• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00924

Gene CG2614

CG2614

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:20656622..20656622	38D2	FBti0199689	79361

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAATTTGCCAATGGTGACAAGTCCC	TTATGCTGTCGGCCAACTCTTGCTTC

Images

Construct Design Sequences

sgRNA sequence	AGAAGTCTGCTCGAGCTTAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAACACACACCATTGACCC	CCCCAAGAAGTCTGCTCGAG	CTCGAGCAGACTTCTTGGGG	ATAAACGCCGCGTATTTGCC

 

Amplicon 5'

Amplicon 3'

GCAACACACACCATTGACCCGGATGTGATTGACACGTCGCACTTTCTCAAACGCCCGCTGAAGGACGCGAGCGCGCGATGGACCCACGCCGCGGAGCAGCACCAGCAGGCAGCTGTGGTGCAGGGCGCTCTGCTCGTAGTCTGGGCGTGACAACGCTGGCTCCATGCCACCAGCACCGTGCGACAGCATCAGGCCAACGGCCGCACGCATTTACACCGCGCCGCGGCTCTCAAATCCAATCCCTGTCCCAGTCCGGAGGATGCGAGCGCCAGTTTCCTTTGCATCTGCCAAGTTCTTCACAGGCGTTCCATACCGCTGTCAAGTGGATAAGCTCTCGATTGGATTCGATTTCTTCAGAGGAGCCACCTCCTCCCCCTTTTCGAGGTCAGTCAGCGCCTGCTTTCACCACGCTTTTTGCCTGGTTTCTCGGCTGTTTCCGAATCCGCTGCACTCCTTCACATGGGTACCGATGCCGGGTACTCGGAGGAAGTCGAAAACCCCAGGAAATAAATGACTAAATGGCTTTTTAAGCGCAGATAATCGTTTAGCCGTTTGCTTTGGCAAAGAAATTGTCCAATCAGTGCCTGTCGACTAGTCGCTGTGAATGGCACATCGCACAGATTGACGGCAGAAAGGTCACACTAATCTAATCGATGGTGTTGACAATCAGATAAAATATTTGTACAAATATAAAATGGAAAACCAAGTTTCTTATAGTGCTACTTAAAAAAACTTTAATTGTATTACTTTTAGTATTACATTTAGCGAAATAAGCTATTGAATATTTTGCAAATGATCCGCCTCTAACTTATCGATACTTTTTGGCTCCGGCCACACTGCTGAGGGACCATCCACTAGAATGGCAATGCCCCGTACAATCACATTTTGCCGCTTTATTGTTTATATTTTGCATCCTTTTTGTAGTTGTTGAAACCATGAATTTACTGCCCAAAACCCGCGAGGAGTTCGCACAGACCGATTACTGGAACGAATTCTTCAAGAAGCGCGGTGAGAAGGCCTTCGAATGGTACGTAAATGGTACCCACTAAGCTCGAGCAGACTTCTTGGGG

CTCGAGCAGACTTCTTGGGGAAGGGAAGAACTCCGAGCAACATGTGTTTTCTTGGCCAACTAAAAGTTGCTTTAACTGCGAAGGTACGGCGAATACCTGGAGTTGTGCGACCAGATCCACAAATACATCAAGCCGGCGGATAGGATTTTGATGCTGGGATGCGGTAACTCCAAATTGAGCATGGATATGTACGACACAGGATTTCGGTAAGGAATCTTCTGTAATAAGCTTTATGTATAAGTAGCATGCGCCTCCTCCTTACATATAACATTCCTTTCCAATATGTATTAGATCTTGATCTTGGCCCTATAAATAACTTTCCCTTGGTATTTTACATTTTCCCCTTAGGGATATCACAAACATAGATATATCTCCCATCGCTGTGAAGAAAATGCTGGAACTGAATGCCAAATCGCGGCCGGAAATGAAGTTTCTGCAAATGGACGCGACAGCAATGACGTTCCCGGATGAAAGCTTTTCGGTGTCGTTGGACAAGGGAACGCTGGATGCTCTGTTTGCCGACGACGAACCTGAAACGAGAGCGGTGGTGGAAAACTATTTCAAGGAAATACTAAGAACCATGAGGTGGGTAATGTGCACAGTCCCAGATCGAAGGTATTCAATGTCTCTATTTATGCATCAGGAACGGTGGTCGCTATGTGGGTATCTCCCTGCTGCAAGAGCACATCCTCAATTTCCTACTGGACTTCCTGCCCAAGCACAATTGCATGCTGAGGATTGTTCACTGCTTGGGGGTGGAACAGGCCAACAAAGAGAAGAATGCCGATGATGCCTTGACGCTGCCTGTTTTCGTAGTCGTCGCTACTAAATTTAAGAGCCTTCCAATGCCAGTGAGTAGACCCCTAGGCAATTAACTTTTTGCATTCAGATTACAATTTGATTTACTTGTAGGTACTCGAATTTGGTTTCGGCAACGACAAGATGCAAAGATTCACCACGGTCTCCGAACTGAATAGCGCAGTTTCATCAGTCCAAAAGGCCGCTTTGGTTTGCAACGGTCTAGCCAGATCCAACATAGCTGGTCACAATGAGGTGATCATGGATCTGCACCGACCATCTGAGCAAACCCCTCGCTATACTATCCACATACTGGATAAGCCGCCAGCTCGCGGCCTGGGCAAATACGCGGCGTTTAT

 

---

Search Again