTTCTTTGCCCGCAGCAATCTGCTGATTGCTCCCTCCGCTCACACCCGCGCGCTGCGCGTTTACACATTACAAATGCATTTGCATACGCTGAGCGAAAAGCCAAATCCAAACAGTAATTAAAATAAAATTGTATTAAATCAAATTAAGAAAAAATGCTCCTTTGCTGATCGTTTAGGATCATTACCAGCAATAACTATCCCCAAAGGAATTATCTTTTTTAAGGTAAGTATTTTTTCCCAGTGTACAAGCACCCTTGCAACAGAAGCCGCCGCTATCCCCCGCACACTTACCGGCATTGAGTGCACTGCCACTGGAGGGGGCGTTGTGGGCGGGGCCGGCTGCCTGTGCCAAGTGACCAGCGGCAATTAAATTCGAGTTATTCGCATGGATCATGGCTGTGCCGGAGTTGCAAGTGCTGCTGATGCCGTTGCTGTTGCTGTTGCAGTGGCTGCTGCCGATGCCATTCGGCAATTGCTGCTGCTGCTGTTGCTGCTGAGGCACTCCTCCTGCGGCTTCTGCTGGCTTGCCAGGATACGCAGGACTCCGGTGCTGGTGGTGCTGTAACTGCTGCTGGAGCGGCTGATTATCAGCGGTCATGGGTGGGGTGAGGGTCTGGTTGCTGCCATTCGAACTGGACGGCGATGAGCTGCTCTCGTTGCTGGGCGTGAGGTCTCGTTTGGAGGCCAAGA

 

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