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CRIMIC CR00923

Gene vlc

CG8390

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:5703007..5703023	41F8	FBti0199688	79360

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCGGCTCGGTTTGGCTTACATGAAA	TAGCACCAAGAGACGGCTTTGTTCCT

Images

Construct Design Sequences

sgRNA sequence	AACCCGATGTAATCCACAGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAAACTTCGAACGCCACCGA	TGCAAGGCGATTGAAGTTGG	ACATCGGGTTGATTTCCCCG	CGATGGGTTTGCACTTGGTG

 

Amplicon 5'

Amplicon 3'

TAAACTTCGAACGCCACCGACATCATCATGTTTTTCATCTCACCGAATTATAAATAGTTGTGTGTCGTTTGTTGGTGCCATAATGAATTCTACACGGGGCCATGGGAGGGAAAAAGTACGGGAACTGGGCGAAGCATCAAAGAAAAGCCAGCAAAGCGGAACTTTCAAAGTGCCGTTAAAGCTGTTCCCATCTGCTAAGCTTACACTTACACATTCACAGGCTTAACTTAGAAGGCGTAGTGCGCTGCTTATATTATTGAATTTGACTGGTGTTTCAAAATATATGGATCTGATTGGAGAGATAGAGGAGATCTGAATCATTATGTTTTGGGTTCCGAATAAGATAAATAAAAATTACCTGTCAGGCTCTTTCGATTCTCTGAATGCTCAATTTCCACATTAAAACCAAGTCAGACGTGGGCGTTATCAGTTTATTCGTGTTTATTCCTTTTGGAGGTCAACCACTAACGGCTAATTACGGTTAAGAATTGTAAATTGCATGAATAACTAAGCCCAATGCTTTTTATACAACAGGCTCAGTTTTCATGGATCGCAGTCTGGACTCTATTGGCAGCTGTTCACTGGATGTAGACGCCGACTCCTCCGATATATCAGGTAAATATTTATATGGATATTTATGTGGATATACATATAACCAACTTCAATCGCCTTGCA

ACATCGGGTTGATTTCCCCGAATTGCAGATACCAGTGGGAGCCTGAACTTTCCAACGCCGGTCTCAGTAAGGGACATAACACGCGAATTTACTGCCAACCTTCGGGAGCGCTGTGTGCCTAACTTGCCTCCCCAGCCTCAGCACAAAACCCCTGCCTACGTTGAATCCAGGTCGTCAGAGCTGGAGCACATGGTGCTGTCACCTGCAATTGTGGAGTGCGACCAAGAGCCGAGCTCCAATGATTTTGACGAGACTTCTGCAGAAAATTCACAAGAAGCTCCCGAAAAGAAGCCCAGCTATTTAAACCTGGCTTGCTGCGTCAACGGTTATTCCAACCTTACCACCTACGACTCCAAGATTCGGCAGGACATAAACAAGTCACGTGAGGTTTCCCCGATTCGTCCCTCCACCAGCAGTCTACAGTACTGCAAACGCAACCACTTTCTAGCGACACCAACTCTGCACAGCATGCCTTCTCCGGCCACCAAGTGCAAACCCATCG

 

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